scholarly journals Study on the isolation and identification of Pasteurella species from farm animal and human

2008 ◽  
Vol 2 (1) ◽  
pp. 81-90
Author(s):  
Sawsan salman Atia mubarak ◽  
Ismail khatham Shubar ◽  
Amar salim Rahiem
Keyword(s):  
Pasteurella Multocida ◽  
Agar Medium ◽  
Brain Heart Infusion ◽  
Farm Animal ◽  
Brain Heart Infusion Broth ◽  
Laboratory Animals ◽  
Isolation And Identification ◽  
Selective Agar

This study was interested in isolation and identification of Pasteuralla species in animals and human , also studing it's sensitivity to antibiotic with comparison to it's pathogenicity for laboratory animals . Tow groups of samples were collected and investigeted, 1st group consisting of one handered thirty six samples which were collected from animals ( cow .sheep , goats , and chikens ) . 2nd group was also consisting of 136 samples that were colleeted from human ( wound , urine , and sputum ) . These samples were cultured in selective enrichement brain heart infusion broth and then in the new Pasteurella multocida selective agar medium .

scholarly journals STUDY ON PATHOGENICITY OF PASTEURELLA MULTOCIDA WHICH ISOLATED FROM FARM ANIMALS AND MAN

2021 ◽  
Vol 26 (1) ◽  
pp. 74-81
Author(s):  
S. S. A. Mobarak ◽  
A. K. Shubber ◽  
A. S. Raheem
Keyword(s):  
Pasteurella Multocida ◽  
Culture Media ◽  
Agar Medium ◽  
Farm Animals ◽  
Pathogenicity Test ◽  
Infected Farm ◽  
Human Samples ◽  
Selective Agar ◽  
Suppurative Pneumonia ◽  
Animal Isolates

This study was described for the nature of the pathpgenesis of bacteria Pasteurella multocida which was isolated from infected man made comparison between these bacteria and those from infected farm animals. The percentage of Pasteurella multcida diagnosed bacteria from animals and human was 29.4% and 16.9% respectively. Comparing to other culture media Pasteurella multocida selective agar medium was characterized by its selectivity and sensitivity and then was attempt for biotyping species and subspecies of isolated Pasteurella from animals and human samples were successfully achieved. Pathogenicity test was performed on mice, only nine human isolatetes and twenty-one animal isolates from Pasteurella multocida were virulent. Todistinguish between the pathogenesis of human and animal isolates, one isolated from human and animal were chosed, in addition to the standared strain. The mice had been experimentally infected by three different ways, I/P, I/T, I/Eye. The results were showed that Pasteurella multocida can produce lesions as fibrinous suppurative pneumonia in lungs, liver and spleen which were detected histopatho logically. However the animal isolates were more virulent than human or standared strain.

A Simple Medium for Isolation of Coagulase-Positive Staphylococci in a Single Step

1982 ◽  
Vol 45 (3) ◽  
pp. 218-222 ◽  
Author(s):  
LEONIE MINTZER-MORGENSTERN ◽  
ELIY AHU KATZENELSON
Keyword(s):  
Agar Medium ◽  
Brain Heart Infusion ◽  
Egg Yolk ◽  
Food Samples ◽  
Selective Medium ◽  
Single Step ◽  
Isolation And Identification ◽  
Complete Reversal ◽  
Simple Medium ◽  
Coagulase Positive Staphylococci

An agar medium containing NaCl, egg yolk and tellurite for selective quantitative isolation of coagulase-positive staphylococci from food was developed. Isolation and identification of the staphylococci was achieved in a single step. A properly diluted food sample was spread over the medium and incubated for 24 h at 42 C. Coagulase-positive staphylococci appeared as small grey to dark-grey colonies surrounded by a dense white opacity. Coagulase-negative bacteria which, at times, grow on this medium, did not produce this reaction. The identification on this selective medium of isolates from 683 different food samples as coagulase-positive staphylococci was subsequently confirmed by the coagulase test. Comparative titrations of 29 various coagulase-positive staphylococcus strains on both the selective medium and nutrient agar yielded nearly identical titers. The growth of heat-stressed staphylococci was inhibited by the selective medium. Complete reversal of the inhibition was achieved by a 3-h pre-incubation in brain heart infusion at 37 C.

A Rapid Microbiological Method for Enumeration of Pseudomonas fluorescens from Broiler Chicken Carcasses

1997 ◽  
Vol 60 (4) ◽  
pp. 385-390 ◽  
Author(s):  
SCOTT M. RUSSELL
Keyword(s):  
Pseudomonas Fluorescens ◽  
Broiler Chicken ◽  
Brain Heart Infusion ◽  
Brain Heart Infusion Broth ◽  
Animal Origin ◽  
Plate Count ◽  
Isolation And Identification ◽  
Plate Count Agar ◽  
Spoilage Bacteria ◽  
Time Required

A method to selectively enumerate Pseudomonas fluorescens from fresh chicken carcasses in less than 24 h using capacitance microbiology was developed. Capacitance assays were conducted on whole-carcass rinses at 25°C using brain heart infusion broth (BHI) containing 25 μg of Irgasan per ml to obtain a detection time. The capacitance samples were spread plated on plate count agar for isolation and identification. From plates with the highest dilution, from each carcass, 4 colonies were randomly selected and identified. Seven species of bacteria including Pseudomonas fluorescens were responsible for capacitance detection times. Various antibiotics and chemicals were added to basal media or brain heart infusion broth with Irgasan and were evaluated to select for the growth of P. fluorescens. BHI broth containing 4 μg of nitrofurantoin, 120 μg of carbenicillin, and 25 μg of Irgasan, all per ml, was found to be optimal and was termed Pseudomonas fluorescens selective additive (PSA) (patent pending). In a second study, 12 carcasses were collected in each of three replicate trials. For each trial, 2 carcasses were sampled immediately and 2 were sampled after storage at 3°C on days 3, 6, 9, 12, and 15. The BHI-PSA broth was found to be excellent for enumeration of P. fluorescens from broiler chicken carcass rinses in assays using capacitance microbiology at 25°C. The time required to enumerate P. fluorescens for all samples (day 0 to 15) was <22.4 h. This method is rapid and would be a useful tool for determining the number of spoilage bacteria on fresh chicken and thus may possibly be used to predict the potential shelf life of fresh chicken and other foods of animal origin.

scholarly journals Imprint and spreading techniques for the isolation and identification of subungueal fungi in claws of healthy cats

2021 ◽  
Vol 15 (1) ◽  
pp. 30-35
Author(s):  
Camilla Freitas Oliveira ◽  
Jamille Bispo de Carvalho ◽  
Luiza Montenegro Cintra Castro ◽  
Paula Elisa Brandão Guedes ◽  
Katharine Costa dos Santos ◽  
...  
Keyword(s):  
Pathogenic Fungus ◽  
Brain Heart Infusion ◽  
Brain Heart Infusion Broth ◽  
Isolation And Identification ◽  
The Third ◽  
Trichoderma Sp ◽  
Fusarium Sp ◽  
Petri Dishes ◽  
Aspergillus Sp ◽  
Rhizopus Sp

The aim of this study was to compare imprint and spreading techniques for the isolation and identification of colonies of pathogenic and non-pathogenic fungus in the claws of semidomiciliated cats. For that propose, 150 cats were evaluated, subdivided into three groups of 50 animals. In the first and second groups, the cats were submitted to the imprint technique in Petri dishes containing Selective Mycobiotic Agar: In the first group, the cats were subjected underwent antisepsis with 70% ethanol of the claws of the thoracic limbs and in the second group the animals were subjected underwent antisepsis with 70% ethanol of the claws of only one of the thoracic limbs. The third group was submitted to the spreading technique, whose material was collected by rubbing a sterile swab moistened with brain-heart infusion broth, in the claws of the forelimbs, where an aliquot of the material was transferred to Petri dishes containing Selective Mycobiotic Agar. The material was stored at 25°C for 30 days. The readings were performed on days 5, 7, 15, and 30 post incubation. Using the imprinttechnique performed under the conditions of this experiment, we were not able to isolate and identify the colonies because since day 5, they were overlapped. From the spreading technique, Mucor sp. (54,34%), Rhodotorula sp. (28,26%), Fusarium sp. (21,73%), Aspergillus sp. (21,73%), Trichoderma sp. (19,56%), Penicillium sp. (19,56%), Cladosporium sp. (10,86%), Rhizopus sp. (8,68%), Acremonium sp. (6,5%), Exophialia sp. (6,5%), Paecilomyces sp. (4,34%), Trichosporon sp. (4,34%), and Geotrichum sp. (2,17%) were isolated. It was concluded that the spreading technique proved to be useful in isolating fungal colonies from feline claws, and the animals do not present symptoms, which signals the importance of them as possible sources of exposure for tutors. The cats were negative for Sporothrix sp. by the imprint and spreading techniques.

scholarly journals Development of an Improved Selective Agar Medium for Isolation of Yersinia pestis

2003 ◽  
Vol 69 (10) ◽  
pp. 5787-5792 ◽  
Author(s):  
Raphael Ber ◽  
Emanuelle Mamroud ◽  
Moshe Aftalion ◽  
Avital Tidhar ◽  
David Gur ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Quantitative Evaluation ◽  
Fluorescent Protein ◽  
Agar Medium ◽  
Brain Heart Infusion ◽  
Selective Medium ◽  
Bacterial Populations ◽  
Medium Components ◽  
Selective Agar ◽  
Green Fluorescent

ABSTRACT Existing media designed for selective isolation of clinically important members of the genus Yersinia were found to be unsatisfactory for the growth and isolation of Yersinia pestis. We report the development of a new selective agar medium (termed BIN) that supports the growth of Y. pestis. The development of the formulation of this medium was based on a fluorescence screening system designed for monitoring bacterial growth on semisolid media, using a green fluorescent protein-expressing strain. High-throughput combinatorial experiments can be conducted for the quantitative evaluation of the effect of different medium components on growth. Generation of fluorescence plots in this system, using microplates, allowed the quantitative evaluation of the growth rate of Y. pestis EV76 cultures in different agar compositions. The final BIN formulation is based on brain heart infusion agar, to which the selective agents irgasan, cholate salts, crystal violet, and nystatin were introduced. It was found that BIN agar is more efficient in supporting colony formation and recovery of Y. pestis than are the conventional semisolid media MacConkey agar and Yersinia-selective agar (cefsulodin-irgasan-novobiocin agar). The advantage of BIN over other media has been also demonstrated in recovering virulent Y. pestis from the mixed bacterial populations found in decaying carcasses of infected mice. The BIN medium is suggested as a selective medium for isolation and recovery of Y. pestis from various backgrounds.

Molecular Detection of Bacterial Pathogens Directly from the Nasal Swabs and Lung Tissue of Sheep and Goats

2019 ◽  
Vol 15 (02) ◽  
pp. 22-25
Author(s):  
Sunaina Thakur ◽  
Subhash Verma ◽  
Prasenjit Dhar ◽  
Mandeep Sharma
Keyword(s):  
Pasteurella Multocida ◽  
Direct Detection ◽  
Bacterial Species ◽  
Economic Losses ◽  
Isolation And Identification ◽  
Sheep And Goats ◽  
Histophilus Somni ◽  
Nasal Swabs ◽  
Mycoplasma Spp

Respiratory infections of sheep and goats cause heavy morbidity and mortality, leading to huge economic losses. Conventional methods of diagnosis that include isolation and identification of incriminating microbes are time-consuming and fraught with logistic challenges. Direct detection of incriminating microbes using molecular tools is gaining popularity in clinical, microbiological settings. In this study, a total of 50 samples (44 nasal swabs and 6 lung tissues) from sheep and goats were screened for the detection of different bacterial species by in vitro amplification of genus or species-specific genes. Histophilus somni was detected in 2% goat samples, Trueperella pyogenes in 20% goat nasal swabs, whereas 22% goat nasal swab samples were found positive for Mycoplasma spp. None of the samples from sheep was detected positive for H. somni, T. pyogenes, Mycoplasma spp. Similarly, all samples, irrespective, whether from sheep or goats, showed negative results for Pasteurella multocida, Mannheimia haemolytica, and Corynebacterium pseudotuberculosis.

scholarly journals Xylose-Lysine-Tergitol 4: An Improved Selective Agar Medium for the Isolation of Salmonella

1991 ◽  
Vol 70 (12) ◽  
pp. 2429-2432 ◽  
Author(s):  
RUSSELL G. MILLER ◽  
C.R. TATE ◽  
E.T. MALLINSON ◽  
J.A. SCHERRER
Keyword(s):  
Agar Medium ◽  
Selective Agar

THE EFFECT OF "COMPETASE" ON DNA UPTAKE IN PROVOKED TRANSFORMATION OF A STREPTOCOCCUS

1965 ◽  
Vol 11 (5) ◽  
pp. 823-827 ◽  
Author(s):  
R. Pakula ◽  
A. H. W. Hauschild
Keyword(s):  
Deoxyribonucleic Acid ◽  
Brain Heart Infusion ◽  
Streptomycin Resistance ◽  
Brain Heart Infusion Broth ◽  
Dna Uptake ◽  
Retarding Effect ◽  
Competence Factor ◽  
Effect On Growth

The competence-provoking factor produced by the highly transformable group H streptococcus, strain Challis, was used to provoke efficient transformability in the poorly transformable group H streptococcus, strain Wicky. Transformations to streptomycin resistance were carried out with C14-labelled DNA which was extracted from bacteria fed with thymidine-2-C14.When cultures of strain Wicky were grown in Difco brain–heart infusion broth, supplemented with serum, and treated with competence factor and deoxyribonucleic acid, 25 to 40% of viable units were transformed while no transformation occurred without the factor. At the same time, the incorporation of C14 into cells treated with competence factor was higher than incorporation of C14 into untreated cells.Crude preparations of the competence factor had a retarding effect on growth of the streptococcus, irrespective of whether DNA was added.

Assessment of different selective agar media for enumeration and isolation ofListeriafrom dairy products

1988 ◽  
Vol 55 (4) ◽  
pp. 579-583 ◽  
Author(s):  
Lucas Dominguez ◽  
José Francisco Fernández ◽  
Victor Briones ◽  
José Luis Blanco ◽  
Guillermo Suárez
Keyword(s):  
Dairy Products ◽  
Agar Medium ◽  
Raw Milk ◽  
Superior Performance ◽  
Direct Plating ◽  
Selective Agar ◽  
Natural Microflora ◽  
Agar Media ◽  
The Difference ◽  
Better Than

SummaryDifferent selective agar media were compared for the recovery and isolation of five species ofListeriafrom raw milk and cheese. The selective media examined were Beerens medium, MacBride medium and that described by Dominguezet al.(1984) with 6 mg/1 acriflavine, listeria selective agar medium (LSAM), and LSAM with 12 mg/1 acriflavine (LSAM × 2A); a non-selective yeast glucose Lemco agar was included for comparison. When the difference between listeria and the natural microflora of raw milk and cheese was 102cfu/ml, listeria could be isolated by direct plating on all media tested. When it was lower than 103–104cfu/ml, listeria were isolated by direct plating only on LSAM and LSAM × 2A. When the difference was greater than 104cfu/ml, a previous enrichment was necessary to isolate them. LSAM and LSAM × 2A media performed better than the other media tested for isolating listeria by direct plating and improved their isolation from dairy products. This superior performance was evaluated by the ability of these media to support colony formation of different species ofListeriatested, the easy recognition of these colonies from those formed by other microorganisms and by their capacity to inhibit the natural microflora of these foods.

THE PREDOMINANT BACTERIA IN NATURAL ZOOGLOEAL COLONIES: I. ISOLATION AND IDENTIFICATION

1967 ◽  
Vol 13 (12) ◽  
pp. 1671-1682 ◽  
Author(s):  
Richard F. Unz ◽  
Norman C. Dondero
Keyword(s):  
Waste Water ◽  
Single Cell ◽  
Agar Medium ◽  
Isolation And Identification ◽  
Group I ◽  
Yeast Autolysate ◽  
Conventional Methods ◽  
Group Ii ◽  
Produce Acid

Direct, single-cell isolations of bacteria, primarily from natural, branching, waste water zoogloeas, were made by micromanipulation. Isolations were also made by conventional methods. Direct isolates were classified, chiefly with regard to zoogloea formation, into two groups designated group I (zoogloea-forming) and group II (nonzoogloea-forming). Casitone – glycerol – yeast autolysate agar medium was best for the isolation of group I bacteria. Group I isolates reduced nitrate to gas, possessed urease and catalase, and gave positive oxidase reactions. They were generally able to hydrolyze gelatin but, with one exception, did not produce acid from carbohydrates, and none produced H2S, indole, or acetylmethylcarbinol or utilized Koser citrate. Group II strains were usually more diverse on differential tests and could be distinguished from group I strains. Group I strains were characterized as Zoogloea strains and were found to be the predominant bacteria in natural, branching, zoogloeal colonies.

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