Reactive oxygen species (ROS) production in human peripheral blood neutrophils exposedin vitroto static magnetic field

2013 ◽  
Vol 32 (4) ◽  
pp. 560-568 ◽  
Author(s):  
Barbara Poniedziałek ◽  
Piotr Rzymski ◽  
Jacek Karczewski ◽  
Feliks Jaroszyk ◽  
Krzysztof Wiktorowicz
Keyword(s):  
Magnetic Field ◽  
Reactive Oxygen Species ◽  
Static Magnetic Field ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Reactive Oxygen ◽  
Ros Production ◽  
Oxygen Species ◽  
Blood Neutrophils

scholarly journals Reactive Oxygen Species Production in Peripheral Blood Neutrophils of Obstructive Sleep Apnea Patients

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Guoda Pilkauskaite ◽  
Skaidrius Miliauskas ◽  
Raimundas Sakalauskas
Keyword(s):  
Reactive Oxygen Species ◽  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Peripheral Blood ◽  
Reactive Oxygen ◽  
Apnea Hypopnea Index ◽  
Ros Production ◽  
Oxygen Species ◽  
Obstructive Sleep ◽  
Blood Neutrophils

Obstructive sleep apnea (OSA) as well as obesity is associated with increased production of reactive oxygen species (ROS). Neutrophils produce great amounts of ROS. The aim was to evaluate peripheral blood neutrophils ROS production in men with OSA and to establish relations with disease severity and obesity.Methods. Forty-six men with OSA and 10 controls were investigated. OSA was confirmed by polysomnography (PSG), when apnea/hypopnea index was >5/h. Body mass index (BMI) was evaluated. Neutrophils were isolated from peripheral blood in the morning after PSG. Dihydrorhodamine-123 was used for ROS detection. Data is presented as median (25th and 75th percentiles). All subjects were divided into four groups: nonobese mild-to-moderate OSA, obese mild-to-moderate OSA, nonobese severe OSA, and obese severe OSA.Results. Neutrophil ROS production was higher in nonobese severe OSA group compared to nonobese mild-to-moderate OSA (mean fluorescence intensity (MFI) 213.4 (89.0–238.9) versus 44.5 (20.5–58.4),P<0.05). In obese patient groups, ROS production was more increased in severe OSA compared to mild-to-moderate OSA group (MFI 74.5 (47.9–182.4) versus 31.0 (14.8–53.8),P<0.05). It did not differ in the groups with different BMI and the same severity of OSA.Conclusion. Increased neutrophil ROS production was related to more severe OSA but not obesity.

The production of reactive oxygen species in peripheral blood neutrophils is modulated by airway mucous

Open Medicine ◽  
2009 ◽  
Vol 4 (2) ◽  
pp. 245-252 ◽  
Author(s):  
Agne Babusyte ◽  
Jolanta Jeroch ◽  
Rimantas Stakauskas ◽  
Raimundas Sakalauskas
Keyword(s):  
Reactive Oxygen Species ◽  
Peripheral Blood ◽  
Reactive Oxygen ◽  
Lavage Fluid ◽  
Chronic Obstructive ◽  
Ros Generation ◽  
Ros Production ◽  
Oxygen Species ◽  
Copd Patients ◽  
Blood Neutrophils

AbstractNeutrophils are a major source of reactive oxygen species (ROS). The role of airway mucous on ROS production is unknown. The aim of our study was to investigate the direct influence of bronchoalveolar lavage fluid (BALF) and induced sputum (IS) alone or in combination with chemical/biological stimulus on ROS production in peripheral blood neutrophils during chronic obstructive pulmonary disease (COPD). Neutrophils were isolated from peripheral blood of 47 patients with moderate COPD and 14 healthy individuals (HI). BALF/RPMI (1:1) or IS/RPMI (1:1) from COPD patients were used to stimulate neutrophils alone or in combination with phorbolmyristate- acetate (PMA) (0.1–30 nM) or Staphylococcus aureus bacteria (0.7–500 bact/neutrophil). Relative generation of ROS was measured flow cytometrically. BALF/RPMI and in combination with relatively low PMA or all bacteria concentrations stimulated ROS; while, combination with relatively high PMA concentrations suppressed ROS in of COPD patients and HI. IS/RPMI and its combination with PMA inhibited ROS generation in both groups; whereas, IS stimulated or had a tendency to stimulate ROS production with relatively high bacteria concentrations. In conclusion, BALF and IS directly or in combination with chemical/biological factors modulated ROS production. This effect was stronger in neutrophils from COPD patients and depended on chemical/biological stimulus intensity.

The involvement of TLR2 in cytokine and reactive oxygen species (ROS) production by PBMCs in response toLeishmania majorphosphoglycans (PGs)

Parasitology ◽  
2009 ◽  
Vol 136 (10) ◽  
pp. 1193-1199 ◽  
Author(s):  
G. KAVOOSI ◽  
S. K. ARDESTANI ◽  
A. KARIMINIA
Keyword(s):  
Reactive Oxygen Species ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Reactive Oxygen ◽  
Ros Production ◽  
Oxygen Species ◽  
Peripheral Blood Mononuclear ◽  
Cytokine Milieu ◽  
Antigen Presenting ◽  
First Time

SUMMARYIn the present study, we show for the first time that lipophosphoglycan (LPG) stimulated cytokine production by human peripheral blood mononuclear cells is also mediated via Toll-like receptor (TLR2). In addition, in order to verify if TLR2 is involved in recognition of the purified PGs, neutralizing mAbs against TLR2 and TLR4 were used to treat the cells before being stimulated with PGs. We found strong Th1-promoting cytokines induced by sLPG but not by mLPG which was blocked by presence of anti-TLR2 mAb. This finding reveals a mechanism by which the first encounter and recognition ofL. majorpromastigotes by mLPG after interaction with TLR2 provides a cytokine milieu for consequent Th2 differentiation. Moreover, having shown the strong induction of Th1-promoting cytokines and low production of IL-10 in response to sLPG might have vaccine implication since it is recognized by TLR2 providing signals to professional antigen presenting cells that reside in the skin to promote effective T cell responses againstLeishmaniainfection. In addition, it was shown that purified mLPG and sLPG activate reactive oxygen species (ROS) production which is also blocked by anti-TLR2 but not by anti-TLR4. However, no inhibition was seen in PPG-induced cytokine and ROS production in the presence of anti-TLR2 and anti-TLR4, implying involvement of other receptors.

Reactive oxygen species activate human peripheral blood dendritic cells

1999 ◽  
Vol 26 (1-2) ◽  
pp. 232-238 ◽  
Author(s):  
Karine Rutault ◽  
Charles Alderman ◽  
Benjamin M. Chain ◽  
David R. Katz
Keyword(s):  
Reactive Oxygen Species ◽  
Dendritic Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Reactive Oxygen ◽  
Oxygen Species

Reactive Oxygen Species Are Differentially Regulated in Chronic Myeloid Leukemia Versus Philadelphia Negative Myeloproliferative Neoplasms

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 4215-4215
Author(s):  
Estelle Guerin ◽  
Francis Belloc ◽  
Gabriel Etienne ◽  
Pierre Duffau ◽  
Francois-Xavier Mahon ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Bone Marrow ◽  
Peripheral Blood ◽  
Myeloproliferative Neoplasms ◽  
Reactive Oxygen ◽  
Ros Generation ◽  
Separate Analysis ◽  
Ros Production ◽  
Oxygen Species ◽  
Normal Cells

Abstract Deregulation of tyrosine-kinases is a characteristic of most Myeloproliferative Neoplasms (MPN); evolution from chronic phase to acute leukemia depends on the acquisition of additional mutations. Reactive Oxygen Species (ROS), the production of which is increased by tyrosine-kinase activation, can be responsible for additional mutations. The role of ROS in generating genetic aberrations has been mainly studied in BCR-ABL-positive cell lines. Little is known of ROS metabolism in primary cells from CML or Philadelphia-negative MPN (Ph-MPN). After informed consent, cells from blood or bone marrow were obtained from patients diagnosed with CML (12 bone marrow (BM), 8 peripheral blood (PB)), or Ph-MPN (4 Polycythemia Vera, 6 Essential Thrombocythemia, 3 Primary Myelofibroses, 2 atypical CML) and from healthy donors (bone marrow donors) or patients devoid of hematological disease undergoing thoracotomy. Cells were incubated with DCFDA, a fluorogenic marker of ROS production, labelled with an anti-CD45 antibody, stimulated with either the oxidant hydrogen peroxide (H2O2) or the PKC activator Phorbol Myristate Acetate (PMA), and analysed for ROS production by flow cytometry. CD45/SSC gating allowed separate analysis of granulocytes, monocytes or lymphocytes. The basal level of ROS was not higher in CML cells as compared to normal BM or PB leukocytes. It was even significantly lower in CML lymphocytes, either from the BM (2.35 Arbitrary Units vs 8.3 AU, p=5.5 10−5) or PB (2.47 AU vs 7.4 AU, p=3.10−5) and in CML granulocytes from peripheral blood (14 AU vs 45 AU, p =10 −5), but not bone marrow. The ROS levels of Ph-MPN cells were similar or slightly higher than control cells. Upon H2O2 stimulation however, ROS production increased significantly more in CML cells as compared to normal cells (6 fold increase), whatever the cell type (granulocytes, monocytes and lymphocytes) or their origin (PB or BM). In contrast, for Ph-MPN cells, H2O2-stimulated ROS production was close to that of normal cells, with only BM lymphocytes showing ROS generation four fold higher than control BM lymphocytes. After PMA stimulation, which yielded a more modest ROS production than H2O2, CML cells behaved similarly to normal cells, whereas ROS production was four fold higher in Ph-MPN cells, whatever their type and origin. In conclusion, ROS levels at the basal stage are not higher in MPN cells, whether they are Philadelphia positive or negative, as compared to normal cells. Various kinds of stimulation induce different patterns of response, CML cells being more sensitive to oxidants whereas Ph-MPN cells respond more to the cytokine-mimicking agent PMA. These results suggest that the mechanisms of ROS generation and thus of genetic instability are different in CML and Ph-MPN.

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