Immunohistochemical Study of Intermediate Filament Proteins on Routinely Processed, Celloidin-embedded Human Temporal Bone Sections by Using a New Technique for Antigen Retrieval

1993 ◽  
Vol 113 (1) ◽  
pp. 48-54 ◽  
Author(s):  
Shan-Rong Shi ◽  
Atul K. Tandon ◽  
Rebecca R. M. Haussmann ◽  
Krishan L. Kalra ◽  
Clive R. Taylor
Keyword(s):  
Temporal Bone ◽  
Intermediate Filament ◽  
Immunohistochemical Study ◽  
New Technique ◽  
Antigen Retrieval ◽  
Intermediate Filament Proteins ◽  
Human Temporal Bone ◽  
A New Technique

A new technique for the study of temporal bone pathology

1983 ◽  
Vol 8 (2) ◽  
pp. 77-85 ◽  
Author(s):  
L. MICHAELS ◽  
MEHER WELLS ◽  
A. FROHLICH
Keyword(s):  
Temporal Bone ◽  
New Technique ◽  
Bone Pathology ◽  
A New Technique

Immunohistochemistry of Lymphocytes and Macrophages in Human Celloidin-Embedded Temporal Bone Sections with Acute Otitis Media

1997 ◽  
Vol 106 (8) ◽  
pp. 662-668 ◽  
Author(s):  
Tetsuya Ganbo ◽  
Isamu Sando ◽  
Chiaki Suzuki ◽  
Carey D. Balaban ◽  
Masaharu Sudo
Keyword(s):  
Temporal Bone ◽  
Otitis Media ◽  
Acute Otitis Media ◽  
Microwave Treatment ◽  
Antigen Retrieval ◽  
Suppurative Otitis Media ◽  
Human Temporal Bone ◽  
Air Cells ◽  
Immunohistochemical Analyses ◽  
Mastoid Air Cells

Immunohistochemical analyses were used to investigate the distribution of lymphocytes and macrophages in routine human temporal bone sections obtained from a subject with acute suppurative otitis media. Primary antibodies specific for human CD3 and CD43 (T-lymphocytes), CD20 (B-lymphocytes), CD45 (leukocyte common antigen), and CD68 (macrophages) were used. As a pretreatment, the sections were soaked in antigen retrieval solution (saturated sodium hydroxide-methanol solution in methanol at a ratio of 1:3). A second antigen retrieval procedure (microwave treatment in 1 % zinc sulfate) was also employed for identifying CD3-positive cells. Then the avidin-biotin-peroxidase complex technique was performed. Positive reactions to all antibodies but anti-CD68 were observed in the mucosa of the eustachian tube, tympanic cavity, and mastoid air cells. Particularly, cells positive to anti-CD3 or anti-CD43 were making a diffuse invasion upon the lamina propria. CD68-positive cells were scattered only in the effusion of mastoid air cells. These results suggest that the retrospective immunohistochemical study of archival temporal bone sections is a promising approach to investigate the pathogenesis of otitis media.

scholarly journals A technique for retrieving antigens in formalin-fixed, routinely acid-decalcified, celloidin-embedded human temporal bone sections for immunohistochemistry.

1992 ◽  
Vol 40 (6) ◽  
pp. 787-792 ◽  
Author(s):  
S R Shi ◽  
C Coté ◽  
K L Kalra ◽  
C R Taylor ◽  
A K Tandon
Keyword(s):  
Temporal Bone ◽  
Room Temperature ◽  
Antigen Retrieval ◽  
Positive Staining ◽  
Retrieval Technique ◽  
Published Report ◽  
Human Temporal Bone ◽  
Antigen Loss ◽  
Formalin Fixed ◽  
Fixed Acid

The application of immunohistochemistry to routinely decalcified, celloidin-embedded human temporal bone sections has been hampered because of antigen loss during processing of the specimens. To our knowledge, there has been no published report to date describing immunohistochemical staining of such tissues suitable for examination by light microscopy. Here we report a novel antigen retrieval technique which can be successfully used to stain a variety of antigens in routinely formalin-fixed, trichloroacetic acid-decalcified, celloidin-embedded human temporal bone sections. The new procedure reported here for decalcified human temporal bone tissues simply requires immersing slides for 30 min at room temperature in an antigen retrieval solution. A total of 60 decalcified, celloidin-embedded human temporal bone tissues were tested with monoclonal antibodies (MAb) to 15 different antigens. Of these, 12 MAb showed definite positive staining, while three were negative. This technique may prove very useful in studying the expression of various antigens by immunohistochemistry in formalin-fixed, acid-decalcified, celloidin-embedded tissues.

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