Analysis of interleukin-10 promoter single nucleotide polymorphisms and risk of non-Hodgkin lymphoma in a Malaysian population

2014 ◽  
Vol 56 (1) ◽  
pp. 163-168 ◽  
Author(s):  
Yat-Yuen Lim ◽  
Yoon-Ming Chin ◽  
Mei-Chee Tai ◽  
Somayeh Fani ◽  
Kian-Meng Chang ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Hodgkin Lymphoma ◽  
Interleukin 10 ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Malaysian Population ◽  
Non Hodgkin Lymphoma

Epstein-Barr Virus Infection and Single Nucleotide Polymorphisms in the Promoter Region of Interleukin 10 Gene in Patients With Hodgkin Lymphoma

2007 ◽  
Vol 131 (11) ◽  
pp. 1691-1696 ◽  
Author(s):  
Glenda Nicioli da Silva ◽  
Maura Moscardi Bacchi ◽  
Cláudia Aparecida Rainho ◽  
Deilson Elgui de Oliveira
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Hodgkin Lymphoma ◽  
Epstein Barr Virus ◽  
Interleukin 10 ◽  
Epstein Barr Virus Infection ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Barr Virus ◽  
Il10 Gene ◽  
Epstein Barr

Abstract Context.—Hodgkin lymphoma is a neoplastic disease in which the immune system plays a major role in its pathogenesis. Interleukin 10 (IL-10), an immunosuppressive cytokine actively produced in patients with Hodgkin lymphomas, favors the survival of the Hodgkin/Reed-Sternberg cells. Individual variations in IL-10 levels may be due, in part, to the presence of single nucleotide polymorphisms in the IL10 gene promoter. Objective.—To evaluate whether particular single nucleotide polymorphisms in the IL10 gene are found more frequently in Hodgkin lymphoma cases associated with Epstein-Barr virus infection. Design.—The identification of single nucleotide polymorphisms at positions -1082 and -819/-592 in the IL10 gene was performed by polymerase chain reaction and restriction length fragment polymorphisms analysis in 65 cases of Hodgkin lymphoma and 50 cases of reactive benign follicular lymphoid hyperplasia (non-Hodgkin lymphoma control group). Results.—The frequency of the genotype GG at position -1082 was found to be significantly higher in patients with Epstein-Barr virus–positive Hodgkin lymphoma compared with Epstein-Barr virus–negative cases. Conclusions.—The results suggest that the presence of specific single nucleotide polymorphisms in the IL10 gene, notably those associated with high IL-10 production, may play a role in the susceptibility to Epstein-Barr virus–positive Hodgkin lymphoma development.

scholarly journals Identification of Predictive Pathways for Non-Hodgkin Lymphoma Prognosis

2010 ◽  
Vol 9 ◽  
pp. CIN.S6315 ◽  
Author(s):  
Xuesong Han ◽  
Yang Li ◽  
Jian Huang ◽  
Yawei Zhang ◽  
Theodore Holford ◽  
...  
Keyword(s):  
Hodgkin Lymphoma ◽  
Predictive Power ◽  
B Cell Lymphoma ◽  
Separate Analysis ◽  
Nucleotide Polymorphisms ◽  
Clinical Factors ◽  
Single Nucleotide ◽  
Kegg Pathways ◽  
Non Hodgkin Lymphoma ◽  
Individual Snps

Despite decades of intensive research, NHL (non-Hodgkin lymphoma) still remains poorly understood and is largely incurable. Recent molecular studies suggest that genomic variants measured with SNPs (single nucleotide polymorphisms) in genes may have additional predictive power for NHL prognosis beyond clinical risk factors. We analyzed a genetic association study. The prognostic cohort consisted of 346 patients, among whom 138 had DLBCL (diffuse large B-cell lymphoma) and 101 had FL (follicular lymphoma). For DLBCL, we analyzed 1229 SNPs which represented 122 KEGG pathways. For FL, we analyzed 1228 SNPs which represented 122 KEGG pathways. Unlike in existing studies, we targeted at identifying pathways with significant additional predictive power beyond clinical factors. In addition, we accounted for the joint effects of multiple SNPs within pathways, whereas some existing studies drew pathway-level conclusions based on separate analysis of individual SNPs. For DLBCL, we identified four pathways, which, combined with the clinical factors, had medians of the prediction logrank statistics as 2.535, 2.220, 2.094, 2.453, and 2.512, respectively. As a comparison, the clinical factors had a median of the prediction logrank statistics around 0.552. For FL, we identified two pathways, which, combined with the clinical factors, had medians of the prediction logrank statistics as 4.320 and 3.532, respectively. As a comparison, the clinical factors had a median of the prediction logrank statistics around 1.212. For NHL overall, we identified three pathways, which, combined with the clinical factors, had medians of the prediction logrank statistics as 5.722, 5.314, and 5.441, respective. As a comparison, the clinical factors had a median of the prediction logrank statistics around 4.411. The identified pathways have sound biological bases. In addition, they are different from those identified using existing approaches. They may provide further insights into the biological mechanisms underlying the prognosis of NHL.

scholarly journals Single nucleotide polymorphisms of interleukins associated with hepatitis C virus infection in Egypt

2017 ◽  
Vol 11 (03) ◽  
pp. 261-268 ◽  
Author(s):  
Hany Khalil ◽  
Mohamed Arfa ◽  
Samir El-Masrey ◽  
Sherif M EL-Sherbini ◽  
Amal A Abd-Elaziz
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Single Nucleotide Polymorphisms ◽  
Critical Role ◽  
Interleukin 10 ◽  
High Rate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Mutant Genotype

Introduction: Hepatitis C is a liver disease caused by the hepatitis C virus (HCV). It can cause both acute and chronic hepatitis infection. Based on secretion of required cytokines upon infection, HCV can improve its own RNA and successfully complete the replication cycle. Importantly, single nucleotide polymorphisms (SNPs) are the most common type of genetic variation and have been found to play a critical role in modulation of cellular cytokine production and interaction. Methodology: A total of 100 blood samples were obtained from HCV patients, and 120 samples were obtained from healthy individuals who served as controls. SNPs of interleukin-10/592 (IL-10/592) and IL-4/589 were investigated for possible connection with HCV infection. Relative expression of IL-4, IL-6, and IL-10 were detected using real-time polymerase chain reaction and enzyme-linked immunosorbent assay Results: The polymorphisms of IL-10 revealed a high rate of mutant genotype CC within the location IL-10/592 in HCV patients and controls, which resulted in low secretion of IL-10. Interestingly, the findings here demonstrate a positive association between HCV load of viremia and the mutant genotype IL-4-589/TT accompanied with low expression IL-4 in comparison with IL-6 expression. Conclusions: These data suggest that the expression of IL-4 is inversely proportional to HCV load of viremia, and this connection is due to the high level of mutant genotype IL-4-589/TT in infected patients located in gene promoter and inhibits gene expression.  

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