Assessing the functional and histological effect of low-level laser therapy on spinal cord compression induced injuries on Wistar rats

Background: The problem of protecting and maintaining health of workers in the microelectronics industry exposed to adverse occupational factors including tension and overstrain of the muscles of the upper extremities and visual strain, gains special relevance. Objective: Introduction of the developed and patented low-level laser therapy techniques to recover functions of vision and the musculoskeletal system impaired in the course of work. Materials and methods: The study involved 65 chip assemblers aged 26–41 years. Their eyes, the back of the hands, and the collar area were exposed to low-level laser radiation from the red region of the spectrum. All employees were examined by a neurologist and a surgeon and their working conditions were evaluated. The accommodation apparatus was tested using an AKA-01 type accommodation meter. Results: After five 10-minute sessions, we registered an increase in the volume of absolute accommodation during the observation period before and after the work shift by 28 % and 19 %, respectively. On subsequent days of observation, we noted an increase in the accommodation during 10 days, followed by a decrease by the 30th day of observation. Application of preventive techniques helped eliminate spinal cord disorders in all workers. Blood circulation in fingers increased by 66 % compared to the initial condition. Discussion: Low-level laser therapy improves blood supply, intensifies redox processes in the retina of the eye, skin cells, and blood vessels. Conclusions: Low-level laser radiation of the red region of the spectrum relieves the spasm of accommodation and normalizes the efficiency of the accommodation apparatus. Such laser therapy helps eliminate spinal cord disorders and improve blood circulation. Introduction of the developed techniques can prevent occupational diseases and reduce disability in patients.

Download Full-text

Low-level laser therapy on the rat’s gastrocnemius morphometry submitted to a rheumatoid arthritis model

European Journal of Clinical and Experimental Medicine ◽

10.15584/ejcem.2021.2.1 ◽

2021 ◽

Vol 19 (2) ◽

pp. 111-115

Author(s):

Estéfani Marin ◽

◽

Jacqueline Lumy Fuse ◽

Larissa Pereira Lopes ◽

Morgana Neves ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Laser Therapy ◽

Wistar Rats ◽

Low Level Laser Therapy ◽

Lesion Group ◽

Control Group ◽

Low Level ◽

Low Level Laser ◽

Level Laser ◽

Significant Difference

Introduction. Rheumatoid arthritis (RA) is a chronic, systemic, autoimmune inflammatory disease of unknown origin, mainly affecting synovial joints and related structures, including the adjacent musculature, generating great disability and reduction in quality of life. Aim. This study was designed to investigate the effect of low-level laser therapy (LLLT) on gastrocnemius of Wistar rats subjected to an experimental model of RA. Material and methods. Forty male Wistar rats were used, separated into: acute and chronic, being subdivided into Control Group (CG): without intervention, Lesion Group (LG): submitted to lesion, Laser Control Group (LCG): without lesion and with treatment, and Laser Lesion Group (LLG): submitted to lesion and LLLT. The treatment with LLLT occurred in four points of the right knee, wavelength of 660 nm, energy density of 5 J/cm2, energy per point of 0.003 J. Morphometric analysis was performed using a 40x magnification photomicrograph and analyzed using the Image-Pro-Plus 6.0 program. Results. As result of the acute group there was a difference only for muscle mass, being higher in CG. For the chronic group there was significant difference for cross-sectional area, larger and smaller diameter, again with the control group obtaining higher values than the others, for the number of nuclei LG was lower than CG and LCG, but LLG was not different from any of them. Conclusion. It is concluded that treatment with LLLT was not very effective in reversing the harmful effects of RA on the gastrocnemius muscle.

Download Full-text