Nanosized titanium dioxide material. Modulation of spontaneous motility and gaba-dependent regulation of functions of stomach smooth muscles in vivo

Here, we present a bone implant system of phase-oriented titanium dioxide (TiO2) fabricated by the micro-arc oxidation method (MAO) on β-Ti to facilitate improved osseointegration. This (101) rutile-phase-dominant MAO TiO2 (R-TiO2) is biocompatible due to its high surface roughness, bone-mimetic structure, and preferential crystalline orientation. Furthermore, (101) R-TiO2 possesses active and abundant hydroxyl groups that play a significant role in enhancing hydroxyapatite formation and cell adhesion and promote cell activity leading to osseointegration. The implants had been elicited their favorable cellular behavior in vitro in the previous publications; in addition, they exhibit excellent shear strength and promote bone–implant contact, osteogenesis, and tissue formation in vivo. Hence, it can be concluded that this MAO R-TiO2 bone implant system provides a favorable active surface for efficient osseointegration and is suitable for clinical applications.

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In vivo/in silico insight into the effect of titanium dioxide nanoparticle on serum paraoxonase 1 activity in rat

Journal of Biomolecular Structure and Dynamics ◽

10.1080/07391102.2020.1864662 ◽

2021 ◽

pp. 1-11

Author(s):

Hessameddin Mortazavi ◽

Hossein Omidi-Ardali ◽

Seyed Asadollah Amini ◽

Javad Saffari-Chaleshtori ◽

Keihan Ghatreh Samani

Keyword(s):

Titanium Dioxide ◽

In Silico ◽

Paraoxonase 1 ◽

Titanium Dioxide Nanoparticle ◽

Serum Paraoxonase ◽

Insight Into

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Unraveling the neurotoxicity of titanium dioxide nanoparticles: focusing on molecular mechanisms

Beilstein Journal of Nanotechnology ◽

10.3762/bjnano.7.57 ◽

2016 ◽

Vol 7 ◽

pp. 645-654 ◽

Cited By ~ 17

Author(s):

Bin Song ◽

Yanli Zhang ◽

Jia Liu ◽

Xiaoli Feng ◽

Ting Zhou ◽

...

Keyword(s):

Dna Methylation ◽

Titanium Dioxide ◽

Molecular Mechanisms ◽

Titanium Dioxide Nanoparticles ◽

Brain Dysfunction ◽

In Vivo Studies ◽

Cell Components ◽

New Perspective ◽

The Brain

Titanium dioxide nanoparticles (TiO2 NPs) possess unique characteristics and are widely used in many fields. Numerous in vivo studies, exposing experimental animals to these NPs through systematic administration, have suggested that TiO2 NPs can accumulate in the brain and induce brain dysfunction. Nevertheless, the exact mechanisms underlying the neurotoxicity of TiO2 NPs remain unclear. However, we have concluded from previous studies that these mechanisms mainly consist of oxidative stress (OS), apoptosis, inflammatory response, genotoxicity, and direct impairment of cell components. Meanwhile, other factors such as disturbed distributions of trace elements, disrupted signaling pathways, dysregulated neurotransmitters and synaptic plasticity have also been shown to contribute to neurotoxicity of TiO2 NPs. Recently, studies on autophagy and DNA methylation have shed some light on possible mechanisms of nanotoxicity. Therefore, we offer a new perspective that autophagy and DNA methylation could contribute to neurotoxicity of TiO2 NPs. Undoubtedly, more studies are needed to test this idea in the future. In short, to fully understand the health threats posed by TiO2 NPs and to improve the bio-safety of TiO2 NPs-based products, the neurotoxicity of TiO2 NPs must be investigated comprehensively through studying every possible molecular mechanism.

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BM-520, an original TXA2 modulator, inhibits the action of thromboxane A2 and 8-iso-prostaglandin F2α in vitro and in vivo on human and rodent platelets, and aortic vascular smooth muscles from rodents

Prostaglandins & Other Lipid Mediators ◽

10.1016/j.prostaglandins.2007.03.002 ◽

2007 ◽

Vol 84 (1-2) ◽

pp. 14-23 ◽

Cited By ~ 7

Author(s):

S. Rolin ◽

J. Hanson ◽

C. Vastersaegher ◽

C. Cherdon ◽

D. Pratico ◽

...

Keyword(s):

Smooth Muscles ◽

Prostaglandin F2α ◽

Thromboxane A2 ◽

Vascular Smooth Muscles

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Enhancement on Stability of Nanosized Titanium Dioxide in Silicone Suspension Using Diblock Copolymer: Influence of Dispersant Structure

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.747.599 ◽

2013 ◽

Vol 747 ◽

pp. 599-602

Author(s):

Surachet Toommee ◽

Nisanart Traiphol

Keyword(s):

Titanium Dioxide ◽

Surface Area ◽

Diblock Copolymer ◽

High Efficiency ◽

Particle Surface ◽

Ethyl Acrylate ◽

Polymer Structure ◽

Particle Agglomeration ◽

Nanosized Titanium Dioxide ◽

Enhanced Adsorption

This research investigates using of poly (dimethylsiloxane-b-hydroxy ethyl acrylate) (PDMS-b-PHEA) diblock copolymer to stabilized TiO2nanoparticles in silicone fluid. The polar PHEA segment is expected to anchor on TiO2surface while the non-polar PDMS segment extends into silicone medium. To study effects of polymer structure on its stabilizing efficiency, PDMS-b-PHEA of structures 5-b-0.3, 5-b-1.1 and 8-b-1.0 are used. Results show that suspensions of particle with surface area ~40 and ~200 m2/g can be stabilized for longer than 1 and 7 hrs, respectively. The copolymer with relatively long PHEA and PDMS segments is highly effective as a dispersant. This is due to enhanced adsorption on particle surface and steric stabilization. However, in the system of 40 m2/g-TiO2, excess amounts could lead to polymer entanglement and particle agglomeration. The copolymer dispersant exhibits high efficiency for the 200 m2/g-TiO2suspension as well. In the latter system, higher concentration is required to effectively cover particle surface.

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Aging-associated changes in microRNA expression profile of internal anal sphincter smooth muscle: Role of microRNA-133a

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00290.2016 ◽

2016 ◽

Vol 311 (5) ◽

pp. G964-G973 ◽

Cited By ~ 12

Author(s):

Jagmohan Singh ◽

Ettickan Boopathi ◽

Sankar Addya ◽

Benjamin Phillips ◽

Isidore Rigoutsos ◽

...

Keyword(s):

Smooth Muscle ◽

Anal Sphincter ◽

Internal Anal Sphincter ◽

Smooth Muscles ◽

Smooth Muscle Contractility ◽

Network Analyses ◽

Rhoa Signaling

A comprehensive genomic and proteomic, computational, and physiological approach was employed to examine the (previously unexplored) role of microRNAs (miRNAs) as regulators of internal anal sphincter (IAS) smooth muscle contractile phenotype and basal tone. miRNA profiling, genome-wide expression, validation, and network analyses were employed to assess changes in mRNA and miRNA expression in IAS smooth muscles from young vs. aging rats. Multiple miRNAs, including rno-miR-1, rno-miR-340-5p, rno-miR-185, rno-miR-199a-3p, rno-miR-200c, rno-miR-200b, rno-miR-31, rno-miR-133a, and rno-miR-206, were found to be upregulated in aging IAS. qPCR confirmed the upregulated expression of these miRNAs and downregulation of multiple, predicted targets ( Eln, Col3a1, Col1a1, Zeb2, Myocd, Srf, Smad1, Smad2, Rhoa/Rock2, Fn1, Tagln v2, Klf4, and Acta2) involved in regulation of smooth muscle contractility. Subsequent studies demonstrated an aging-associated increase in the expression of miR-133a, corresponding decreases in RhoA, ROCK2, MYOCD, SRF, and SM22α protein expression, RhoA-signaling, and a decrease in basal and agonist [U-46619 (thromboxane A2analog)]-induced increase in the IAS tone. Moreover, in vitro transfection of miR-133a caused a dose-dependent increase of IAS tone in strips, which was reversed by anti-miR-133a. Last, in vivo perianal injection of anti-miR-133a reversed the loss of IAS tone associated with age. This work establishes the important regulatory effect of miRNA-133a on basal and agonist-stimulated IAS tone. Moreover, reversal of age-associated loss of tone via anti-miR delivery strongly implicates miR dysregulation as a causal factor in the aging-associated decrease in IAS tone and suggests that miR-133a is a feasible therapeutic target in aging-associated rectoanal incontinence.

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