Some Numerical Methods of Estimating Acid-Base Variables in Normal Human Blood with a Haemoglobin Concentration of 5g/100 cm3

1973 ◽  
Vol 32 (4) ◽  
pp. 289-295 ◽  
Author(s):  
S. Englesson ◽  
S. Grevsten ◽  
Å. Olin
1961 ◽  
Vol 39 (10) ◽  
pp. 1489-1491 ◽  
Author(s):  
N. Chandrasekhar

A study of 200 normal human blood samples, by the agar electrophoresis method, revealed that human haemolyzates contain two minor proteins in addition to the normal adult haemoglobin. The two minor proteins appearing in very low concentrations are non-haemoglobin in character, give a negative reaction to benzidine test, but a positive reaction to amido-schwarz. These two proteins have an electrophoretic mobility similar to that of γ-globulin of human serum, but appear as sharp distinct bands on the agar plate. The concentration of these non-haemoglobin components bear no correlation to the blood groups of the samples or to the haemoglobin concentration itself, but the components have some electrophoretic characteristics similar to some of the abnormal haemoglobins.


1977 ◽  
Vol 252 (7) ◽  
pp. 2331-2337 ◽  
Author(s):  
R M Winslow ◽  
M L Swenberg ◽  
R L Berger ◽  
R I Shrager ◽  
M Luzzana ◽  
...  

1956 ◽  
Vol 218 (1) ◽  
pp. 255-259
Author(s):  
John D. Evans ◽  
Jerome M. Waldron ◽  
Nadia L. Oleksyshyn ◽  
Roy W. Riemenschneider

1969 ◽  
Vol 26 (1) ◽  
pp. 1-3 ◽  
Author(s):  
E W Merrill ◽  
C S Cheng ◽  
G A Pelletier

Nature ◽  
1967 ◽  
Vol 215 (5109) ◽  
pp. 1483-1485 ◽  
Author(s):  
SOSAMMA JOHN ◽  
NATHAN BERGER ◽  
MARY JO BONNER ◽  
JULIUS SCHULTZ

1979 ◽  
Vol 46 (2) ◽  
pp. 381-386 ◽  
Author(s):  
N. Takano ◽  
M. J. Lever ◽  
C. J. Lambertsen

An acid-base nomogram for chimpanzee blood was constructed. Blood was drawn from eight lightly anesthetized chimpanzees. Each sample of blood was oxygenated and nine aliquots were prepared with three different concentrations of hemoglobin and three different amounts of added acid or base. Each aliquot was equilibrated at two PCO2 levels and the pH was measured and plotted on pH-logPCO2 coordinates. Using the intersection point of these pH-logPCO2 lines as a point of equal hemoglobin-independent “base excess” for each condition, values for true base excess were plotted. Connecting these values provided a Cartesian PCO2-pH base excess nomogram for the chimpanzee comparable to that devised by Siggaard-Andersen for humans. Examination of blood from normal human subjects by the same methods showed no appreciable differences from the original Siggaard-Andersen nomogram. However, the PCO2-pH-base excess nomogram for chimpanzee blood deviated slightly from that for human blood. It is possible that the deviation is related to an arterial bicarbonate concentration in the chimpanzee slightly higher than that in human.


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