The need for the vernalization duration of soft winter wheat collection samples

The need for vernalization is a duration-dependent effect of low, positive temperatures in order to ensure the plants' transition to generative development. If the requirement for the duration of germination is not met, the plant will not enter the stage of forming generative organs. The vernalization requirements of winter soft wheat samples of different geographical origins are determined. An assessment of the vernalization period duration influence on the severity of the elements of the yield structure is given. The research material consisted of 15 cultivars of soft winter wheat of various geographic origin. The samples were germinated in paper rolls, then vernalized in a climatic chamber at a temperature of 3–5 ºС for 60, 50, and 40 days. At the end of vernalization, 10 plants of each sample were planted in a greenhouse. The dates of the onset of phenological phases were noted: tube emergence, earing, flowering. To determine the main elements of the yield structure, a structural analysis of plants was carried out. With an increase in the vernalization period, a decrease in the interfacial periods from tube emergence to flowering was noted. The influence of the timing of vernalization was noted on the manifestation of the spike length trait. It was found that the total number of stems and the number of productive stems in almost all varieties decreases with an increase in the period of vernalization. Significant differences between collection varieties in the need for vernalization, due to both their geographical origin and the genotype of plants are revealed. In all the studied forms, with an increase in the period of vernalization, the rate of plant development increased to varying degrees, the total number of stems, the productive stem and the length of the spike decreased.

Physico-chemical properties of grain of different ripening soft winter wheat varieties under different fertilizer systems

Aims. To determine the formation of physico-chemical properties of different ripening varieties of soft winter wheat grain under different fertilizer systems in a field crop rotation. Methods. Laboratory, mathematical and statistical, physicochemical. Results. The article presents the formation of physico-chemical properties (thousand grain weight, grain unit, hardness index, protein content) of different ripening varieties of soft winter wheat grain under different fertilizer systems in the field crop rotation. On average, over two years of research, thousand grain weight of KWS Emil soft winter wheat increased from 37.2 to 38.5 g, depending on the fertilizer system. The use of all types of fertilizers increased it by 3 % compared to areas without fertilizers. There is no significant difference between fertilizer application systems in the field crop rotation. The high stability index (0.96–1.00) of thousand grain weight formation under growing both varieties indicates a slight influence of weather conditions of the growing season. Thus, in 2020 this figure increased from 37.1 to 38.3 g, and in 2021 – from 37.2 to 39.1 g. In Rino variety, thousand grain weight was significantly higher than in KWS Emil variety by 16–17 %. The application of 75 kg/ha of nitrogen fertilizers increased it from 43.2 g to 43.6–43.8 g or only by 1 %, and at a double dose – to 44.8–45.2 g, or by 4–5 %. The efficiency of phosphorus-potassium fertilizers was higher than the cultivation of KWS Emil, and lower in Rino. The grain unit of both soft winter wheat varieties increased both on average and over the years of research with a stability index of 0.98–1.00. Thus, on average over two years of research, it increased in KWS Emil variety from 771 to 779–789 g/l or 1–2 % depending on the fertilizer system. Rino grain had 6% more grain unit compared to KWS Emil. On average, it increased from 820 to 831–838 g/l per fertilizer application or only by 1–2 %. Conclusions. It is established that the physico-chemical properties of grain change depending on the fertilizer system, variety and weather conditions of the growing season. Rino grain has higher physico-chemical properties. Thus, thousand grain weight increases from 43.2 to 43.6–45.2 g, grain unit – from 820 to 831–838 g/l, protein content – from 14.4 to 15.4–16.4 %, depending on the fertilizer system, hardness type – hard-grained. In KWS Emil variety, thousand grain weight increases from 37.2 to 38.3–38.5 g, grain unit – from 771 to 779–789 g/l, protein content – from 11.7 to 12.3–13.7 % depending on the fertilizer system, hardness type – soft-grained.

Transgressive variability in the number of ears of the main spike in F2 populations when crossing soft winter wheat varieties of different early maturity.

Aim. Determination of the degree and frequency of positive transgressions by the number of ears of the main spike in F2 populations created by crossing varieties of soft winter wheat of different maturity depending on the hydrothermal conditions of the year and selection of economically valuable biotypes for subsequent breeding work. Methods. Field, laboratory, mathematical and statistical. Results. In the majority of F2 populations, created by hybridization of soft winter wheat varieties of different vegetation periods, a significant form-making process took place in terms of the number of spikelets in the main spike. The experimental data obtained indicate a significant effect of matched pairs before hybridization and hydrothermal conditions of the year on the manifestation and formation of the number of spikelet’s in an ear F2. So, under the conditions of 2019, the majority of F2 populations for the number of spikelet’s in the main spike (17.4–21.5 pcs.) Significantly exceeded the parental forms. A positive degree (Td = 5.3–33.3 %) and the frequency of transgression (Tf = 3.3–96.7 %) were found in 85.7 % of the populations. In 2020, the average population number of spikelets in the main spike (15.0–18.6 pcs.) Was significantly less. When used in hybridization of early maturing varieties by the parent form, the decrease in the number of spikelets was 0.5–19.9 %. At the same time, for crossing mid-early, mid-ripening and mid-late varieties, the number of spikelets decreased by 2.8–28.9 %. Thus, it can be argued that populations in which early maturing varieties were the maternal form are less exposed to unfavorable conditions of the year. A positive degree (Td = 5.3–38.9 %) and the frequency of recombinants (Tf = 3.3–46.7 %), under the conditions of 2020, was established in 35 out of 42 F2 populations, which amounted to 83.3 %. Conclusions. The use of winter wheat varieties of different early maturity in hybridization in most cases contributes to the formation of F2 in populations and the selection of positive transgressive biotypes by the number of spikelets in the main spike. Conducted studies have established a significant effect of the parental forms used in hybridization and the hydrothermal conditions of the year on the formation of the number of spikelets in the main spike in F2 populations of soft winter wheat. Populations where the early maturing varieties were the maternal form are characterized by a more stable manifestation of the number of spikelets in the main spike. The decrease in the number of spikelets in 2020 compared to 2019 was 0.5–19.9 % for 2.8–28.9 % in populations obtained by hybridization of mid-early, mid-ripening and mid-late varieties. In years with contrasting hydrothermal conditions, hybrid populations F2 were identified, which formed more than the average F2 number of spikelets in the main spike and had high rates of the degree and frequency of positive transgressions, namely: Mironovskaya early / Kolchuga; Kolchuga / Stolichnaya; Shchedraia niva / Vidrada; Antonovka / Stolichnaya.

Increasing photosynthetic productivity of crops as affected by water content in their leaves under mycorrhization of the root system

Purpose. To investigate the effect of pre-sowing seed treatment with vesicular-arbuscular mycorrhizal (VAM) and bacterial preparations on the leaf water content and photosynthetic productivity of agricultural crops (winter wheat, corn, sunflower and soybean) under mycorrhization of their roots. Methods. Field, laboratory, and statistical. Results. The results of studies on determining the effect of pre-sowing treatment of seeds of such agricultural crops as soft winter wheat, common corn, annual sunflower and soybean culturally vesucular-arboscular drugs Mikovital (mushroom Tuber melanosporum VITTAD.) Micofrend (mushroom Trichoderma harzianum. (bacteria Bacillus subtilis Cohn.) on the water content of leaves and the productivity of photosynthesis of plants by mycorrhization of their root system. It was found that the implementation of this measure provides a steady increase in the water content of leaves and the productivity of photosynthesis of agricultural crops. In particular, the water content of leaves of soft winter wheat plants, common corn, annual sunflower and cultivated soybeans for the use of the preparations Mikovital, Mikofrend and Florobatsilin was 1.6–77.3 % more than in the control. According to the indicators of the water content of the leaves, the productivity of photosynthesis of crops increased by 8.3–57.1 % in comparison with the control, which indicates a significant effect of this factor on the growth and development of plants. Conclusions. Pre-sowing seed treatment with vesicular-arbuscular products Mikovital, Mycofrend, and bacterial product Florabacillin promotes a steady increase in the leaf water content and photosynthetic productivity in winter bread wheat, maize, annual sunflower, and soybean.

Razdolye – a new mid-late variety of soft winter wheat

Wheat bread rightfully belongs to the greatest inventions of mankind. The aim of the research is to characterize the new Razdolye soft winter wheat variety for compliance with these requirements. The research was carried out in 2016-2020. The research object was the Razdolye winter wheat variety. The Ermak variety was used as a standard. The accounting area of the plot is 10 m2. The Razdolye variety was submitted to the State Variety Testing of the ARC “Donskoy” jointly with the Federal State Budgetary Organisation “Research Institute of Agriculture of Crimea”. Razdolye has a high grain yield. The average yield of Razdolye in competitive trials for the predecessor green manure fallow over five years of study (2016-2020) was 10.57 t/ha, which is 1.68 t/ha higher than the standard variety Ermak. By ripening, it belongs to the middle late ripeness group, hatches and ripens 4 days later than the standard variety. Possesses high drought resistance, heat resistance, frost resistance and resistance to the main diseases of the region. Razdolye is being studied since the fall of 2020 in the North Caucasus, Lower Volga and Central Black Earth regions of the Russian Federation.

Weather Factors and Their Influence on the Adaptive Properties of Winter Wheat Varieties in the Western Forest-Steppe of Ukraine

The yield potential of winter wheat (Triticum aestivum L.) is formed in changing weather conditions and depends on the proposed agro-technological measures, to which the response of a particular variety is different. The purpose of this study was to determine the influence of weather factors on the field germination of soft winter wheat seeds, the growth and development of plants in the autumn and wintering in the zone of the Western Forest-Steppe of Ukraine, by sowing high-quality basic seed, careful soil preparation and the presence of optimum environmental factors. A sufficient level of productive soil moisture, which protects young shoots from possible deficiency after germination and is a long-term source of moisture at the next stages of organogenesis, has a great influence on obtaining friendly and timely shoots. Often overwintering conditions, when plants suffer from low negative temperatures at the beginning and at the end of the winter period, ground ice crust, resumption of vegetation in winter are the causes of freezing, loss, and ultimately a decrease in yield and seed quality. It has been confirmed that an increase in the temperature regime in 244-247°C in the autumn-winter period and the optimal amount of precipitation contribute to sufficient (31.6-34.6 mm) productive soil moisture (0-20 cm), which positively influences the process of germination of soft winter wheat, provides a high percentage of field germination of seeds of varieties (93.8-94.5%), lengthens the autumn development of plants by 3-12 days, which causes 3.5-5.7% higher accumulation of sugar content in the tillering nodes and a high percentage of overwintering (up to 95.5-96.4%). Varieties of the forest-steppe ecological type of soft winter wheat have insignificant phenotypic variability of adaptive traits, therefore, in the production of grain and seed products, it is recommended to give preference to the plant varieties listed in the Register, suitable for distribution in Ukraine for the Forest-Steppe zone, Polissya. The recommendations set out in this scientific work will help agricultural producers of the studied soil and climatic zone to carry out an effective, more ecologically plastic, highly productive variety replacement

Black point of winter wheat seeds

Goal. To study infection of seeds of winter wheat varieties by «black point» and to establish the phytopathogenic composition of pathogens in the Right Bank Forest-Steppe of Ukraine. Methods. Laboratory — macroscopic analysis was performed according to DSTU 4138-2002, phytopathogenic composition was identified using nutrient medium, statistical analysis of the obtained data — calculation of the degree of pathogen severity, LSD. Results. A collection of 27 varieties of soft winter wheat was studied to determine the degree of seed infection. The highest percentage of infection was found in varieties Vodogray Bilotserkivsky (Ukraine), Adele (Russia), Balitus (Austria), Viglanka (Slovakia), Sefeg-2 (Azerbaijan). The least susceptible to the disease are varieties Daria (Croatia), Bodycek (France), Gratsia Bilotserkivska (Ukraine, Bila Tserkva DSS), Azano (Sweden), Gospodarka (Ukraine), Kozyr (Ukraine), Morozko (Russia), the number of grains with signs of darkening in the embryonic zone for these varieties did not exceed 1%. The sowing qualities of the diseased seeds were determined. The species composition of phytopathogens that cause the disease has been established. The phytopathogenic complex of seeds with the «black point» included fungi of the genera Alternaria, Fusarium, Curvularia, Bipolaris, Aspergillus, Acremoniella, Stemphillium, Sordaria and Epicoccum. Most often, the seeds were colonized by fungi of the genus Alternaria Nees. Their share in the years of research was 76.6—83.1%. Conclusions. The infection of seeds of winter wheat varieties by black point under conditions of the Right-Bank Forest-Steppe of Ukraine in 2018—2019 ranged from 0 to 19.8% depending on the variety and year of research. The phytopathogenic complex included 13 species of fungi from 9 genera: Alternaria, Fusarium, Curvularia, Bipolaris, Aspergillus, Acremoniella, Stemphillium, Sordaria, Epicoccum. The part of Alternaria spp. in the years of research was over 75%, the most common species were A. tenuissima and A. infectoria. The percentage of species from other genera was insignificant and did not exceed 4.6%.

Stabilization of soft wheat selection material which is the result of crossing with wild relatives

Aim. Testing the haploproduction ability of 30 hybrids of winter soft wheat. Methods. In vitro culture of isolated anthers of wheat. The percentage of callus and regeneration of green plants for each genotype calculated as a percentage of the planted anthers. Results. The differences in the frequency of callus induction and the ability to regenerate plants in the process of androgenesiss in vitro of winter soft wheat were detected. The microspores of 17 of 30 hybrids formed callus by in vitro anther culture were shown. The intensity of one process was different: more than half of the genotypes (18 pcs.) were characterized by a low percentage of callus (from 0.10 to 1.0%), 6 genotypes - medium (from 1.0 to 3.0%), and three - high (4.36%; 15.11% and 15.81%, respectively). Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed Samples P26 and P27 showed the highest level of callus formation. The 10 green regenerating plants were obtained. Keywords: hybrids, soft winter wheat, anther culture in vitro, callus, regeneration.

New variety of soft winter wheat Alyonushka

The article presents the main methods of creating and the results of studying a new variety of winter soft wheat Alyonushka. The variety was created at the FSBRI OP Penza Research Institute of Agriculture by the method of intraspecific hybridization of varieties with subsequent individual selection from the hybrid population F3 Kazanskaya 237 // Moshinskaya 2 / Zolushka. The characteristics of the new variety are given according to the most important economically valuable traits and properties. The new variety Alyonushka in terms of grain yield and the elements composing it, during the years of research (2015–2018), significantly exceeded the standard variety Fotinya. The average increase in grain to the standard for these years was 0.78 t / ha. The high yield of the new variety is formed due to the high mass of 1000 grains, the number and weight of grain per spike. It has high winter hardiness (88% on average), high lodging resistance (9 points) and grain quality at the level of valuable wheat. The new variety Alyonushka stably forms a completed grain (grain nature is 756-797 g / l), the content of crude protein in the grain is 14.8-16.2%, gluten is 28.2-30.2% with a gluten quality of 50-85 units. ... IDK (I-II group), baking strength of flour - 296 units, volumetric yield of bread from 100 g of flour - 780 cm3, overall assessment of bread - 4.7 points. The economic efficiency of cultivation of the Alyonushka variety in comparison with the Fotinya standard was 3780 rubles / ha. The profitability level of the new variety was 135%, which is 21% higher than that of the standard.