scholarly journals Response of isolated buccal epithelium cells on the combined action of anti-tumor antibiotic doxorubicin and magnetic field

2020 ◽  
pp. 14-23
Author(s):  
D Miroshnik ◽  
◽  
Y Shckorbatov ◽  
Keyword(s):  
Magnetic Field ◽  
Cell Membrane ◽  
Membrane Permeability ◽  
Defense Mechanisms ◽  
Protective Action ◽  
Cell Membrane Permeability ◽  
Antitumor Antibiotic ◽  
Cell Nuclei ◽  
Protective Mechanisms ◽  
The Magnetic Field

The combined effect of doxorubicin and magnetic field on the viability of isolated buccal epithelial cells two donors was investigated. Doxorubicin is an effective antitumor antibiotic, but this drug has a large amount of side effects. This significantly reduces the number of cases when you can change the drug. To reduce the toxic effect or to increase the effectiveness of anticancer therapy, the introduction of additional substances is used. For a long time, magnetic and electromagnetic fields have been used as a therapeutic factor by three therapies for cancer alone, or as an adjunct. Doxorubicin at a concentration of 2 μg/ml for 2 hours has been shown to increase of cell membrane permeability and heterochromatin granule quantity in cell nuclei. Exposure of cells to 25 mT magnetic field for 30 and 60 minutes results in decrease in cell viability and increase in cell membrane permeability and the number of heterochromatin granules in nuclei. Combined exposure to static magnetic field and doxorubicin results in decrease of the number of heterochromatin granules in the nuclei of cells from one of the donors, as compared to the variant of exposure only to doxorubicin, which indicates the protective effect of the magnetic field. A possible mechanism of the protective action of the magnetic field in our experiments is that the stress under the influence of the magnetic field activates the protective mechanisms of the cell (including the transition of chromatin to a heterochromatinized state). That way, been pre-activated due to the action of the magnetic field, the defense mechanisms of the cell reduce the toxic effects caused by doxorubicin.

Sarcolemmal permeability changes during early myocardial anoxia

1978 ◽  
Vol 36 (2) ◽  
pp. 642-643
Author(s):  
M. Ashraf ◽  
L. Landa ◽  
L. Nimmo ◽  
C. M. Bloor
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Cell Membrane Permeability ◽  
Enzyme Release ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Sarcolemmal Permeability ◽  
Membrane Changes

Following coronary artery occlusion, the myocardial cells lose intracellular enzymes that appear in the serum 3 hrs later. By this time the cells in the ischemic zone have already undergone irreversible changes, and the cell membrane permeability is variably altered in the ischemic cells. At certain stages or intervals the cell membrane changes, allowing release of cytoplasmic enzymes. To correlate the changes in cell membrane permeability with the enzyme release, we used colloidal lanthanum (La+++) as a histological permeability marker in the isolated perfused hearts. The hearts removed from sprague-Dawley rats were perfused with standard Krebs-Henseleit medium gassed with 95% O2 + 5% CO2. The hypoxic medium contained mannitol instead of dextrose and was bubbled with 95% N2 + 5% CO2. The final osmolarity of the medium was 295 M osmol, pH 7. 4.

Synthesis of novel cationic spermine-conjugated phosphotriester oligonucleotide for improvement of cell membrane permeability

2015 ◽  
Vol 25 (17) ◽  
pp. 3610-3615 ◽  
Author(s):  
Junsuke Hayashi ◽  
Tomoko Hamada ◽  
Ikumi Sasaki ◽  
Osamu Nakagawa ◽  
Shun-ichi Wada ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Cell Membrane Permeability

scholarly journals Red Cell Membrane Permeability Deduced from Bulk Diffusion Coefficients

1974 ◽  
Vol 64 (6) ◽  
pp. 706-729 ◽  
Author(s):  
W. R. Redwood ◽  
E. Rall ◽  
W. Perl
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Diffusion Coefficients ◽  
Bulk Diffusion ◽  
Red Cells ◽  
Cell Membrane Permeability ◽  
Red Cell ◽  
Cell Column ◽  
Red Cell Membrane ◽  
One Dimensional

The permeability coefficients of dog red cell membrane to tritiated water and to a series of[14C]amides have been deduced from bulk diffusion measurements through a "tissue" composed of packed red cells. Red cells were packed by centrifugation inside polyethylene tubing. The red cell column was pulsed at one end with radiolabeled solute and diffusion was allowed to proceed for several hours. The distribution of radioactivity along the red cell column was measured by sequential slicing and counting, and the diffusion coefficient was determined by a simple plotting technique, assuming a one-dimensional diffusional model. In order to derive the red cell membrane permeability coefficient from the bulk diffusion coefficient, the red cells were assumed to be packed in a regular manner approximating closely spaced parallelopipeds. The local steady-state diffusional flux was idealized as a one-dimensional intracellular pathway in parallel with a one-dimensional extracellular pathway with solute exchange occurring within the series pathway and between the pathways. The diffusion coefficients in the intracellular and extracellular pathways were estimated from bulk diffusion measurements through concentrated hemoglobin solutions and plasma, respectively; while the volume of the extracellular pathway was determined using radiolabeled sucrose. The membrane permeability coefficients were in satisfactory agreement with the data of Sha'afi, R. I., C. M. Gary-Bobo, and A. K. Solomon (1971. J. Gen. Physiol. 58:238) obtained by a rapid-reaction technique. The method is simple and particularly well suited for rapidly permeating solutes.

Non-contact-based determination of membrane permeability to water and dimethyl sulfoxide of cells virtually trapped in a self-induced micro-vortex

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Hsiu-Yang Tseng ◽  
Chiu-Jen Chen ◽  
Zong-Lin Wu ◽  
Yong-Ming Ye ◽  
Guo-Zhen Huang
Keyword(s):  
Cell Membrane ◽  
Dimethyl Sulfoxide ◽  
Membrane Permeability ◽  
Cell Membrane Permeability ◽  
Cell Type ◽  
Cryoprotective Agents ◽  
A Cell

Cell-membrane permeability to water (Lp) and cryoprotective agents (Ps) of a cell type is a crucial cellular information for achieving optimal cryopreservation in the biobanking industry. In this work, a...

scholarly journals Aluminum and Temperature Alteration of Cell Membrane Permeability of Quercus rubra

1991 ◽  
Vol 96 (2) ◽  
pp. 644-649 ◽  
Author(s):  
Junping Chen ◽  
Edward I. Sucoff ◽  
Eduard J. Stadelmann
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Quercus Rubra ◽  
Cell Membrane Permeability

THE INFLUENCE OF pH ON ALGAL CELL MEMBRANE PERMEABILITY AND ITS IMPLICATIONS FOR THE UPTAKE OF LIPOPHILIC METAL COMPLEXES1

2012 ◽  
Vol 48 (2) ◽  
pp. 293-302 ◽  
Author(s):  
Michel Lavoie ◽  
Séverine Le Faucheur ◽  
Amiel Boullemant ◽  
Claude Fortin ◽  
Peter G. C. Campbell
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Algal Cell ◽  
Cell Membrane Permeability ◽  
Influence Of Ph

scholarly journals Effect of a static magnetic field on the microscopic characteristics of highly efficient oil-removing bacteria

2017 ◽  
Vol 77 (2) ◽  
pp. 296-303 ◽  
Author(s):  
Zhijun Ren ◽  
Xiaodong Leng ◽  
Qian Liu
Keyword(s):  
Magnetic Field ◽  
Magnetic Fields ◽  
Cell Membrane ◽  
Static Magnetic Field ◽  
Cell Permeability ◽  
Cell Membrane Permeability ◽  
Oil Removal ◽  
Low Intensity ◽  
Highly Efficient ◽  
Acinetobacter Sp

Abstract To better understand the microbial oil removal enhancement process by a magnetic field, the effect of a static magnetic field (SMF) on the microscopic characteristics of highly efficient biodegradation oil-removing bacteria was studied. The Acinetobacter sp. B11 strain with a 53.6% oil removal rate was selected as the reference bacteria. The changes in the microscopic characteristics of Acinetobacter sp. B11 such as the cell surface morphology, cell permeability and cell activity of the bacteria were investigated. The results showed that low-intensity magnetic fields (15–35 mT) improved the ability of Acinetobacter sp. B11 to remove oil by 11.9% at 25 mT compared with that of bacteria with no magnetic field. Without destroying the cell membrane, the low-intensity magnetic fields increased the cell membrane permeability and improved the activity of superoxide dismutase (SOD), which effectively enhanced the oil degradation performance of the bacteria.

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