Determination the causative strain for hydatid cyst in Iraqi cattle by using ND1 gene

Hydatid Cysts were obtained from 15 cows from liver, lung, spleen, heart, and peritoneal cavity, between December 2014 and October 2015. Hydatid cysts (protoscoleces) were used for deoxyribonucleic acid extraction by using mechanical grinder. The purification of mtDNA was done by (promega kit, USA). The mitochondrial NADH dehydrogenase subunit 1 (ND1) genes was used as targets for polymerase chain reaction amplification, all hydatid cysts yielded amplification products. Polymerase chain reaction product for NADH1 800 basic pair. The polymerase chain reaction products were purified and partial sequences were generated. The sequences obtained were found to align with corresponding region for ND1 gene in the Gene Bank nucleotide database confirming to genotype of sheep strain (G1) in Iraq, Phylogenetic analysis of partial sequence data from ND1 genes for obtained Phylogenetic tree. G1 genotype was the most common taxon and the actual source of infection of Iraqi's cattle. All of 15 strains were G1 genotype (sheep strain) based on the partial sequences of NADH dehydrogenase 1 (ND1).

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A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection

Micromachines ◽

10.3390/mi11020186 ◽

2020 ◽

Vol 11 (2) ◽

pp. 186 ◽

Cited By ~ 2

Author(s):

Xinchao Zhu ◽

Jun Zhao ◽

Anzhong Hu ◽

Jingyu Pan ◽

Guoqing Deng ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Silicon Dioxide ◽

Microfluidic Chip ◽

Polymerase Chain Reaction Amplification ◽

Signal Acquisition ◽

Acid Extraction ◽

Chain Reaction ◽

In Situ Pcr ◽

Polymerase Chain

The outbreak of Zika virus (ZIKV) has posed a great challenge to public health in recent years. To address the urgent need of ZIKV RNA assays, we integrate the microfluidic chip embedded with chitosan-modified silicon dioxide capillaries, smartphone-based detection unit to be a C3-system for the rapid extraction and detection of ZIKV RNA. The C3-system is characterized by: (1) four chitosan-modified silicon dioxide capillaries integrated in the microfluidic chip for target ZIKV RNA enrichment and “in situ PCR” (polymerase chain reaction) amplification; (2) smartphone-based point of care (POC) device consisting of a pneumatic subsystem for controlling the nucleic acid extraction processes in the microfluidic chip, a heating subsystem for sample lysis and PCR amplification, and an optical subsystem for signal acquisition. The entire detection processes including sample lysis, ZIKV RNA enrichment, and reverse-transcription polymerase chain reaction (RT-PCR) is achieved in the microfluidic chip. Moreover, PCR buffers can be directly loaded into the chitosan-modified silicon dioxide capillaries for “in situ PCR”, in which the captured ZIKV RNA is directly used for downstream PCR without any loss. ZIKV RNA extracted by the C3-system can be successfully recovered at very low concentrations of 50 transducing units (TU)/mL from crude human saliva. This means that our method of detecting viremia in patients infected with ZIKV is reliable.

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"Portraying" of plant genomes using polymerase chain reaction amplification of ribosomal 5S genes

Genome ◽

10.1139/g91-158 ◽

1991 ◽

Vol 34 (6) ◽

pp. 1028-1031 ◽

Cited By ~ 22

Author(s):

Alexander Kolchinsky ◽

Maria Kolesnikova ◽

Evgenii Ananiev

Keyword(s):

Polymerase Chain Reaction ◽

Higher Plants ◽

Polymerase Chain Reaction Amplification ◽

Reaction Products ◽

Coding Region ◽

Chain Reaction ◽

Chromosome Addition ◽

Chromosome Addition Lines ◽

Plant Genes ◽

Polymerase Chain

Nontranscribed spacers of plant genes coding for ribosomal 5S RNA were amplified using the polymerase chain reaction. Primers were synthesized that were complementary to 3′ (direct) and 5′ (reverse) ends of the coding region and that are universal for higher plants. The patterns of polymerase chain reaction products are species and, sometimes, variety specific. The use of this approach for identification of barley 5S genes in chromosome-addition lines of wheat is discussed. This principle can be applied for the "portraying" of other tandem repetitive genes containing divergent regions.Key words: polymerase chain reaction, 5S genes, plants, DNA polymorphism.

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Polymerase Chain Reaction Amplification of Archival Material for Parvovirus B19 in Children with Transient Erythroblastopenia of Childhood

Fetal and Pediatric Pathology ◽

10.3109/15513819609168684 ◽

1996 ◽

Vol 16 (3) ◽

pp. 471-478 ◽

Cited By ~ 8

Author(s):

Beverly Barton Rogers ◽

Zora Rogers ◽

Charles Timmons

Keyword(s):

Polymerase Chain Reaction ◽

Polymerase Chain Reaction Amplification ◽

Parvovirus B19 ◽

Chain Reaction ◽

Archival Material ◽

Reaction Amplification ◽

Polymerase Chain

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Faculty Opinions recommendation of Synthesis and polymerase chain reaction amplification of DNA strands containing an unnatural triazole linkage.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1158100.618201 ◽

2009 ◽

Author(s):

Scott Silverman

Keyword(s):

Polymerase Chain Reaction ◽

Polymerase Chain Reaction Amplification ◽

Chain Reaction ◽

Dna Strands ◽

Reaction Amplification ◽

Polymerase Chain

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Polymerase chain reaction amplification and typing of rotavirus in environmental samples

Water Science & Technology ◽

10.2166/wst.1995.0643 ◽

1995 ◽

Vol 31 (5-6) ◽

pp. 371-374 ◽

Cited By ~ 5

Author(s):

R. Gajardo ◽

R. M. Pintó ◽

A. Bosch

Keyword(s):

Polymerase Chain Reaction ◽

Environmental Samples ◽

Polymerase Chain Reaction Amplification ◽

Rt Pcr ◽

Chain Reaction ◽

Pcr Assay ◽

Group A ◽

Reaction Amplification ◽

Stool Specimens ◽

Polymerase Chain

A reverse transcription polymerase chain reaction (RT-PCR) assay is described that has been developed for the detection and serotyping of group A rotavirus in stool specimens and concentrated and non-concentrated sewage specimens.

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