Identification of the allelic state of the Lr34 leaf rust resistance gene in soft winter wheat cultivars developed in Ukraine

2011 ◽  
Vol 45 (5) ◽  
pp. 271-276 ◽  
Author(s):  
A. V. Karelov ◽  
Ya. V. Pirko ◽  
N. A. Kozub ◽  
I. A. Sozinov ◽  
N. N. Pirko ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Leaf Rust Resistance Gene ◽  
Wheat Cultivars ◽  
Allelic State ◽  
Winter Wheat Cultivars ◽  
Soft Winter Wheat

scholarly journals Map-Based Cloning of Leaf Rust Resistance Gene Lr21 From the Large and Polyploid Genome of Bread Wheat

Genetics ◽  
2003 ◽  
Vol 164 (2) ◽  
pp. 655-664 ◽  
Author(s):  
Li Huang ◽  
Steven A Brooks ◽  
Wanlong Li ◽  
John P Fellers ◽  
Harold N Trick ◽  
...  
Keyword(s):  
Amino Acid ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Bread Wheat ◽  
Rust Resistance ◽  
Agronomic Traits ◽  
Leaf Rust Resistance ◽  
Triticum Aestivum L ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene

Abstract We report the map-based cloning of the leaf rust resistance gene Lr21, previously mapped to a generich region at the distal end of chromosome arm 1DS of bread wheat (Triticum aestivum L.). Molecular cloning of Lr21 was facilitated by diploid/polyploid shuttle mapping strategy. Cloning of Lr21 was confirmed by genetic transformation and by a stably inherited resistance phenotype in transgenic plants. Lr21 spans 4318 bp and encodes a 1080-amino-acid protein containing a conserved nucleotide-binding site (NBS) domain, 13 imperfect leucine-rich repeats (LRRs), and a unique 151-amino-acid sequence missing from known NBS-LRR proteins at the N terminus. Fine-structure genetic analysis at the Lr21 locus detected a noncrossover (recombination without exchange of flanking markers) within a 1415-bp region resulting from either a gene conversion tract of at least 191 bp or a double crossover. The successful map-based cloning approach as demonstrated here now opens the door for cloning of many crop-specific agronomic traits located in the gene-rich regions of bread wheat.

Sign in / Sign up

Export Citation Format

Share Document