Non-contact-based determination of membrane permeability to water and dimethyl sulfoxide of cells virtually trapped in a self-induced micro-vortex

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Hsiu-Yang Tseng ◽  
Chiu-Jen Chen ◽  
Zong-Lin Wu ◽  
Yong-Ming Ye ◽  
Guo-Zhen Huang
Keyword(s):  
Cell Membrane ◽  
Dimethyl Sulfoxide ◽  
Membrane Permeability ◽  
Cell Membrane Permeability ◽  
Cell Type ◽  
Cryoprotective Agents ◽  
A Cell

Cell-membrane permeability to water (Lp) and cryoprotective agents (Ps) of a cell type is a crucial cellular information for achieving optimal cryopreservation in the biobanking industry. In this work, a...

Improvement of cell membrane permeability using a cell-solution electrode for generating atmospheric-pressure plasma

2015 ◽  
Vol 10 (2) ◽  
pp. 029521 ◽  
Author(s):  
Toshiro Kaneko ◽  
Shota Sasaki ◽  
Yutaro Hokari ◽  
Shinichi Horiuchi ◽  
Ryusuke Honda ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Cell Membrane Permeability ◽  
Pressure Plasma ◽  
A Cell

scholarly journals A highly-occupied, single-cell trapping microarray for determination of cell membrane permeability

Lab on a Chip ◽  
2017 ◽  
Vol 17 (23) ◽  
pp. 4077-4088 ◽  
Author(s):  
Lindong Weng ◽  
Felix Ellett ◽  
Jon Edd ◽  
Keith H. K. Wong ◽  
Korkut Uygun ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Single Cell ◽  
Membrane Permeability ◽  
Single Cells ◽  
Cell Membrane Permeability ◽  
Volumetric Change ◽  
Cell Trapping ◽  
Single Cell Trapping ◽  
Passive Pumping

A passive pumping, single-cell trapping microarray was developed to monitor volumetric change of multiple, single cells following hypertonic exposure.

scholarly journals Determination of cell membrane permeability in concentrated cell ensembles

1987 ◽  
Vol 52 (5) ◽  
pp. 763-774 ◽  
Author(s):  
J.A. Ochoa ◽  
S. Whitaker ◽  
P. Stroeve
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Cell Membrane Permeability

Filter-exchange PGSE NMR determination of cell membrane permeability

2009 ◽  
Vol 200 (2) ◽  
pp. 291-295 ◽  
Author(s):  
Ingrid Åslund ◽  
Agnieszka Nowacka ◽  
Markus Nilsson ◽  
Daniel Topgaard
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Cell Membrane Permeability ◽  
Pgse Nmr

scholarly journals Differential effects of cotreatment of the antibiotic rifampin with host-directed therapeutics in reducing intracellular Staphylococcus aureus infection

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10330
Author(s):  
Melissa D. Evans ◽  
Robert Sammelson ◽  
Susan McDowell
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Cell Membrane ◽  
Host Cell ◽  
Membrane Permeability ◽  
Treatment Options ◽  
Cell Membrane Permeability ◽  
Host Cell Membrane ◽  
Hek 293 ◽  
A Cell

Background Chronic infection by Staphylococcus aureus drives pathogenesis in important clinical settings, such as recurrent pulmonary infection in cystic fibrosis and relapsing infection in osteomyelitis. Treatment options for intracellular S. aureus infection are limited. Rifampin, a lipophilic antibiotic, readily penetrates host cell membranes, yet monotherapy is associated with rapid antibiotic resistance and development of severe adverse events. Antibiotic cotreatment can reduce this progression, yet efficacy diminishes as antibiotic resistance develops. ML141 and simvastatin inhibit S. aureus invasion through host-directed rather than bactericidal mechanisms. Objective To determine whether cotreatment of ML141 or of simvastatin with rifampin would enhance rifampin efficacy. Methods Assays to assess host cell invasion, host cell viability, host cell membrane permeability, and bactericidal activity were performed using the human embryonic kidney (HEK) 293-A cell line infected with S. aureus (29213) and treated with vehicle control, simvastatin, ML141, rifampin, or cotreatment of simvastatin or ML141 with rifampin. Results We found cotreatment of ML141 with rifampin reduced intracellular infection nearly 85% when compared to the no treatment control. This decrease more than doubled the average 40% reduction in response to rifampin monotherapy. In contrast, cotreatment of simvastatin with rifampin failed to improve rifampin efficacy. Also, in contrast to ML141, simvastatin increased propidium iodide (PI) positive cells, from an average of 10% in control HEK 293-A cells to nearly 20% in simvastatin-treated cells, indicating an increase in host cell membrane permeability. The simvastatin-induced increase was reversed to control levels by cotreatment of simvastatin with rifampin. Conclusion Taken together, rifampin efficacy is increased through host-directed inhibition of S. aureus invasion by ML141, while efficacy is not increased by simvastatin. Considerations regarding novel therapeutic approaches may be dependent on underlying differences in pharmacology.

Sarcolemmal permeability changes during early myocardial anoxia

1978 ◽  
Vol 36 (2) ◽  
pp. 642-643
Author(s):  
M. Ashraf ◽  
L. Landa ◽  
L. Nimmo ◽  
C. M. Bloor
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Cell Membrane Permeability ◽  
Enzyme Release ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Sarcolemmal Permeability ◽  
Membrane Changes

Following coronary artery occlusion, the myocardial cells lose intracellular enzymes that appear in the serum 3 hrs later. By this time the cells in the ischemic zone have already undergone irreversible changes, and the cell membrane permeability is variably altered in the ischemic cells. At certain stages or intervals the cell membrane changes, allowing release of cytoplasmic enzymes. To correlate the changes in cell membrane permeability with the enzyme release, we used colloidal lanthanum (La+++) as a histological permeability marker in the isolated perfused hearts. The hearts removed from sprague-Dawley rats were perfused with standard Krebs-Henseleit medium gassed with 95% O2 + 5% CO2. The hypoxic medium contained mannitol instead of dextrose and was bubbled with 95% N2 + 5% CO2. The final osmolarity of the medium was 295 M osmol, pH 7. 4.

Bacterial translocation, intestinal ultrastructure and cell membrane permeability early after major liver resection in the rat

1994 ◽  
Vol 81 (4) ◽  
pp. 579-584 ◽  
Author(s):  
X. D. Wang ◽  
H. Pärsson ◽  
R. Andersson ◽  
V. Soltesz ◽  
K. Johansson ◽  
...  
Keyword(s):  
Liver Resection ◽  
Cell Membrane ◽  
Membrane Permeability ◽  
Bacterial Translocation ◽  
Cell Membrane Permeability ◽  
Major Liver Resection
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