The study of heterogeneity of 16S rRNA gene and groESL operone in DNA samples of Anaplasma phagocytophilum, Ehrlichia muris, and “Candidatus Neoehrlichia mikurensis” determined in Ixodes persulcatus ticks on the territory of Ural, Siberia and Far East of Russia

2011 ◽  
Vol 26 (2) ◽  
pp. 66-73 ◽  
Author(s):  
V. A. Rar ◽  
T. I. Epikhina ◽  
N. N. Livanova ◽  
V. V. Panov ◽  
E. K. Doroschenko ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Anaplasma Phagocytophilum ◽  
Far East ◽  
Rrna Gene ◽  
Ixodes Persulcatus ◽  
Far East Of Russia ◽  
Candidatus Neoehrlichia Mikurensis ◽  
Ehrlichia Muris ◽  
Neoehrlichia Mikurensis

scholarly journals Wide Distribution and Genetic Diversity of “Candidatus Neoehrlichia mikurensis” in Rodents from China

2012 ◽  
Vol 79 (3) ◽  
pp. 1024-1027 ◽  
Author(s):  
Hao Li ◽  
Jiafu Jiang ◽  
Fang Tang ◽  
Yi Sun ◽  
Zengde Li ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
16S Rrna ◽  
Phylogenetic Analyses ◽  
Wide Distribution ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Candidatus Neoehrlichia Mikurensis ◽  
Geographic Origins ◽  
Neoehrlichia Mikurensis

ABSTRACT“CandidatusNeoehrlichia mikurensis” was detected by PCR in 4.0% (34/841) of the rodents tested in this study. The 34 rodents represented nine species from seven regions of China. Phylogenetic analyses based on the partialgroELand nearly entire 16S rRNA gene sequences of the agent revealed genetic diversity, which was correlated with its geographic origins.

scholarly journals Survey of Tick-borne-disease from Ornithodoros spp. in Uninhabited Islands of Korea.

2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Sun-Woo Han ◽  
Yong-Sun Jo ◽  
Jeong Byoung Chae ◽  
Yoon Kyoung Cho ◽  
Nam-Shik Shin ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Anaplasma Phagocytophilum ◽  
Economic Losses ◽  
Rrna Gene ◽  
Gene Fragment ◽  
Sequence Identification ◽  
Tick Borne Disease ◽  
Anaplasma Bovis ◽  
Breeding Place

ObjectiveThe aim of this study is to survey data of ticks distribution of Korean islands and to investigate pathogens in Argasid ticks.IntroductionTicks and tick-borne diseases have been thought global important issues, because it's affect to animal and human health and are the cause of significant economic losses. The genus Ornithodoros spp., which is included in Family Argasidae, is usually associated with wild animals including seabirds and it was difficult to investigate because seabirds’ nests are found in inaccessible uninhabited islands. However, Ornithodoros spp. has been known for the vector of many diseases including African swine fever.MethodsIn this study, nest with soil and litter of seabirds were collected, to investigate Ornithodoros species from 9 uninhabited islands, Nan-do, Chilsan-do, Chilbal-do, Sogukhol-do, Googul-do, Gaerin-do, Sasu-do, Hong-do (Hallyeohaesang) and Dok-do located western and southern part of the Korea from July, 2017 to September in 2018. The islands are known for breeding places of migratory and resident birds. Maximum ten nests with soil and litter of seabirds were collected from one uninhabited island for the conservation of the islands environment. Ticks were collected from nest with soil and litter of seabirds using Tullgren funnel and were assayed for tick identification by PCR using 16S rRNA gene and tick-borne pathogens including Rickettia spp., Borrelia spp., Bartonella spp., Ehrlichia chaffensis, Ehrlichia canis, Anaplasma phagocytophilum and Anaplasma bovis by nested PCR.ResultsTotal 65 Ornithodoros species ticks from 338 seabird's (black-tailed gull, Larus crassirotris; streaked shearwater, Calonectris leucomelas and Swinhoe's storm petrel, Oceanodroma monorhis) nesting soil with litter in 9 uninhabited islands. In the sequence identification of 16S rRNA gene fragment of Ornithodoros species, O. capensis and O. sawaii were 37 and 28, respectively. In the analyses of tick-borne pathogens, Borrelia spp. (n=5) was detected in O. sawaii from Gaerin-do and Googul-do. The total detection rate of Borrelia sp. from Ornithodoros spp. was 7.69% (5/65).ConclusionsIn this research, we discovered that O. sawaii are habitat in west and southern part of uninhabited islands to breeding place of black-tailed gulls and streaked shearwater and O. capensis are habitat in uninhabited islands related to breeding place of streaked shearwater. This is first report of the Borrelia spp. from Ornithodoros sawaii in Korea. 

scholarly journals Anaplasma phagocytophilum in Questing Ixodes ricinus Ticks: Comparison of Prevalences and Partial 16S rRNA Gene Variants in Urban, Pasture, and Natural Habitats

2012 ◽  
Vol 79 (5) ◽  
pp. 1730-1734 ◽  
Author(s):  
Evelyn Overzier ◽  
Kurt Pfister ◽  
Claudia Thiel ◽  
Ingrid Herb ◽  
Monia Mahling ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Ixodes Ricinus ◽  
Anaplasma Phagocytophilum ◽  
Rrna Gene ◽  
Gene Variants ◽  
Habitat Types ◽  
Natural Habitats ◽  
Content Type ◽  
Ixodes Ricinus Ticks

ABSTRACTUrban, natural, and pasture areas were investigated for prevalences and 16S rRNA gene variants ofAnaplasma phagocytophilumin questingIxodes ricinusticks. The prevalences differed significantly between habitat types, and year-to-year variations in prevalence and habitat-dependent occurrence of 16S rRNA gene variants were detected.

scholarly journals PCR Amplification and Phylogenetic Analysis ofgroESL Operon Sequences from Ehrlichia ewingiiand Ehrlichia muris

2000 ◽  
Vol 38 (7) ◽  
pp. 2746-2749 ◽  
Author(s):  
John W. Sumner ◽  
Gregory A. Storch ◽  
Richard S. Buller ◽  
Allison M. Liddell ◽  
Steven L. Stockham ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Pcr Amplification ◽  
Pcr Primers ◽  
Rrna Gene ◽  
Human Pathogen ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Ehrlichia Muris

Broad-range PCR primers were used to amplify part of thegroESL operon of the canine pathogen Ehrlichia ewingii, recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris. Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comparison of 16S rRNA gene sequences. These sequences provide additional PCR targets for species for which few gene sequences have been determined.

Differentiation Of Clarias Batrachus, C. Gariepinus And Heteropneustes Fossilis By Pcr-Sequencing Of Mitochondrial 16s Rrna Gene

2015 ◽  
Vol 41 (1) ◽  
pp. 51-58
Author(s):  
Mohammad Shamimul Alam ◽  
Hawa Jahan ◽  
Rowshan Ara Begum ◽  
Reza M Shahjahan
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Fish Larva ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Valuable Insight ◽  
Multiple Sequence ◽  
Pcr Rflp ◽  
Alternative Means

Heteropneustesfossilis, Clariasbatrachus and C. gariepinus are three major catfishes ofecological and economic importance. Identification of these fish species becomes aproblem when the usual external morphological features of the fish are lost or removed,such as in canned fish. Also, newly hatched fish larva is often difficult to identify. PCRsequencingprovides accurate alternative means of identification of individuals at specieslevel. So, 16S rRNA genes of three locally collected catfishes were sequenced after PCRamplification and compared with the same gene sequences available from othergeographical regions. Multiple sequence alignment of the 16S rRNA gene fragments ofthe catfish species has revealed polymorphic sites which can be used to differentiate thesethree species from one another and will provide valuable insight in choosing appropriaterestriction enzymes for PCR-RFLP based identification in future. Asiat. Soc. Bangladesh, Sci. 41(1): 51-58, June 2015

Marine mammals are natural hosts of Oceanivirga salmonicida, a bacterial pathogen of Atlantic salmon

2020 ◽  
Vol 139 ◽  
pp. 161-174
Author(s):  
R Palmer ◽  
GTA Fleming ◽  
S Glaeser ◽  
T Semmler ◽  
A Flamm ◽  
...  
Keyword(s):  
16S Rrna ◽  
Atlantic Salmon ◽  
16S Rrna Gene ◽  
Marine Mammals ◽  
Rrna Gene ◽  
Bacterial Disease ◽  
Common Dolphin ◽  
Stenella Coeruleoalba ◽  
Striped Dolphin ◽  
Natural Hosts

During 1992 and 1993, a bacterial disease occurred in a seawater Atlantic salmon Salmo salar farm, causing serious mortalities. The causative agent was subsequently named as Oceanivirga salmonicida, a member of the Leptotrichiaceae. Searches of 16S rRNA gene sequence databases have shown sequence similarities between O. salmonicida and uncultured bacterial clones from the digestive tracts of marine mammals. In the current study, oral samples were taken from stranded dolphins (common dolphin Delphinus delphis, striped dolphin Stenella coeruleoalba) and healthy harbour seals Phoca vitulina. A bacterium with growth characteristics consistent with O. salmonicida was isolated from a common dolphin. The isolate was confirmed as O. salmonicida, by comparisons to the type strain, using 16S rRNA gene, gyrB, groEL, and recA sequence analyses, average nucleotide identity analysis, and MALDI-TOF mass spectrometry. Metagenomic analysis indicated that the genus Oceanivirga represented a significant component of the oral bacterial microbiomes of the dolphins and seals. However, sequences consistent with O. salmonicida were only found in the dolphin samples. Analyses of marine mammal microbiome studies in the NCBI databases showed sequences consistent with O. salmonicida from the common dolphin, striped dolphin, bottlenose dolphin Tursiops truncatus, humpback whale Megaptera novaeangliae, and harbour seal. Sequences from marine environmental studies in the NCBI databases showed no sequences consistent with O. salmonicida. The findings suggest that several species of marine mammals are natural hosts of O. salmonicida.

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