scholarly journals Genetic analysis of yield and heat stress related traits in wheat (Triticum aestivum L. em. Thell) using microsatellite markers

2015 ◽  
Vol 7 (2) ◽  
pp. 739-744 ◽  
Author(s):  
Mamta Gupta ◽  
Veena Chawla ◽  
Pankaj Garg ◽  
Neelam Yadav ◽  
Renu Munjal ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Heat Stress ◽  
Genetic Analysis ◽  
Microsatellite Markers ◽  
Threshold Value ◽  
Triticum Aestivum L ◽  
Interval Mapping ◽  
Single Marker Analysis ◽  
D Genome ◽  
A Genome

Microsatellite markers were used for genetic analysis of terminal heat tolerance in F2 (PBW373 × WH1081) population of wheat (Triticum aestivum L. em. Thell). Two parents were evaluated in field under normal sown and late sown conditions. For genotyping DNA from both parents PBW373 and WH1081 was amplified using 200 SSRs. Only 22 SSRs produced polymorphic bands, of size between 100 to 300 bp and an average of 1.45 alleles. The single marker analysis identified 19 markers indicating the putative QTLs for yield, its components and heat stress related physiological traits. The number of markers on these 16 linkage groups varied from one to four. On A genome 13 QTLs on B genome 5 QTLs and on D genome 9 QTLs were identified, respectively. The A, B and D genomes had 1360.3 cM, 272.4 cM and 919.5 cM of linkage coverage with average interval distances of 104.63 cM, 54.48 cM and 102.16 cM/Marker. A total of nine QTLs were resolved following composite interval mapping, one QTL was detected at a LOD score equal to threshold value of 2.5 while eight at LOD scores above the threshold value. All the nine QTLs were shown to be on definitive location on chromosome 3A (QDh.CCSHAU-3A, QDa.CCSHAU-3A and QPm.CCSHAU-3A), chromosome (QBm.CCSHAU-5A, QCtd.CCSHAU-5A and QCl.fl.CCSHAU-5A), chromosome6A (QPh.CCSHAU-6A) and chromosome3B (QTgw.CCSHAU and QMts.CCSHAU-3B). Use of these markers save times, resources and energy that are needed not only for raising large segregating populations for sveral generations, but also for estimating the parameters used for selection.

GENETIC REGULATION OF HETEROGENETIC CHROMOSOME PAIRING IN POLYPLOID SPECIES OF THE GENUS TRITICUM sensu lato

1982 ◽  
Vol 24 (1) ◽  
pp. 57-82 ◽  
Author(s):  
Patrick E. McGuire ◽  
Jan Dvořák
Keyword(s):  
Triticum Aestivum ◽  
Chromosome Pairing ◽  
Chinese Spring ◽  
Genetic Regulation ◽  
Triticum Aestivum L ◽  
Polyploid Species ◽  
D Genome ◽  
Chromosome 5B ◽  
A Genome

Polyploid species of Triticum sensu lato were crossed with Triticum aestivum L. em. Thell. cv. Chinese Spring monotelodisomics or ditelosomics that were monosomic for chromosome 5B. Progeny from these crosses were either euploid, nullisomic for 5B, monotelosomic for a given Chinese Spring chromosome, or nullisomic for 5B and monotelosomic simultaneously. The Chinese Spring telosome in the hybrids permitted the evaluation of autosyndesis of chromosomes of the tested species. In addition, several Chinese Spring eu- and aneuhaploids were produced. Genotypes of T. cylindricum Ces., T. juvenale Thell., T. triunciale (L.) Raspail, T. ovatum (L.) Raspail, T. columnare (Zhuk.) Morris et Sears, T. triaristatum (Willd.) Godr. et Gren., and T. rectum (Zhuk.) comb. nov. were all shown to have suppressive effects on heterogenetic pairing in hybrids lacking 5B or 3AS, whereas T. kotschyi (Boiss.) Bowden had no effect. It was concluded that diploid-like meiosis in these species is due to genetic regulation. A number of these genotypes promoted heterogenetic pairing in the presence of 5B. A model is presented to explain this dichotomous behavior of the tested genotypes. Monotelosomic-3AL haploids had a greater amount of pairing than did euhaploid Chinese Spring, which substantiated the presence of a pairing suppressor(s) on the 3AS arm. Evidence is presented that shows that T. juvenale does not have a genome homologous with the D genome of T. aestivum.

scholarly journals A Microsatellite Map of Wheat

Genetics ◽  
1998 ◽  
Vol 149 (4) ◽  
pp. 2007-2023 ◽  
Author(s):  
Marion S Röder ◽  
Victor Korzun ◽  
Katja Wendehake ◽  
Jens Plaschke ◽  
Marie-Hélène Tixier ◽  
...  
Keyword(s):  
Bread Wheat ◽  
Reference Population ◽  
Triticum Aestivum L ◽  
Wheat Genome ◽  
D Genome ◽  
B Genome ◽  
Microsatellite Map ◽  
A Genome ◽  
Polymorphic Microsatellite ◽  
Primer Sets

Abstract Hexaploid bread wheat (Triticum aestivum L. em. Thell) is one of the world's most important crop plants and displays a very low level of intraspecific polymorphism. We report the development of highly polymorphic microsatellite markers using procedures optimized for the large wheat genome. The isolation of microsatellite-containing clones from hypomethylated regions of the wheat genome increased the proportion of useful markers almost twofold. The majority (80%) of primer sets developed are genome-specific and detect only a single locus in one of the three genomes of bread wheat (A, B, or D). Only 20% of the markers detect more than one locus. A total of 279 loci amplified by 230 primer sets were placed onto a genetic framework map composed of RFLPs previously mapped in the reference population of the International Triticeae Mapping Initiative (ITMI) Opata 85 × W7984. Sixty-five microsatellites were mapped at a LOD >2.5, and 214 microsatellites were assigned to the most likely intervals. Ninety-three loci were mapped to the A genome, 115 to the B genome, and 71 to the D genome. The markers are randomly distributed along the linkage map, with clustering in several centromeric regions.

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