CYTOLOGICAL CHARACTERISTICS OF APPLE COLLECTION SAMPLES – SOURCES OF VALUABLE SIGNS FOR BREEDING

Aim of this study was to examine the characteristics cytology of mucous cell-,temperature- and pH vagina during estrous cycle in local sheep. 31local sheep were synchronized with vaginal sponge consist of 20 mg progesterone hormone before carried out observations of cytology of cells from the vaginal mucose through vaginal swabs, temperature and pH of the vagina. Vaginal swabs were collected daily at 7 am for a weeks.Vaginal temperature and pH measurement is carried out twice a day, at 07.00 am and 15.00 pm for a weeks after vaginal swabs. Smears of the swab were then prepared on glass slide and they were stained with Giemsa. Vaginal epithelial cells; Parabasal, intermediate and superficial cells were counted and their percentages during pro-estrous, estrous and di-estrous were determined. Di-estrous was characterized by the absent of superficial cells in the epithelial vagina. Pro-estrous was characterized by the increasing progressively of intermediate/superficial cells in epithelial vagina, whereas estrous was characterized by the presence of superficial/cornification cells in most epithelial vagina. Based on the dominance of superficial cell, the number of sheep identified as estrous is highest on third day, with 52%. Observation on vaginal temperature also resulting that the highest temperature values obtained on the third day of 39,08±0.28°C. It could be effected of the vaginal pH during the observation. Underthe influence ofestrogen, the epithelial vaginalcellssynthesizeand accumulateglycogenin large quantitiesdepositedin the lumen ofvagina. Vaginal bacteriametabolizethe glycogenformlactic acid, which causesvaginal pHis low.The pH conditions prevent from pathogenic microorganisms and fungi. Increased estrogenal so cause cell proliferation through the thickening of the epithelium lining of the vagina so that the cells differentiate.Increasing of glycogenin the superficial cells, and ceratin cells found in the cytoplasm of cells, as well as acidic pH in the lumen vagina causing changes in the chemical composition of each cell (Zaid, 2011).It can be concluded that during estrous phase, cytology of vaginal mucose cell is dominated by superficial cell and vaginal temperature is increasing as well as pH of vagina.

Download Full-text

Miscanthus: Inter- and Intraspecific Genome Size Variation Among M. × Giganteus, M. Sinensis, M. Sacchariflorus Accessions

Acta Biologica Cracoviensia s Botanica ◽

10.1515/abcsb-2015-0013 ◽

2015 ◽

Vol 57 (1) ◽

pp. 104-113

Author(s):

Sandra Cichorz ◽

Maria Gośka ◽

Monika Rewers

Keyword(s):

Genome Size ◽

Dna Content ◽

Nuclear Dna ◽

Size Variation ◽

Nuclear Dna Content ◽

Interspecific Variation ◽

Genome Size Variation ◽

2C Dna Content ◽

Cytological Characteristics ◽

Stomatal Length

AbstractSinceM. sinensisAnderss.,M. sacchariflorus(Maxim.) Hack. andM. ×giganteusJ.M.Greef & Deuter ex Hodk. and Renvoize have considerably the highest potential for biomass production amongMiscanthusAnderss. species, there is an urgent need to broaden the knowledge about cytological characteristics required for their improvement. In this study our objectives were to assess the genome size variation among eighteenMiscanthusaccessions, as well as estimation of the monoploid genome size (2C and Cx) of theM. sinensiscultivars, which have not been analyzed yet. The characterization of threeMiscanthusspecies was performed with the use of flow cytometry and analysis of the stomatal length. The triploid (2n = 3x = 57)M. sinensis‘Goliath’ andM. ×giganteusclones possessed the highest 2C DNA content (8.34 pg and 7.43 pg, respectively). The intermediate 2C-values were found in the nuclei of the diploid (2n = 2x = 38)M. sinensisaccessions (5.52–5.72 pg), whereas they were the lowest in the diploid (2n = 2x = 38)M. sacchariflorusecotypes (4.58–4.59 pg). The presented study revealed interspecific variation of nuclear DNA content (P<0.01) and therefore allowed for recognition of particular taxa, inter- and intraspecific hybrids and prediction of potential parental components. Moreover, intraspecific genome size variation (P<0.01) was observed inM. sinensiscultivars at 3.62%. The values of the stomatal size obtained for the triploidM. ×giganteus‘Great Britain’ (mean 30.70 μm) or ‘Canada’ (mean 29.67 μm) and diploidM. sinensis‘Graziella’ (mean 29.96 μm) did not differ significantly, therefore this parameter is not recommended for ploidy estimation.

Download Full-text

Further Electron Microscope Studies of a Mouse Leukemia Induced by Cell-Free Filtrates

The Journal of Cell Biology ◽

10.1083/jcb.7.4.747 ◽

1960 ◽

Vol 7 (4) ◽

pp. 747-752 ◽

Cited By ~ 57

Author(s):

Etienne de Harven ◽

Charlotte Friend

Keyword(s):

Electron Microscope ◽

Leukemia Virus ◽

Leukemic Cells ◽

Lead Salt ◽

External Diameter ◽

Intercellular Spaces ◽

Cytoplasmic Vacuoles ◽

Lead Hydroxide ◽

Cytological Characteristics ◽

Rnp Granules

Further electron microscope observations of tissues from Swiss and DBA/2 mice with leukemia transmissible by cell-free filtrates to the adult animals are presented. The cytological characteristics of the leukemic cells, the fine structure of the viruses, and the virus host-cell relationships have been examined. The leukemia virus has an external diameter averaging 870 A, and appears to be formed at the level of cell membranes by a budding process. The viruses are observed most frequently in intercellular spaces, but are also often found within cytoplasmic vacuoles of megakaryocytes. Lead hydroxide staining was applied to the study of the leukemic material. The viruses have been found to have a considerable affinity for this lead salt, only comparable in intensity to the affinity shown by RNP granules for the same chemical.

Download Full-text

Preliminary assessment of cytological characteristics of two European pear (Pyrus communis L.) cultivars

Acta Horticulturae ◽

10.17660/actahortic.2021.1315.43 ◽

2021 ◽

pp. 287-292

Author(s):

S. Kadkhodaei ◽

K. Arzani ◽

A. Yadollahi ◽

G. Karimzadeh ◽

H. Abdollahi

Keyword(s):

Pyrus Communis ◽

Preliminary Assessment ◽

European Pear ◽

Pyrus Communis L ◽

Cytological Characteristics

Download Full-text

Rosai-Dorfman Disease: Self-Resolving Unilateral Lymphadenopathy and a Brief Review of Literature

Case Reports in Oncological Medicine ◽

10.1155/2018/4869680 ◽

2018 ◽

Vol 2018 ◽

pp. 1-4

Author(s):

Joshua Feriante ◽

Richard T. Lee

Keyword(s):

The Netherlands ◽

Rare Disease ◽

Laboratory Testing ◽

Recent Literature ◽

Review Of Literature ◽

Caucasian Male ◽

Massive Lymphadenopathy ◽

Rosai Dorfman Disease ◽

Cytological Characteristics ◽

Rare Diagnosis

Rosai-Dorfman disease (RDD), also known as sinus histiocytosis with massive lymphadenopathy (SHML), is a disease of histiocytic proliferation with no known pathogenesis. This disease is defined by histological and cytological characteristics, including emperipolesis and positive S100 and CD68 markers. Although the disease typically presents clinically with massive bilateral lymphadenopathy due to sinus expansion from excessive histiocytosis, only unilateral lymphadenopathy was observed in this patient. The case involves a 40-year-old Caucasian male from the Netherlands presenting with unilateral lymphadenopathy suspicious for malignancy. Subsequent histological and laboratory testing led to the rare diagnosis of RDD. The lymphadenopathy resolved spontaneously over the course of several weeks following the initial presentation. Rosai-Dorfman disease reportedly has a benign prognosis with approximately 20% of patients experiencing spontaneous disease resolution (as was the case for this patient) with 70% experiencing chronic symptoms that may last years but not require intervention. We therefore advocate observation as a mainstay of treatment for most cases of this rare disease with intervention only being pursued in symptomatic cases. A review of recent literature regarding pathogenesis, epidemiology, diagnostic factors, prognosis, and treatment is provided and discussed.

Download Full-text