Molecular Genetic Evaluation of Ukrainian Flax Cultivar Homogeneity Based on Intron Length Polymorphism of Actin Genes and Microsatellite Loci

2018 ◽  
Vol 52 (6) ◽  
pp. 448-460 ◽  
Author(s):  
A. S. Postovoitova ◽  
O. Yu. Yotka ◽  
Ya. V. Pirko ◽  
Ya. B. Blume
Keyword(s):  
Length Polymorphism ◽  
Microsatellite Loci ◽  
Molecular Genetic ◽  
Genetic Evaluation ◽  
Intron Length ◽  
Intron Length Polymorphism ◽  
Actin Genes ◽  
Flax Cultivar

scholarly journals Ist and IIIrd intron length polymorphism of actin genes as a tool for flax DNA-profiling

2019 ◽  
Vol 25 ◽  
pp. 154-159
Author(s):  
A. S. Postovoitova ◽  
Ya. V. Pirko ◽  
Ya. B. Blume
Keyword(s):  
Length Polymorphism ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Intron Length ◽  
Specific Primers ◽  
Intron Length Polymorphism ◽  
Actin Genes ◽  
Linum Usitatissimum L ◽  
Species Specific ◽  
Dna Profiles

Aim. The purpose of the work was to evaluate the possibility of using the polymorphism of the Ist and IIIrd introns of actin genes for DNA plant genotyping using flax varieties as model. Methods. 16 varieties of Ukrainian flax were analyzed. PCR was conducted using self-developed species-specific primers for the Ist and IIIrd introns of flax actin genes. DNA fragments were separated by electrophoresis in a 6% polyacrylamide gel and visualized by silver stains. Results. As a result of the evaluation of the Ist and IIIrd intron length polymorphism of actin genes, the species-specific DNA profiles of 16 flax varieties containing the target amplicons were obtained. The 7 allele phenotypes (PIC = 0.62) were detected for the Ist introns of the actin genes, and 3 allelic phenotypes (PIC = 0.32) for the IIIrd intron of actin genes. The highest level of polymorphism in the flax varieties was detected by evaluating the Ist intron length polymorphism of actin genes. Conclusions. Evaluation of the polymorphism of the Ist and IIIrd introns of actin genes allows genotyping and obtaining DNA profiles of flax varieties, which demonstrates the feasibility of further using both approaches for molecular genetic analysis of plants. Keywords: gene introns, length polymorphism, actin genes, flax (Linum usitatissimum L.).

scholarly journals Estimation of genetic diversity for different species of woody plants by intron length polymorphism of beta-tubulin genes

2018 ◽  
Vol 15 (2) ◽  
pp. 159-166
Author(s):  
L. O. Kalafat ◽  
N. N. Pirko ◽  
A. Ye. Demkovych ◽  
S. N. Privalikhin ◽  
A. N. Rabokon ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Fagus Sylvatica ◽  
Length Polymorphism ◽  
Juglans Regia ◽  
Molecular Genetic ◽  
Robinia Pseudoacacia ◽  
Intron Length ◽  
Intron Length Polymorphism ◽  
Tubulin Genes ◽  
Β Tubulin

Aim. The effectiveness of using the method of intron length polymorphism of β-tubulin genes is analyzed to evaluate the genetic diversity and polymorphism of tree plants by approbating it for a broad sample of tree plants of different taxonomic positions. Method. The method of estimation intron length polymorphism of β- tubulin (tubulin base polymorphism — TBP-method) has been tested. Results. The molecular genetic profiles and the unique patterns for the Quercus robur L., Populus tremula L., Fagus sylvatica L., Fagus sylvatica f. salicifolia, Robinia pseudoacacia L., Morus alba L., Ulmus glabra Huds., Betula pendula Roth., Acer platanoides L., Acer negundo L., Acer saccharinum Marshall, Catalpa bignonioides Walter, Tilia cordata Mill., Tilia platyphyllos Scop., Aesculus hippocastanum L., Populus nigra L., Juglans regia L., Fraxinus excelsior L., Alnus glutinosa (L.) Gaertn., Ginkgo biloba L. have been created. Some common fragments inherent in individual genera within the family have been found. Conclusions. TBP-method is rather convenient and reliable. It can be used both for molecular genetic marking and for the study of intra- and interspecific polymorphism of economically valuable, horticultural and forest trees.Keywords: TBP-method, β-tubulin introns, tree plants, genetic diversity.

scholarly journals β-tubulin intron length polymorphism among forms var. glabra and var. laxa of napa cabbage

2020 ◽  
Vol 26 ◽  
pp. 87-92
Author(s):  
R. Ya. Blume ◽  
A. N. Rabokon ◽  
Ya. V. Pirko
Keyword(s):  
Length Polymorphism ◽  
Genetic Difference ◽  
Molecular Genetic ◽  
Genetic Distances ◽  
Molecular Genetic Analysis ◽  
Intron Length ◽  
Intron Length Polymorphism ◽  
Molecular Profiles ◽  
Crop Research ◽  
Β Tubulin

Aim. Main aim of this research was identification of genetic distances between different genotypes of napa cabbage (B. rapa ssp. pekinensis) and diversity identification in var. glabra and var. laxa forms. Methods. Molecular genetic analysis of napa cabbage genotypes was conducted out using method of β-tubulin intron length polymorphism (TBP). Results. Molecular profiles of different napa cabbage (B. rapa ssp. pekinensis) genotypes were identified. Number of amplified β-tubulin intron fragments was significantly varying – from 12 to 24 for each genotype. Basing on obtained results a dendrogram was built, which shows genetic distances among studied accessions. Conclusions. In present study 7 genotypes of B. rapa ssp. pekinensis were analyzed, received from IPK (Gatersleben) and Crop Research Institute (Prague) gene banks. Basing on obtained results it was established that systematic diversification of two forms var. glabra and var. laxa is not being confirmed by molecular genetic methods, such as TBP, and in this case, genetic difference between populations and cultivars was more significant. Keywords: Brassicaceae, Brassica rapa ssp. pekinensis, ILP, TBP, napa cabbage, β-tubulin intron length polymorphism.

scholarly journals γ-tubulin gene intron length polymorphism of Arabidopsis thaliana

2020 ◽  
Vol 26 ◽  
pp. 120-124
Author(s):  
A. M. Rabokon ◽  
Yu. O. Bilonozhko ◽  
A. S. PostovoitovA ◽  
L. O. Kalafat ◽  
Ya. V. Pirko ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Length Polymorphism ◽  
Molecular Genetic ◽  
Intron Length ◽  
Degenerate Primers ◽  
Tubulin Gene ◽  
Specific Primers ◽  
Intron Length Polymorphism ◽  
Genetic Studies ◽  
Dna Profile

Aims. Verification of the possibility of using the γ-tubulin gene intron length polymorphism method in genetic studies of plants on the example of Arabidopsis thaliana. Methods. The γ-tubulin gene intron length polymorphism evaluating method was used. Amplified fragments DNA were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using silver nitrate staining. Results. Arabidopsis was first time analyzed using the γ-tubulin gene intron length polymorphism method. During amplification with degenerate primers 2 amplicons (520 bp and 555 bp) were formed in all samples. However, using selected arabidopsis-specific primers for the second intron of the γ-tubulin genes, it was possible to find several samples that differ in their DNA profile. Conclusions. It is established that the proposed method can be used in molecular genetic studies of plants. Moreover, the developed specific primers for γ-tubulin gene introns can probably be used both for the study of Arabidopsis and related species. The use of degenerate primers can be useful in the study of plants for which there is no information about their genome. Keywords: molecular-genetic markers, intron length polymorphism, γ-tubulin, A. thaliana.

scholarly journals Genetic polymorphism of white mistletoe (Viscum album L.) in Ukraine

2021 ◽  
Vol 28 ◽  
pp. 36-41
Author(s):  
Yu. O. Bilonozhko ◽  
A. M. Rabokon ◽  
A. S. Postovoitova ◽  
L. O. Kalafat ◽  
N. S. Boiko ◽  
...  
Keyword(s):  
Length Polymorphism ◽  
Polyacrylamide Gel Electrophoresis ◽  
Viscum Album ◽  
Intron Length ◽  
Intron Length Polymorphism ◽  
Tubulin Genes ◽  
Silver Nitrate Staining ◽  
Dna Profiles ◽  
Different Parts ◽  
Β Tubulin

Aim. The aim of the study was to establish genetic differences between V. album growing in different parts of Ukraine. Methods. White mistletoe samples collected in different regions of Ukraine were used in the study. The method of estimating the intron length polymorphism of β-tubulin genes was used. Amplified DNA fragments were fractionated by non-denaturing polyacrylamide gel electrophoresis and visualized by silver nitrate staining. Results. The genotypes of 91 white mistletoe plants were analyzed. DNA profiles of white mistletoe with a specific amplicons of β-tubulin gene introns were obtained, which allowed to differentiate the samples from each other. Fingerprinting data were used for cluster analysis and dendrogram construction. Conclusions. It was found that the analyzed mistletoe samples did not differ by geographical factor and were characterized by a low level of genetic diversity in the studied samples. Keywords: Viscum album L., intron length polymorphism, β-tubulin, genetic variability, Ukraine.

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