scholarly journals Intron length polymorphism of actin genes as an efficient tool for the genetic profiling of selected cereals from the grass (Poaceae L.) family

2019 ◽  
Vol 2 ◽  
pp. 78-83
Author(s):  
A.S. Postovoitova ◽  
◽  
Ya.V. Pirko ◽  
Ya.B. Blume ◽  
◽  
...  
Keyword(s):  
Length Polymorphism ◽  
Intron Length ◽  
Efficient Tool ◽  
Intron Length Polymorphism ◽  
Genetic Profiling ◽  
Actin Genes

scholarly journals Ist and IIIrd intron length polymorphism of actin genes as a tool for flax DNA-profiling

2019 ◽  
Vol 25 ◽  
pp. 154-159
Author(s):  
A. S. Postovoitova ◽  
Ya. V. Pirko ◽  
Ya. B. Blume
Keyword(s):  
Length Polymorphism ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Intron Length ◽  
Specific Primers ◽  
Intron Length Polymorphism ◽  
Actin Genes ◽  
Linum Usitatissimum L ◽  
Species Specific ◽  
Dna Profiles

Aim. The purpose of the work was to evaluate the possibility of using the polymorphism of the Ist and IIIrd introns of actin genes for DNA plant genotyping using flax varieties as model. Methods. 16 varieties of Ukrainian flax were analyzed. PCR was conducted using self-developed species-specific primers for the Ist and IIIrd introns of flax actin genes. DNA fragments were separated by electrophoresis in a 6% polyacrylamide gel and visualized by silver stains. Results. As a result of the evaluation of the Ist and IIIrd intron length polymorphism of actin genes, the species-specific DNA profiles of 16 flax varieties containing the target amplicons were obtained. The 7 allele phenotypes (PIC = 0.62) were detected for the Ist introns of the actin genes, and 3 allelic phenotypes (PIC = 0.32) for the IIIrd intron of actin genes. The highest level of polymorphism in the flax varieties was detected by evaluating the Ist intron length polymorphism of actin genes. Conclusions. Evaluation of the polymorphism of the Ist and IIIrd introns of actin genes allows genotyping and obtaining DNA profiles of flax varieties, which demonstrates the feasibility of further using both approaches for molecular genetic analysis of plants. Keywords: gene introns, length polymorphism, actin genes, flax (Linum usitatissimum L.).

scholarly journals Genetic polymorphism of white mistletoe (Viscum album L.) in Ukraine

2021 ◽  
Vol 28 ◽  
pp. 36-41
Author(s):  
Yu. O. Bilonozhko ◽  
A. M. Rabokon ◽  
A. S. Postovoitova ◽  
L. O. Kalafat ◽  
N. S. Boiko ◽  
...  
Keyword(s):  
Length Polymorphism ◽  
Polyacrylamide Gel Electrophoresis ◽  
Viscum Album ◽  
Intron Length ◽  
Intron Length Polymorphism ◽  
Tubulin Genes ◽  
Silver Nitrate Staining ◽  
Dna Profiles ◽  
Different Parts ◽  
Β Tubulin

Aim. The aim of the study was to establish genetic differences between V. album growing in different parts of Ukraine. Methods. White mistletoe samples collected in different regions of Ukraine were used in the study. The method of estimating the intron length polymorphism of β-tubulin genes was used. Amplified DNA fragments were fractionated by non-denaturing polyacrylamide gel electrophoresis and visualized by silver nitrate staining. Results. The genotypes of 91 white mistletoe plants were analyzed. DNA profiles of white mistletoe with a specific amplicons of β-tubulin gene introns were obtained, which allowed to differentiate the samples from each other. Fingerprinting data were used for cluster analysis and dendrogram construction. Conclusions. It was found that the analyzed mistletoe samples did not differ by geographical factor and were characterized by a low level of genetic diversity in the studied samples. Keywords: Viscum album L., intron length polymorphism, β-tubulin, genetic variability, Ukraine.

scholarly journals Erratum to: Intron-length polymorphism identifies a Y2K4 dehydrin variant linked to superior freezing tolerance in alfalfa

2012 ◽  
Vol 124 (5) ◽  
pp. 821-824
Author(s):  
Yves Castonguay ◽  
Marie-Pier Dubé ◽  
Jean Cloutier ◽  
Réal Michaud ◽  
Annick Bertrand ◽  
...  
Keyword(s):  
Freezing Tolerance ◽  
Length Polymorphism ◽  
Intron Length ◽  
Intron Length Polymorphism

scholarly journals Intravarietal intron-length polymorphism of β-tubulin genes in belorussian landraces of Linum usitatissimum L.

2018 ◽  
Vol 22 ◽  
pp. 180-185
Author(s):  
A. M. Rabokon ◽  
Ya. B. Pirko ◽  
L. O. Kalafat ◽  
Ye. V. Guzenko ◽  
M. V. Bogdanova ◽  
...  
Keyword(s):  
Length Polymorphism ◽  
Linum Usitatissimum ◽  
Intron Length ◽  
Intron Length Polymorphism ◽  
Linum Usitatissimum L ◽  
Tubulin Genes ◽  
Molecular Phylogenetic ◽  
Silver Nitrate Staining ◽  
Molecular Selection ◽  
Β Tubulin

Aim. The aim of the work was to evaluate the possibilities of using the β-tubulin intron length (TBP, Tubulin Based Polymorphism) for genetic differentiation of ancient flax varieties (landraces), plants that was historically formed in Belarus. Methods. The β-tubulin first intron length polymorphism evaluating method (TBP) was used. Amplified fragments (β-tubulin introns) were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using silver nitrate staining. Results. The size of the amplified fragments varied from 400 bp to 1900 bp. It was found that 25 of 30 studied varieties (landraces) were genetically heterogeneous. The total number of allele phenotypes was 7, and the value of PIC (Polymorphism Information Content) varied from 0.0 to 0.72. Conclusions. The data obtained make it possible to recommend landraces as a source of genes for increasing the genetic diversity of the existing flax gene pool, and the TBP method can be applied both in molecular-phylogenetic analysis and in molecular selection of flax. Keywords: Linum usitatissimum, landrace, molecular markers, DNA fingerprinting, β-tubulin genes, intron polymorphism.

Sign in / Sign up

Export Citation Format

Share Document