scholarly journals Growth and sporulation of Metarhizium flavoviride var. Flavoviride on culture media and lighting regimes

2001 ◽  
Vol 58 (3) ◽  
pp. 613-616 ◽  
Author(s):  
Sideney Becker Onofre ◽  
Cindia Mara Miniuk ◽  
Neiva Monteiro de Barros ◽  
João Lúcio Azevedo
Keyword(s):  
Biological Control ◽  
Mycelial Growth ◽  
Culture Media ◽  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Continuous Illumination ◽  
Agricultural Pests ◽  
Dark Cycle ◽  
Metarhizium Flavoviride ◽  
Conidia Production

Entomopathogenic fungi from the genus Metarhizium are largely used for the biological control of agricultural pests by conidia spreading on the field. Although conidia production is well studied in M. anisopliae, only few research studies were done in M. flavoviride. The present work was carried out alming to evaluate the Mycelial growth and sporulation of the entomopathogenic fungus Metarhizium flavoviride var. flavoviride growing at 27 ± 2°C on Potato-dextrose-agar (PDA), Czapek-agar (CZP) and a complete agar medium (CM) under three lighting regimes, (continuous illumination, light/dark cycle and an black light/dark cycle) were investigated. A completely randomized 3 × 3 (culture media × lighting regime) factorial design with four replicates was used. The best mycelial growth and sporulation occurred on the PDA and CM media under continuous illumination (P <= 0,05).

scholarly journals Morphological and cultural studies of Septoria vignicola

2014 ◽  
Vol 19 (1) ◽  
pp. 83-89
Author(s):  
R. D. Rawal ◽  
H. S. Sohi
Keyword(s):  
Mycelial Growth ◽  
Culture Media ◽  
Agar Medium ◽  
Carbon Sources ◽  
Potassium Nitrate ◽  
Potato Dextrose Agar ◽  
Optimum Growth ◽  
Carbon Supply ◽  
Wide Range ◽  
Agar Media

Pycnidial formation starts six days after inoculation. These arę globose, dark and range between 59.28-109.20 μm in diameier. The pycnidial wall is composed of 2- 3 layers of brown cells. The pycnidiospores ooze 8 days after inoculation. They are filiform, straight to curved, hyaline, septate with 2-4 septa, ends pointed or round and 21.84-68.64 x 1.25-2.15 μm. Mycelial growth was most profuse on Czapek Dox's agar medium, whereas maximum sporulation took place on Coon's, Sabauraud's and Potato dextrose agar media. The fungus could grow over a wide range of temperature from 12- 36°C (optimum: 24 ± l°C) and showed no growth at 40°C or above. Pycnidial formation occurred at 16- 28°C with a maximum at 24 ± l°C. Out of seven carbon sources tested, sorbose was the best for growth and sporulation of <i>Septoria vignicola</i> whereas maltose was a poor source for sporulation. The fungus responded very well to the supply of carbon in culture media as no growth of the fungus could be observed when the carbon supply was with held. Aspartic acid supported optimum growth among all the organic sources tried, whereas among inorganic sources potassium nitrate was the best, last growth observed on ammonium nitrate.

Growth and sporulation of Cercospora cruenta and Cercospora canescens in culture

1978 ◽  
Vol 56 (3) ◽  
pp. 229-233 ◽  
Author(s):  
E. J. A. Ekpo ◽  
O. F. Esuruoso
Keyword(s):  
Vigna Unguiculata ◽  
Mycelial Growth ◽  
Culture Media ◽  
Conidial Suspension ◽  
Optimum Temperature ◽  
Complete Darkness ◽  
Agar Concentration ◽  
Conidial Production ◽  
Conidia Production ◽  
Apparent Variation

Ten culture media were tested for their effect on growth and sporulation of Cercospora cruenta and C. canescens isolated from field-infected cowpea (Vigna unguiculata). There was very little mycelial growth and little or no conidia production on most media. Oxoid agar supplemented with V-8juice supported best growth and conidial production. Flooding V-8 juice agar with a conidial suspension resulted in multiplicity of tiny colonies with resultant increased sporulation. Conidia produced in culture were slightly longer than those produced in lesions on field-infected leaves and the degree of septation varied with the type of culture media. The width of conidia showed no apparent variation regardless of the culture media. More active mycelial growth but less conidial production was observed at agar concentration of 3% than at lower concentrations. Optimum temperature for mycelial growth and conidial production was 24–25 °C. Growth and conidial production were inhibited in complete darkness but stimulated by light.

scholarly journals Use of water restriction in the inoculation of carrot seeds

2019 ◽  
Vol 41 (4) ◽  
pp. 416-422
Author(s):  
Ricardo Borges Pereira ◽  
Tiago B. Torres ◽  
Patrícia Pereira da Silva ◽  
Gilvaine C. Lucas ◽  
Jadir Borges Pinheiro ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Osmotic Potential ◽  
Mycelial Growth ◽  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Control Treatment ◽  
Infection Rates ◽  
Water Restriction ◽  
Potato Dextrose Agar Medium ◽  
Osmotic Potentials

Abstract: This study aimed at evaluating the method of inoculation of carrot seeds in a potato-dextrose-agar medium (PDA) added of mannitol. Alternaria radicina was the fungus chosen to test such methodology. The osmosis-controlling agent was employed to obtain the osmotic potentials of 0.6 MPa, 0.8 MPa, 1.0 MPa and 1.2 MPa. In comparison with the control treatment, no significant mycelial growth inhibition was noticed at any of the osmotic potentials considered. The seeds were distributed over the fungal colonies for infection, where they remained for 48, 72, 96, 120 and 144 hours, at the osmotic potentials of 0.0 MPa (control), 0.6 MPa, 0.8 MPa, 1.0 MPa and 1.2 MPa, respectively. Tests of germination, sanitization and emergence were performed to evaluate the effects of colonization. Regarding the germination, the different osmotic potentials resulted in similar percentages of abnormal seedlings and dead seeds. As for the sanitization testing, higher infection rates were observed at the osmotic potentials of 1.0 MPa and 1.2 MPa. The lowest percentage of emergence was found at -1.2 MPa, therefore suggesting that this osmotic potential (produced with mannitol) might be used for infecting carrot seeds for research purposes.

scholarly journals Growth response of Aspergillus flavus IMS1103 isolated from poultry feed

2016 ◽  
Vol 2 (2) ◽  
pp. 221-228 ◽  
Author(s):  
Monzur Morshed Ahmed ◽  
Md Fakruddin ◽  
Md Nur Hossain ◽  
Khandaker Rayhan Mahbub ◽  
Abhijit Chowdhury
Keyword(s):  
Growth Rate ◽  
Aspergillus Flavus ◽  
Growth Response ◽  
Culture Media ◽  
Agar Medium ◽  
Lag Time ◽  
Potato Dextrose Agar ◽  
Poultry Feed ◽  
Effect Of Temperature ◽  
Temperature Range

Aspergillus flavus strains were isolated from locally available poultry feeds. Effect of temperature, pH and culture media on growth of Aspergillus flavus was studied. Temperature ranged from 4-42°C (4, 10, 20, 25, 30, 37 and 42°C) was examined. Except for 4°C and 10°C, the isolate was able to grow for the whole temperature range. The growth was maximum at 25°C and was influenced with increasing or decreasing of temperature from 42°C to 20°C.The lag time was strongly influenced by the temperature at lower temperature level than at higher temperature range. Effect of pH on growth of Aspergillus flavus was also examined; from comparison of 3 different pH levels, it is concluded that at most temperatures pH 6.5 showed a higher growth rate and as a consequence required a shorter time to achieve maximum colony diameter. No significant variations in the lag time were observed. A natural poultry feed meal agar medium (FMAM) was developed in the laboratory and growth of A. flavus was compared with other 2 synthetic dehydrated media namely; Czapek’sdox Agar (CDA) and potato dextrose Agar (PDA). Poultry feed meal agar medium showed better growth response than Czapek’sdox agar and potato dextrose agar at all conditions. At 25°C and pH 6.5 found optimum for growth of Aspergillus flavus in feed meal agar medium whereas, temperature 30°C and pH 6.5 found optimum for growth for Czapek’sdox agar media and temperature 30°C and pH 6 showed high growth rate on potato dextrose agar. Poultry feed meal media showed high affinity for growth of mycelium and early spore formation than other media examined.Asian J. Med. Biol. Res. June 2016, 2(2): 221-228

scholarly journals Cultural variability among nine isolates of Urocystis agropyri on wheat

1970 ◽  
Vol 33 (3) ◽  
pp. 347-351
Author(s):  
SS Karwasra ◽  
MS Beniwal ◽  
ML Chhabra
Keyword(s):  
Key Words ◽  
Mycelial Growth ◽  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Corn Meal ◽  
Potato Dextrose Agar Medium ◽  
Flag Smut ◽  
Fast Growing ◽  
Different Temperatures ◽  
Slow Growing

Four temperatures and five different media were tested for cultural variability of nine geographical isolates of Urocystis agropyri (Preuss.) Schroet causing flag smut of wheat. Urocystis agropyri is extremely slow growing pathogen and each isolate responded differently at different temperatures. All the isolates/ collections grew well at 20°C after 45, 60, and 70 days after incubation. Ambala isolate was fast growing. PDA medium was found to be most suitable for the growth of all the nine isolates/collections. The average mycelial growth was also maximum on Potato Dextrose Agar Medium followed by Corn Meal Dextrose Agar, Oat Meal Dextrose Agar, Grain Meal Dextrose Agar and minimum on Wheat Meal Dextrose Agar. Key Words: Variability, media, isolates, wheat. doi:10.3329/bjar.v33i3.1593 Bangladesh J. Agril. Res. 33(3) : 347-351, September 2008

scholarly journals Screening of Effective Media for the Growth of Pyricularia grisea under In-vitro Condition

2021 ◽  
Vol 9 (3) ◽  
pp. 131-135
Author(s):  
Ajay Singh Kaurav ◽  
Keyword(s):  
Pearl Millet ◽  
Mycelial Growth ◽  
Agar Medium ◽  
Warm Season ◽  
Potato Dextrose Agar ◽  
Annual Rainfall ◽  
Pyricularia Grisea ◽  
Cereal Crop ◽  
Effective Media

Pearl millet [Pennisetum glaucoma (L.) R. Br.], belongs to family Poaceae (section Paniceae), is the world’s hardiest warm-season cereal crop with the annual rainfall of 150 mm to 1000 mm.Maximum mycelial growth was recorded in potato dextrose carrot agar medium (86.33 mm) followed by Pearl millet Potato dextrose agar (83.67 mm), potato dextrose agar (78.33 mm), Pearl millet carrot dextrose agar (71.67 mm), Pearl millet potato agar (68.33 mm), Carrot dextrose agar (60.67 mm), Pearl millet grain potato dextrose agar (58.33 mm), Oat meal agar (51.67 mm), Pearl millet dextrose agar (51.67 mm), Calcium carbonate agar (42.67 mm), Malt agar (41.00 mm), Water agar (37.33 mm), Pearl millet grain dextrose agar (35.33 mm), Pearl millet agar (30.00) and Yeast extract agar (28.33 mm) while minimum growth was recorded in Pearl millet grain agar (27.33 mm).

scholarly journals Incidence and Biology of Corynespora cassiicola (Berk. & Curt.) Wei. disease of okra in Bangladesh

2014 ◽  
Vol 42 (2) ◽  
pp. 265-272 ◽  
Author(s):  
Fakhruddin Ali Ahmed ◽  
Nazmul Alam ◽  
Abul Khair
Keyword(s):  
Culture Media ◽  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Corynespora Cassiicola ◽  
Optimum Growth ◽  
Potato Dextrose Agar Medium ◽  
Significant Incidence ◽  
Effects Of Temperature ◽  
A Minor

Eight commercial okra cultivars were evaluated to determine the magnitude of incidence of Corynespora cassiicola (Berk. & Curt.) Wei. Maximum and significant incidence of C. cassiicola was 14.86 in the cultivar BARI 1. In rest of the cultivars, the occurrence of this fungus was very poor to nil. Corynespora cassiicola appeared to be a minor pathogen for okra. Effects of temperature, pH and culture media on growth and sporulation of the fungus were investigated. Potato dextrose agar medium was found to be the most suitable for optimum growth and sporulation of this fungus at pH 7 and 25ºC. The conidial length significantly varied with the changes of pH. The correlation between pH and conidial length was found negative and highly significant. DOI: http://dx.doi.org/10.3329/bjb.v42i2.18028 Bangladesh J. Bot. 42(2): 265-272, 2013 (December)

scholarly journals PHYSIOLOGICAL CHARACTERIZATION OF Rosellinia bunodes AND SYMPTOMATOLOGY OF Rosellinia ROOT ROT IN POPLAR SEEDLINGS

2018 ◽  
Vol 42 (1) ◽  
Author(s):  
Heloisa Thomazi Kleina ◽  
Álvaro Figueredo dos Santos ◽  
Henrique da Silva Silveira Duarte ◽  
Edilene Buturi Machado
Keyword(s):  
Root Rot ◽  
Mycelial Growth ◽  
Populus Deltoides ◽  
Culture Media ◽  
Potato Dextrose Agar ◽  
Physiological Characterization ◽  
Leaf Spots ◽  
Lack Of Information ◽  
Poplar Seedlings

ABSTRACT Poplar (Populus deltoides) is a forest species that has several phytosanitary problems, such as leaf spots, rusts and root rots. Since 2010, the death of poplar trees has been observed in commercial plantations in the state of Paraná, Brazil, caused by the fungus Rosellinia bunodes. Due to the lack of information about this pathosystem, the aim of this work was to carry out the physiological characterization of R. bunodes isolates collected from P. deltoides and the symptomatological characterization of Rosellinia root rot in the same host. Experiments were conducted at Embrapa Florestas, located in Colombo (PR), Brazil. The mycelial growth of nine R. bunodes isolates was evaluated in four culture media (potato-dextrose-agar, V8-agar, carrot-agar and water-agar) and seven different incubation temperatures (8, 12, 16, 20 24, 28 and 32 °C). The symptomatological characterization of Rosellinia root rot was performed on six-month-old poplar seedlings. The most favorable conditions for mycelial growth of R. bunodes were observed in potato dextrose agar (PDA) at 24 °C. The symptoms and signs of the disease internally in the stem showed up at 7 days after inoculation (DAI); however, chlorosis and wilting were observed only at 14 DAI. Sexual and asexual reproductive structures of R. bunodes were not observed on inoculated plants.

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