scholarly journals Experimental model of chronic focal osteomyelitis for in vivo studies

2021 ◽  
Vol 10 (1) ◽  
pp. 71-77
Author(s):  
V.N. Mitrofanov ◽  
◽  
O.P. Zhivtsov ◽  
N.Yu. Orlinskaya ◽  
D.V. Davydenko ◽  
...  
Keyword(s):  
Experimental Model ◽  
Chronic Osteomyelitis ◽  
Dynamic Assessment ◽  
Infectious Agent ◽  
Bone Defects ◽  
Surgical Debridement ◽  
In Vivo Studies ◽  
Material Research ◽  
Experimental Animals

Introduction. The article describes an experimental model of chronic suppurative osteomyelitis in a rabbit. As new therapeutic and diagnostic algorithms for the supervision of patients with osteomyelitis appear, there is an growing need to compare the methods of surgical debridement and plasty of bone defects in an infectious process, in order to create experimental standardized pathological conditions as close to the clinical course of the disease in humans as possible. The aim of the study was to develop an experimental model of a standardized chronic purulent bone cavity, suitable for a comprehensive assessment of surgical debridement effectiveness and osteoplastic properties of bone substitute materials. Materials and methods. A standardized defect of the tibia in 24 rabbits was formed. The Staphylococcusaureus strain was used as an infectious agent. A dynamic assessment of the main indicators of blood counts in animals was carried out. The formation of chronic osteomyelitis was evaluated using radiography, com-puted tomography methods and histological studies. Results. It was shown that purulent bone wound developed in experimental animals with the technique cre-ated, and a defect with signs of a chronic purulent-inflammatory process was demonstrated. Conclusion. The proposed model of chronic osteomyelitis is reproducible. Operational flexibility and identi-cal in size and location bone defects allow to use this model in new osteoplastic material research. Keywords: chronic osteomyelitis, experiment, experimental animals

Evaluation of the Anti-Tumor Activity of Niclosamide Nanoliposomes Against Colon Carcinoma

2020 ◽  
Vol 13 (3) ◽  
pp. 245-250
Author(s):  
Mahdi Hatamipour ◽  
Mahmoud R. Jaafari ◽  
Amir A. Momtazi-Borojeni ◽  
Mahin Ramezani ◽  
Amirhossein Sahebkar
Keyword(s):  
Tumor Growth ◽  
Experimental Model ◽  
Colon Carcinoma ◽  
Carcinoma Cells ◽  
In Vivo Studies ◽  
Colon Carcinoma Cells ◽  
Ct26 Colon Carcinoma ◽  
Anti Tumor Activity

Background and Aims: Niclosamide is an established anti-helminthic drug, which has recently been shown to inhibit the growth of various cancer cells. To exploit the potential anti-tumor activity of this drug for systemic use, the problem of low aqueous solubility should be addressed. The present study tested the in vivo anti-tumor effects of a recently developed nanoliposomal preparation of niclosamide in an experimental model of colon carcinoma. Methods : The cytotoxicity of nanoliposomal niclosamide on CT26 colon carcinoma cells was evaluated using the MTT test. Inhibition of tumor growth was investigated in BALB/c mice bearing CT26 colon carcinoma cells. The animals were randomly divided into 4 groups including: 1) untreated control, 2) liposomal doxorubicin (15 mg/kg; single intravenous dose), 3) liposomal niclosamide (1 mg/kg/twice a week; intravenously for 4 weeks), and 4) free niclosamide (1 mg/kg/twice a week; intravenously for 4 weeks). To study therapeutic efficacy, tumor size and survival were monitored in 2-day intervals for 40 days. Results: In vitro results indicated that nanoliposomal and free niclosamide could exert cytotoxic effects with IC50 values of 4.5 and 2.5 μM, respectively. According to in vivo studies, nanoliposomal niclosamide showed a higher growth inhibitory activity against CT26 colon carcinoma cells compared with free niclosamide as revealed by delayed tumor growth and prolongation of survival. Conclusion : Nnaoliposomal encapsulation enhanced anti-tumor properties of niclosamide in an experimental model of colon carcinoma.

Possible role for chondroitin sulfate in urolithiasis: In vivo studies in an experimental model

1992 ◽  
Vol 208 (1-2) ◽  
pp. 1-8 ◽  
Author(s):  
Y.M. Michelacci ◽  
M.A. Boim ◽  
C.T. Bergamaschi ◽  
R.M. Rovigatti ◽  
N. Schor
Keyword(s):  
Chondroitin Sulfate ◽  
Experimental Model ◽  
In Vivo Studies

scholarly journals Gentamicin-loaded silk/nanosilver composite scaffolds for MRSA-induced chronic osteomyelitis

2019 ◽  
Vol 6 (5) ◽  
pp. 182102 ◽  
Author(s):  
Peng Zhang ◽  
Jianzhong Qin ◽  
Bo Zhang ◽  
Yi Zheng ◽  
Lingyan Yang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Formic Acid ◽  
Chronic Osteomyelitis ◽  
Antibacterial Properties ◽  
Bone Defects ◽  
Composite Scaffolds ◽  
Effective Inhibition

Methicillin-resistant Staphylococcus aureus (MRSA) often induces chronic osteomyelitis and then bone defects. Here, gentamicin-loaded silk/nanosilver composite scaffolds were developed to treat MRSA-induced chronic osteomyelitis. AgNO 3 was reduced with silk as a reducing agent in formic acid, forming silver nanoparticles in situ that were distributed uniformly in the composite scaffolds. Superior antibacterial properties against MRSA were achieved for the composite scaffolds, without the compromise of osteogenesis capacity. Then gentamicin was loaded on the scaffolds for better treatment of osteomyelitis. In vivo results showed effective inhibition of the growth of MRSA bacteria, confirming the promising future in the treatment of chronic osteomyelitis.

scholarly journals In VivoOsteogenesis of Vancomycin Loaded Nanohydroxyapatite/Collagen/Calcium Sulfate Composite for Treating Infectious Bone Defect Induced by Chronic Osteomyelitis

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Xiaojie Lian ◽  
Kezheng Mao ◽  
Xi Liu ◽  
Xiumei Wang ◽  
Fuzhai Cui
Keyword(s):  
Bone Graft ◽  
Bone Defect ◽  
Calcium Sulfate ◽  
Chronic Osteomyelitis ◽  
Rabbit Model ◽  
Calcium Sulphate ◽  
Bone Defects ◽  
Bone Graft Substitute ◽  
Calcium Sulphate Hemihydrate

A novel antibacterial bone graft substitute was developed to repair bone defects and to inhibit related infections simultaneously. This bone composite was prepared by introducing vancomycin (VCM) to nanohydroxyapatite/collagen/calcium sulphate hemihydrate (nHAC/CSH). XRD, SEM, and CCK-8 tests were used to characterize the structure and morphology and to investigate the adhesion and proliferation of murine osteoblastic MC3T3-E1 cell on VCM/nHAC/CSH composite. The effectiveness in restoring infectious bone defects was evaluatedin vivousing a rabbit model of chronic osteomyelitis. Ourin vivoresults implied that the VCM/nHAC/CSH composite performed well both in antibacterial ability and in bone regeneration. This novel bone graft substitute should be very promising for the treatment of bone defect-related infection in orthopedic surgeries.

scholarly journals Pig as an experimental model for in vitro and in vivo studies of radiosensitivity of B lymphocytes and NK cell

2008 ◽  
Vol 43 (5) ◽  
Author(s):  
Z. Sinkorova ◽  
L. Zarybnicka ◽  
Z. Vilasova ◽  
J. Sinkora
Keyword(s):  
Experimental Model ◽  
B Lymphocytes ◽  
Nk Cell ◽  
In Vivo Studies

scholarly journals Systems pharmacology based approach to investigate the in-vivo therapeutic efficacy of Albizia lebbeck (L.) in experimental model of Parkinson’s disease

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Uzma Saleem ◽  
Zohaib Raza ◽  
Fareeha Anwar ◽  
Zunera Chaudary ◽  
Bashir Ahmad
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Experimental Model ◽  
Mechanism Of Action ◽  
System Level ◽  
In Vivo Studies ◽  
Substantia Nigra Pars Compacta ◽  
Systems Pharmacology ◽  
Albizia Lebbeck

Abstract Background Parkinson’s disease (PD) is a progressive neurodegenerative disorder characterized by loss of dopaminergic neurons in substantia nigra pars compacta and clinically manifested mainly with motor dysfunctions. Plants are rich source of medicinally important bioactive compounds and inhabitants of underdeveloped countries used plants for treatment of various ailments. Albizia lebbeck has been reported to possess antioxidant and neuroprotective properties that suggest the evaluation of its traditional therapeutic potential in neurodegenerative diseases. The aim of present study was to validate the traditional use of Albizia lebbeck (L.) and delineate its mechanism of action in PD. The systems pharmacology approach was employed to explain the Albizia lebbeck (L.) mechanism of action in PD. Methods The haloperidol-induced catalepsy was adopted as experimental model of PD for in-vivo studies in wistar albino rats. The systems pharmacology approach was employed to explain the Albizia lebbeck (L.) mechanism of action in PD. Results In-vivo studies revealed that Albizia lebbeck improved the motor functions and endurance as demonstrated in behavioral studies which were further supported by the rescue of endogenous antioxidant defense and reversal of ultrastructural damages in histological studies. System pharmacology approach identified 25 drug like compounds interacting with 132 targets in a bipartite graph that revealed the synergistic mechanism of action at system level. Kaemferol, phytosterol and okanin were found to be the important compounds nodes with prominent target nodes of TDP1 and MAPT. Conclusion The therapeutic efficiency of Albizia lebbeck in PD was effectively delineated in our experimental and systems pharmacology approach. Moreover, this approach further facilitates the drug discovery from Albizia lebbeck for PD.

In vitro gene expression and preliminary in vivo studies of temperature-dependent titania–graphene nanocomposites for bone replacement applications

RSC Advances ◽  
2014 ◽  
Vol 4 (83) ◽  
pp. 43951-43961 ◽  
Author(s):  
K. Kavitha ◽  
W. Chunyan ◽  
D. Navaneethan ◽  
V. Rajendran ◽  
Suresh Valiyaveettil ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Defects ◽  
In Vivo Studies ◽  
Temperature Dependent ◽  
Graphene Nanocomposites ◽  
Bone Replacement ◽  
Potential Applications

To meet the demand for biomaterials due to increasing bone defects and damage, we sought to synthesize titania–graphene nanocomposites at different sintering temperatures and then optimize them to explore their potential applications in biomaterials.

scholarly journals Reconstructing Bone with Natural Bone Graft: A Review of In Vivo Studies in Bone Defect Animal Model

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 999 ◽  
Author(s):  
Mengying Liu ◽  
Yonggang Lv
Keyword(s):  
Bone Graft ◽  
Bone Defect ◽  
Bone Structure ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Bone Defects ◽  
Autologous Bone Graft ◽  
In Vivo Studies ◽  
Natural Bone

Bone defects caused by fracture, disease or congenital defect remains a medically important problem to be solved. Bone tissue engineering (BTE) is a promising approach by providing scaffolds to guide and support the treatment of bone defects. However, the autologous bone graft has many defects such as limited sources and long surgical procedures. Therefore, xenograft bone graft is considered as one of the best substitutions and has been effectively used in clinical practice. Due to better preserved natural bone structure, suitable mechanical properties, low immunogenicity, good osteoinductivity and osteoconductivity in natural bone graft, decellularized and demineralized bone matrix (DBM) scaffolds were selected and discussed in the present review. In vivo animal models provide a complex physiological environment for understanding and evaluating material properties and provide important reference data for clinical trials. The purpose of this review is to outline the in vivo bone regeneration and remodeling capabilities of decellularized and DBM scaffolds in bone defect models to better evaluate the potential of these two types of scaffolds in BTE. Taking into account the limitations of the state-of-the-art technology, the results of the animal bone defect model also provide important information for future design of natural bone composite scaffolds.

scholarly journals Analysis of the behavior of a biomaterial based on wollastonite/TCP in the implant process of an experimental model of critical bone defects

2021 ◽  
Vol 10 (7) ◽  
pp. e55110716800
Author(s):  
Mauricio Mitsuo Monção ◽  
Raísa Cavalcante Dourado ◽  
Luísa Queiroz Vasconcelos ◽  
Isabela Cerqueira Barreto ◽  
Roberto Paulo Correia de Araújo
Keyword(s):  
Experimental Model ◽  
Bone Defect ◽  
Blood Clot ◽  
Study Groups ◽  
Bone Defects ◽  
Bone Tissue Regeneration ◽  
Control Group ◽  
Hydrophilic Behavior ◽  
Critical Bone Defects

This study analyzes the clinical, macroscopic and radiographic characteristics of a biomaterial with different proportions of wolastonite (W) and tricalcium phosphate (TCP) on bone tissue regeneration during the implantation process of an experimental model of critical bone defects. Fifteen Wistar rats were used, randomly distributed in 5 groups (n = 3), with a bone defect created on an 8.0 mm diameter calvaria. 4 groups received implants with different proportions of W%/TCP%, referred to as W20/TCP80, W40/TCP60, W60/TCP40 and W80/TCP20. The fifth control group (GC) was filled with blood clot only. Clinical evaluation was performed every 24 hours, and after 7 days, the animals were euthanized. The calvaria were dissected and analyzed macroscopically and by radiography. All study groups showed a satisfactory clinical evolution. The macroscopic analysis showed filling of the bone defect with granules surrounded by newly formed tissue, and the radiographic analysis showed different patterns of displacement of the biomaterial. The study concluded that the different proportions of W%/TCP% were well tolerated by the study groups and demonstrated biocompatibility. The enhanced hydrophilic behavior of the W40/TCP60, W60/TCP40 and W80/TCP20 groups favored the application in the experimental model in vivo.

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