Determination of capability of biofilm formation of strains s.aureus selected from the upper respiratory tract

2021 ◽  
Vol 43 (3-4) ◽  
pp. 42-45
Author(s):  
N. Y. Kravets ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Respiratory Tract ◽  
Biofilm Formation ◽  
Control Measures ◽  
Past Century ◽  
Upper Respiratory Tract ◽  
Chronic Infections ◽  
Inflammatory Lesions ◽  
Acute Infections ◽  
Upper Respiratory

Millions of people have died from acute infections in the past century, but they have been effectively fought through the development of modern vaccines, antibiotics and infection control measures. Chronic infections are slower than acute infections, and the symptoms are often vague, difficult, and sometimes impossible to cure with antibiotics. Important signs of chronic biofilm infections are extreme resistance to antibiotics and many other common antimicrobials, as well as the extraordinary ability to avoid the host’s defenses. One such disease is chronic inflammatory lesions of the tonsils, the main infectious agents of which are gram-positive cocci, strains Staphylococcus spp., Streptococcus spp. The purpose of the study of the ability of strains of Staphylococcus aureus to form a biofilm isolated from the surface of the epithelium of the upper respiratory tract of children. Clinical strains of Staphylococcus aureus bacteria obtained from the oropharynx of 32 children with tonsils affected by the inflammatory process at the age of 4-12 years (median – 7) were studied. The results of microbiological examination of biomaterial obtained from children with chronic inflammatory lesions of the tonsils showed that in 32 samples 25 strains of S. aureus were identified, 12 of them (48%) are capable of forming a biofilm, and 13 strains (52%) (not adhesive) are not had this ability. The study of the dynamics of biofilm formation by selected strains of S. aureus showed an increase in optical density (OS) during three days of cultivation, ranging from 0.143

scholarly journals Asymptomatic COVID-19 Adult Outpatients identified as Significant Viable SARS-CoV-2 Shedders 

2021 ◽  
Author(s):  
Marie Glenet ◽  
Anne-Laure Lebreil ◽  
Laetitia Heng ◽  
Yohan N’Guyen ◽  
Ittah Meyer ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Control Measures ◽  
Upper Respiratory Tract ◽  
Rna Detection ◽  
Viral Loads ◽  
Infection Control Measures ◽  
Infected Adult ◽  
Asymptomatic Adult ◽  
Upper Respiratory ◽  
Kinetics Of

Abstract Differential kinetics of RNA loads and infectious viral levels in the upper respiratory tract between asymptomatic and symptomatic SARS-CoV-2 infected adult outpatients remain unclear limiting recommendations that may guide clinical management, infection control measures and occupational health decisions. In the present investigation, 496 (2.5%) of 17,911 French adult outpatients were positive for an upper respiratory tract SARS-CoV-2 RNA detection by a quantitative RT-PCR assay, of which 180 (36.3%) were COVID-19 asymptomatic. Of these adult asymptomatic viral shedders, 84.4% had mean to high RNA viral loads (Ct values<30) which median value was significantly higher than that observed in symptomatic subjects (P=0.029), and 50.6% were positive by cell culture assays of their upper respiratory tract specimens. Our findings indicate that COVID-19 asymptomatic adult outpatients are significant viable SARS-CoV-2 shedders in their upper respiratory tract playing a major potential role as SARS-CoV-2 transmitters in various epidemiological transmission chains, promoting COVID-19 resurgence in populations.

A Potential Benefit of Hypochlorous Acid - Facial Sanitisation: A Review

2021 ◽  
Author(s):  
Avis Aman Nowbuth ◽  
Josh Barrie Armstrong ◽  
Thomas Eugene Cloete ◽  
Pieter Rousseau Fourie
Keyword(s):  
Respiratory Tract ◽  
Biofilm Formation ◽  
Respiratory Tract Infections ◽  
Microbial Contamination ◽  
Hypochlorous Acid ◽  
Skin Infections ◽  
Upper Respiratory Tract ◽  
The Face ◽  
Upper Respiratory ◽  
Tract Infections

Sanitisation has become a major component of everyday life, with emphasis on the hands and surfaces. The face remains unsanitised due to the lack of an acceptable sanitiser. The use of masks has been mandated to reduce the spread of the pathogens by covering the face, however, there remain issues with the use of personal protective equipment. The face remains a harbour for upper respiratory tract infections, with constant deposition of microbes. By reducing microbial load, the risk of both infection and severity are reduced. HOCl has proven antimicrobial and anti-inflammatory activity, including efficacy against SARS-CoV-2. A facial sanitiser, alongside hand sanitisers and masks, improves protection against SARS-CoV-2. The advantages of regular sanitising of the face and mask include reduced level of microbial contamination, risk of biofilm formation, and respiratory tract and skin infections. HOCl was reviewed as a face and mask sanitiser, concluding that it was an ideal product.

scholarly journals The Haemophilic Bacteria of the Upper Respiratory Tract, the Appearance of Virulent forms in Relation to Upper Respiratory Infections

1934 ◽  
Vol 34 (2) ◽  
pp. 195-202 ◽  
Author(s):  
L. Hoyle
Keyword(s):  
Respiratory Tract ◽  
Respiratory Infections ◽  
Bacterial Flora ◽  
The Other ◽  
Upper Respiratory Tract ◽  
Upper Respiratory Infections ◽  
Acute Infections ◽  
Other Hand ◽  
Upper Respiratory ◽  
Normal Health

In previous studies of the bacterial flora of the upper respiratory tract and its variations during attacks of acute coryza (Hoyle, 1932), it was found that there was in many cases a striking relationship between the appearance of certain organisms with definite pathogenic properties towards the lower animals, in the upper respiratory tract, and the occurrence of acute infections. This relationship was especially marked in the case of the influenza bacillus, and it was suggested that this organism played a prominent part in the aetiology of upper respiratory infections. The significance of the influenza bacillus in acute coryza has been noted by various workers (Noble, Fisher and Brainard, 1928; Burky and Smillie, 1929; Webster and Clow, 1932; Kneeland and Dawes, 1932), but on the other hand various observers have found the incidence of influenza bacilli in the respiratory tract to be the same in normal health and in acute coryza, and Fleming (1929) and Fleming and Maclean (1930) have devised a technique by means of which they claim to have isolated influenza bacilli in 100 per cent, of normal throats. One possible explanation of these conflicting results may be found in the absence of any very exact criterion of what constitutes a typical influenza bacillus.

Place of phytotherapy in the treatment of acute infections of upper respiratory tract and upper gastrointestinal tract

2018 ◽  
Vol 72 (4) ◽  
pp. 42-50 ◽  
Author(s):  
Wioletta Pietruszewska ◽  
Magda Barańska ◽  
Jakub Wielgat
Keyword(s):  
Gastrointestinal Tract ◽  
Respiratory Tract ◽  
Upper Gastrointestinal Tract ◽  
Upper Respiratory Tract ◽  
Persistent Symptoms ◽  
Acute Infections ◽  
Intranasal Steroids ◽  
Upper Respiratory ◽  
Tract Infections ◽  
Upper Gastrointestinal

Acute infections of the upper respiratory tract and upper gastrointestinal tract (mouth and throat) are still the most common reason for reporting to the general practitioner or ENT specialist. Despite the fact that the most common causative agent of these diseases are virurs, antibiotics are still flavored in about 60-80% of patients who report to the doctor. In consensus such as EPOS, WHO reports and other local / national reports, there is a need to limit antibacterial treatment for symptomatic and anti-inflammatory drugs. In addition to intranasal steroids and mucocutaneous decongestants, supplementary treatment should be considered which resolves persistent symptoms. Plant preparations are used here. The growing role of rational phytotherapy is emphasized, especially due to the most frequent etiological factor of these infections, i.e. viruses. Modern phytotherapy is part of pharmacology, and all preparations based on plants meet the standards for medicines and are subject to standard registration procedures. The article discusses the use and mechanism of action of selected herbs, among others: sorrel, elderberry, yarrow and horsetail in the treatment of upper respiratory tract infections and upper gastrointestinal tract. The phytotherapy show good efficacy and tolerance, and can be used both in children and adults in recurrent infections of the upper respiratory tract. It is worth paying attention to the combined preparations, the components of which have the effect confirmed by randomized trials and long-term registration for use in treatment, both during the first signs of cold and its further duration, and supportive in chronic inflammation especially of the paranasal sinuses.

scholarly journals A Potential Benefit of Hypochlorous Acid - Facial Sanitisation

2021 ◽  
Author(s):  
Avis Aman Nowbuth ◽  
Josh Barrie Armstrong ◽  
Thomas Eugene Cloete ◽  
Pieter Rousseau Fourie
Keyword(s):  
Respiratory Tract ◽  
Biofilm Formation ◽  
Respiratory Tract Infections ◽  
Microbial Contamination ◽  
Hypochlorous Acid ◽  
Skin Infections ◽  
Upper Respiratory Tract ◽  
The Face ◽  
Upper Respiratory ◽  
Tract Infections

Sanitisation has become a major component of everyday life, with emphasis on the hands and surfaces. The face remains unsanitised due to the lack of an acceptable sanitiser. The use of masks has been mandated to reduce the spread of the pathogens by covering the face, however, there remain issues with the use of personal protective equipment. The face remains a harbour for upper respiratory tract infections, with constant deposition of microbes. By reducing microbial load, the risk of both infection and severity are reduced. HOCl has proven antimicrobial and anti-inflammatory activity, including efficacy against SARS-CoV-2. A facial sanitiser, alongside hand sanitisers and masks, improves protection against SARS-CoV-2. The advantages of regular sanitising of the face and mask include reduced level of microbial contamination, risk of biofilm formation, and respiratory tract and skin infections. HOCl was reviewed as a face and mask sanitiser, concluding that it was an ideal product.

scholarly journals INFLUENCE OF LACTOBACILLUS SPP. ON COLONIZATION AND ANTI-INFECTIOUS RESISTANCE OF THE MUCOUS MEMBRANES OF THE UPPER RESPIRATORY TRACT

2017 ◽  
Author(s):  
S. V. Kalinichenko ◽  
O. O. Korotkykh ◽  
S. I. Pokhil ◽  
M. G. Bakumenko
Keyword(s):  
Staphylococcus Aureus ◽  
Respiratory Tract ◽  
Immunoglobulin A ◽  
Secretory Immunoglobulin A ◽  
Upper Respiratory Tract ◽  
Colonization Resistance ◽  
Mucous Membranes ◽  
Upper Respiratory ◽  
Lactobacillus Spp ◽  
Secretory Immunoglobulin

Background. Lactobacilli are very important for the formation of colonization resistance and have pronounced antagonistic effect against a wide range of microorganisms. That is why the lactobacilli have extensive use as a component of classic probiotic agents that are widely used to prevent and treat dysbiotic conditions of digestive and genital systems of people.Objective. The aim of the research was to study the effect of lactobacilli on anti-infectious resistance of mucous membranes of upper respiratory tract.Methods. The colonization degree (lg CFU / g) of nasal mucosal membranes by Lactobacillus spp. and S. aureus was determined in all carriers before the experiment. Also, the level of lysozyme and secretory immunoglobulin A (sIgA) in nasal secretions cavities was identified.Results. It was established a clear dysfunction of anti-infectious resistance in carriers of Staphylococcus aureus - a decrease of colonization resistance and local immunity of mucous membranes of upper respiratory tract. As for the anti-infectious resistance of nasal mucosal of S. aureus carriers, the level of lysozyme and secretory immunoglobulin A gradually increased after the application of probiotic strain L. rhamnosus GG, and in 21 days it reached rates of healthy individuals.Conclusions. It was found out that probiotics for nasal passages sanitation in Staphylococcus aureus carriers lead to gradual eradication of the pathogen (S. aureus) with restoration of colonization and anti-infectious resistance, mucous membranes and upper respiratory tract.

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