scholarly journals Effect of different growth regulators on in vitro micro-propagation of Kufri Frysona

2016 ◽  
Vol 8 (2) ◽  
pp. 535-540
Author(s):  
Priyadarshani P. Mohapatra ◽  
V.K. Batra ◽  
Subhash Kajla ◽  
Anil K. Poonia ◽  
N. Manoj Kumar
Keyword(s):  
Growth Regulators ◽  
Large Scale ◽  
Basal Medium ◽  
Potato Cultivar ◽  
Shoot Tips ◽  
Scale Production ◽  
Survival Percentage ◽  
Planting Material ◽  
Micro Propagation

In the present investigation, experiment was conducted for in vitro micro-propagation with different concentration of growth regulators in different explants Sprouts and Shoot tips of potato cultivar Kufri Frysona. The maximum survival percentage (40) of sprouts and (100%) of shoot tips were obtained when the explants were surface sterilized with 0.2% bavistin & 0.4% streptocyclin (45minutes) and 0.1% mercuric chloride (60seconds). Sterilized explants were inoculated on MS basal supplemented with various growth regulators and established successfully. The maximum shoot induction (62.5±1.44%) in 11.3±0.33 days and (74.0 ± 2.13 %) in 10.0 ± 0.50 days were reported on medium PM1 (BAP 0.25 mg/l) in sprouts and shoot tip explants respectively. The sprouted explants were further sub-cultured on MS media supplemented with various growth regulator alone and in combination for in vitro multiplication. In Kufri Frysona (11.2) shoots were obtained on MS medium fortified with 0.25mg/l BAP + 0.01mg/l IAA on 42th day of subculture. In vitro rooting was observed on MS basal medium supplemented with 2.0 mg/l NAA in Kufri Frysona after 10 days. Rooted plantlets were successfully hardened in green house using different types of potting mixture and finally transferred to field. The protocol will be very useful for large-scale production of disease free planting material of potato (S. tuberosum) in future.

scholarly journals In Vitro Micropropagation of the Medicinal Plant Physalis angulata L.

2016 ◽  
Vol 8 (2) ◽  
pp. 161-163
Author(s):  
Owk ANIEL KUMAR ◽  
Songa RAMESH ◽  
Sape SUBBA TATA
Keyword(s):  
Large Scale ◽  
Leaf Disc ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Root Induction ◽  
Survival Percentage ◽  
Physalis Angulata

Physalis angulata L. is an important medicinal herb. An efficient direct adventitious plant regeneration protocol was developed for large scale propagation using leaf disc as explants. The explants were cultured on MS basal medium supplemented with 0.25-3.0 mg/L 6-benzyl amino purine (BAP) for primary shoot proliferation. Inclusion of indole-3-acetic acid (IAA) and gibberellic acid (GA3) in the culture medium along with BAP promoted a higher rate of shoot multiplication. The maximum number of shoots was produced in MS + BAP (1.0 mg/L) + IAA (0.5 mg/L) + GA3 (0.20 mg/L) after the third subculture. An average of 152.8 ± 0.40 shoots were produced from each leaf disc. For root induction the shootlets were transferred to MS medium supplemented with different concentrations of indole-3-butyric acid (IBA). The highest percentage of root induction was observed in 1.0 mg/L (IBA). Rooted plants were successfully established in the soil after hardening. The survival percentage of rooted plants on soil was found to be 85%. This result will facilitate the conservation and propagation of the important medicinal herb Physalis angulata L.

scholarly journals In vitro multiplication protocol for Curcuma mangga : Studies on carbon, cytokinin source and explant size

2021 ◽  
Vol 16 (1) ◽  
pp. 69-76
Author(s):  
A A Waman ◽  
P Bohra ◽  
R Karthika Devi ◽  
J Pixy
Keyword(s):  
Carbon Source ◽  
Large Scale ◽  
Shoot Proliferation ◽  
Ex Vitro Rooting ◽  
Tropical Species ◽  
Scale Production ◽  
Ex Vitro ◽  
In Vitro Multiplication ◽  
Planting Material

Mango ginger (Curcuma mangga Valeton & Zijp.) is an underutilized rhizomatous species that has been valued in tropical Asian countries as a source of vegetable, spice, salad, medicine, and essential oil. This species is hardy and requires less care for obtaining good yields. Rhizomes are the commonly used propagules for the species, which are also the economic part of the crop. Huge quantity of seed rhizomes is required to promote this crop in larger areas. An efficient in vitro multiplication protocol is one of the options to meet the planting material requirement. Effects of carbon source (glucose, fructose and sucrose) and concentration (1 and 3%, w/v), cytokinins (BAP and meta topolin) and concentration (1 mg/L and 2 mg/L), size of explants (one/ two/ three bud) and IBA treatment (0, 250, 500 and 1,000 mg/L) for concurrent ex vitro rooting cum hardening were studied. Results revealed that for facilitating efficient multiplication, the medium should be supplemented with glucose (3%) as a carbon source and meta topolin (1 mg/L) as cytokinin. Two-bud explant should be used for subculture as it promoted superior shoot proliferation. Concurrent ex vitro rooting cum hardening was possible even without auxin treatment. The present protocol could be useful for large-scale production of quality planting material of this underexploited tropical species.

scholarly journals CALLUS INDUCTION IN SOMATIC TISSUES OF PRUNUS PERSICA L.

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 698c-698
Author(s):  
Veronique Declerck ◽  
Schuyler S. Korban
Keyword(s):  
Growth Regulators ◽  
Large Scale ◽  
Prunus Persica ◽  
Carbon Sources ◽  
Leaf Explants ◽  
Basal Medium ◽  
In Vitro Shoots ◽  
Somatic Tissues ◽  
Half Strength

Leaf segments of Prunus persica L. (peach) collected from greenhouse-grown plants and from micropropagated shoots were cultured on a basal medium containing half-strength Murashige and Skoog (MS), Staba vitamins, sucrose (30 g/1) and agar (6.5 g/l); medium adjusted to pH 5.6. The influence of 6 different growth regulators at 3 concentrations (5, 10, 15 μM) were investigated using leaf explants from proliferating shoots of 'Elberta Queen' peach. With thidiazuron (TDZ), compact and multiple green calli were obtained; with benzyladenine and zeatin, lower numbers of small sized calli were obtained; with kinetin, no callus development was observed. Among auxin treatments, both Dicamba and 2,4-D resulted in friable white and yellow calli. Most of the calli produced in all treatments were formed along the cut margins of the explants. In an another experiment, leaf explants of' Bellaire' (greenhouse) and `Elberta Queen' (in vitro shoots) were used to determine the influence of a large scale concentration of TDZ (3 to 23 |iM). Explants from greenhouse and in vitro leaves resulted in higher levels of callus development at TDZ concentrations of 8-13 μM. Higher TDZ levels resulted in necrosis of leaf explants. The-influence of different carbon sources on callogenesis was investigated. We observed more green and compact calli with glucose than with sucrose and fructose at 100 mM. The influence of the glucose at 10 different concentrations (30 to 300 mM) was also investigated.

scholarly journals CLONAL MICROPROPAGATION OF MALE-STERILE ZINNIA ELEGANS

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1124F-1124
Author(s):  
R.B. Rogers ◽  
M.A.L. Smith ◽  
R. Cowen
Keyword(s):  
Large Scale ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Zinnia Elegans ◽  
Scale Production ◽  
Male Sterile ◽  
Clonal Micropropagation ◽  
Large Scale Production ◽  
High Humidity Environment

The only method for large scale production of pure hybrid seed in Zinnia elegans involves the use of male sterile individuals. The male sterile trait, however, is a three gene recessive which at best produces only 50% male sterile progeny from seed. Since no method of clonal propagation is available, seed-produced female lines require labor intensive field roguing to insure removal of all normal flowered individuals. Clonal micropropagation was investigated as a means of mass producing male steriles for use as female lines. Sterilization procedures were developed for seed and axillary bud explants. Shoot proliferation media containing various levels of BAP, 2ip, and kinetin were screened using in vitro germinated seedling explants of the inbred line `Orange Starlight'. Microshoots demonstrated a high rooting percentage after 2 weeks on basal medium without growth regulators. Plantlets were easily acclimated in 1 to 2 weeks in a high humidity environment. In vitro derived plants of identified male sterile plants were phenotypically evaluated as to their suitability for use in field production.

scholarly journals Biochemical assessment of in vitro and in vivo culture of Tylophora indica (Burm. F.) Merr

1970 ◽  
Vol 6 (2) ◽  
pp. 1-5
Author(s):  
Antaryami Kaushik ◽  
Chandra Gurnani ◽  
Shyam Sunder ◽  
Abha Dhingra ◽  
Vikram Chimpa
Keyword(s):  
Large Scale ◽  
Basal Medium ◽  
Nodal Segments ◽  
Scale Production ◽  
Tylophora Indica ◽  
Shoot Initiation ◽  
Large Scale Production ◽  
Chlorophyll Protein

Tylophora indica (Burm. F.) Merr is an endangered plant which can be protected from extinction by its large scale production. Nodal segments of healthy plants are used as explants and cultured on MS Basal medium fortified with different growth regulators. Optimum shoot induction conditions from explants were established. In vitro and in vivo phytochemical test were done by using standard methods for chlorophyll, carbohydrates, proteins, lipids and starch. 3mg/l 2, 4 D showed maximum and success full callus production. Shoot initiation started in 7 days and best shoot regeneration reported with 3 mg/ml BAP in Basal medium. Combination of IBA and NAA in concentration 2 and 4 mg/l respectively proved to be best for root initiation. Concentration of chlorophyll, protein, lipid, carbohydrate, and starch in vitro and in vivo culture are investigated. DOI: 10.3126/kuset.v6i2.4005Kathmandu University Journal of Science, Engineering and Technology Vol.6. No II, November, 2010, pp.1-5

scholarly journals A competent protocol for large scale production of sugarcane (Saccharum officinarum L.) through meristem culture

2016 ◽  
Vol 8 (1) ◽  
pp. 128-132 ◽  
Author(s):  
J. Udhutha ◽  
S. C. Mali ◽  
H. A. Sahare
Keyword(s):  
Apical Meristem ◽  
Large Scale ◽  
Shoot Formation ◽  
Saccharum Officinarum ◽  
Meristem Culture ◽  
Scale Production ◽  
Ms Medium ◽  
Large Scale Production ◽  
Micro Propagation

A rapid micro propagation and acclimatization response of two different varieties of sugarcane Co86032 and CoN 04131(Saccharum officinarum L.) was obtained in this study. The shoot apical meristem of different sizes wascultured on Murashige and Skoog medium supplemented with different concentrations and combinations of ben-zylaminopurine and kinetin either alone or in combination with each other alongwith GA3. Best shoot formation response in Co 86032 was obtained on MS medium containing 1.5mg/l BAP while in CoN 04131 the combination of 0.5 mg/l BAP with 0.25 mg/l Kinetin showed best shoot formation response from apical meristem. Meristem of 3.0 mm size proved to be the best size for micropropagation of sugarcane. Excellent multiplication response of In vitro formed shoots was obtained when the concentration of BAP was decreased to 1.0 mg/l in Co 86032and 0.25 mg/l BAP and Kin in CoN 04131 (i.e. 0.25 mg/lBAP + 0.25 mg/l Kinetin. MS medium containing 1.0 mg/l NAA and 2.0 mg/l IBA showed 100% rooting response of In vitro regenerated shoots of both the varieties of sugarcane within eight days of inoculation. Best hardening response was obtained in sand+ soil + pressmud (1:1:1) media.

scholarly journals Micropropagation of Homalomena pineodora Sulaiman & Boyce (Araceae): a new species from Malaysia

2012 ◽  
Vol 30 (1) ◽  
pp. 39-43 ◽  
Author(s):  
Christine Stanly ◽  
Arvind Bhatt ◽  
Baharuddin Sulaiman ◽  
Chan Lai Keng
Keyword(s):  
Large Scale ◽  
Ornamental Plants ◽  
Basal Medium ◽  
Fluorescent Light ◽  
Scale Production ◽  
Shoot Cultures ◽  
Ms Medium ◽  
Plantlet Production ◽  
Mother Plants

Homalomena pineodora (family Araceae) is a species found to have impressive foliage characteristics which remain evergreen throughout the year. Therefore, H. pineodora can be grown as an ornamental plant. Generally H. pineodora needs 3-5 years to propagate and multiply. However, the demand for new ornamental plants is increasing worldwide and the quality of planting material is a basic need for boosting productivity. Therefore an efficient micropropagation protocol for large-scale production of H. pineodora was developed. In vitro shoot cultures were initiated from the rhizomatous buds on MS basal medium. The best conditions for propagating H. pineodora was found to be MS medium supplemented with 3% sucrose and 0.5 mg L-1 BA (6-benzyladenine) under 24 h of cool fluorescent light which produced an average of 3.8 shoot per explant. Presence of an auxin was not necessary for plantlet production. Liquid MS medium supplemented with 0.5 mg L-1 BA, enhanced the shoot production of H. pineodora as compared to agar-gelled medium with same composition. All the in vitro plantlets of H. pineodora were successfully acclimatized with 100% survival rate. Scanning electron microscopy confirmed the similarity of leaf microstructures between the in vitro and mother plants of H. pineodora.

scholarly journals High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in Diplocyclos palmatus (L.) C. Jeffrey

Agronomy ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2164
Author(s):  
Anamica Upadhyay ◽  
Anwar Shahzad ◽  
Zishan Ahmad ◽  
Abdulrahman A. Alatar ◽  
Gea Guerriero ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
In Vitro Propagation ◽  
Large Scale ◽  
Basal Medium ◽  
Wild Plants ◽  
Direct Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Scale Production ◽  
Large Scale Production

Diplocyclos palmatus (L.) C. Jeffrey, commonly referred to as “Shivalingi” or “Lollipop climber” is a valuable medicinal plant with a climbing growth habit used in traditional medicine. It is reputed to have antiarthritic, anti-diabetic properties and to be useful in various skin and reproductive problems. Overexploitation of wild plants and low seed germination have resulted in the decline of the species in the wild. Thus, the present investigation was aimed to establish an effective in vitro propagation procedure for its large-scale production and conservation. Nodal explants, obtained from an established mother plant were grown on MS basal medium augmented with various cytokinins, alone or in combination with auxins, to study the morphogenic response. A maximum of 8.3 shoots/explants with an average shoot length of 7.2 cm were produced after six weeks on MS containing benzylaminopurine 5.0 µM + 1-naphthaleneacetic acid 2.0 µM. After 4 weeks of transfer, microshoots rooted well on a low nutrient medium of ½ MS + 1.0 µM indole-3-butyric acid, with a maximum of 11.0 roots/microshoot and an average root length of 7.4 cm. With an 80% survival rate, the regenerated plantlets were effectively acclimatized to natural conditions. DNA-based molecular markers were used to investigate the genetic uniformity. Scanning Electron Microscopic examination of leaves indicated the adaptation of the plantlets to natural, as evidenced by the formation of normal stomata. Gas chromatography-mass spectrometry analyses of mother and micropropagated plants were performed to identify essential secondary metabolites. The results obtained show that the in vitro propagation system can be adopted for preservation, large-scale production and secondary metabolites’ production in D. palmatus.

scholarly journals DEVELOPMENT OF IN VITRO PLANT REGENERATION PROTOCOL OF BANANA (MUSA SP.) CV. GRAND NAINE USING SUCKER EXPLANT

2021 ◽  
Vol 21 (2) ◽  
Author(s):  
Kumari Monalisa ◽  
Bibekananda Kulhari ◽  
Subhashree S. Barik ◽  
Swaraj K. Babu ◽  
Mamta Naik ◽  
...  
Keyword(s):  
Large Scale ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Multiple Shoot ◽  
Scale Production ◽  
Ms Medium ◽  
Musa Sp ◽  
Regenerated Plants ◽  
Large Scale Production

Banana is an important fruit crop belongs to the family Musaceae. This has more demand for it multifarious uses like food, medicinal as well as industrial values. The present study was carried out to develop micropropagation protocol for large scale production of banana cv. Grand naine using sucker explant. Sucker explants were inoculated on Murashige and Skoog’s (1962) (MS) basal medium and MS basal medium supplemented with different types and concentrations and combination of plant growth regulators. Highest mean number of shoots (10.2) per explant having mean shoot length 5.2 cm was observed on MS medium supplemented with 4.0 mg/L BA, 2.0 mg/L Z, 1.0 mg/L NAA, and 3.0 mg/L ADS. For large scale production of shoot, in vitro regenerated shoots were harvested, cut into small pieces and inoculated on the optimum medium for multiple shoot proliferation. In this way, more than thousand numbers of in vitro shoots were regenerated from a single explant at six month of culture. In vitro regenerated shoots were excised and rooted on ½ MS medium supplemented with 1.0 mg/L IBA. Finally in vitro regenerated plants were acclimatized and subsequently transferred to field with zero mortality. This protocol helps to meet the demand of the farmers.

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