scholarly journals Ingestion of Tabebuia avellanedae (Taheebo) Inhibits Production of Reactive Oxygen Species from Human Peripheral Blood Neutrophils

2015 ◽  
pp. 1-4
Author(s):  
Ohno S ◽  
◽  
Ohno Y ◽  
Suzuki Y ◽  
Miura S ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Blood Neutrophils

Reactive oxygen species activate human peripheral blood dendritic cells

1999 ◽  
Vol 26 (1-2) ◽  
pp. 232-238 ◽  
Author(s):  
Karine Rutault ◽  
Charles Alderman ◽  
Benjamin M. Chain ◽  
David R. Katz
Keyword(s):  
Reactive Oxygen Species ◽  
Dendritic Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Reactive Oxygen Species Production in Peripheral Blood Neutrophils of Obstructive Sleep Apnea Patients

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Guoda Pilkauskaite ◽  
Skaidrius Miliauskas ◽  
Raimundas Sakalauskas
Keyword(s):  
Reactive Oxygen Species ◽  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Peripheral Blood ◽  
Reactive Oxygen ◽  
Apnea Hypopnea Index ◽  
Ros Production ◽  
Oxygen Species ◽  
Obstructive Sleep ◽  
Blood Neutrophils

Obstructive sleep apnea (OSA) as well as obesity is associated with increased production of reactive oxygen species (ROS). Neutrophils produce great amounts of ROS. The aim was to evaluate peripheral blood neutrophils ROS production in men with OSA and to establish relations with disease severity and obesity.Methods. Forty-six men with OSA and 10 controls were investigated. OSA was confirmed by polysomnography (PSG), when apnea/hypopnea index was >5/h. Body mass index (BMI) was evaluated. Neutrophils were isolated from peripheral blood in the morning after PSG. Dihydrorhodamine-123 was used for ROS detection. Data is presented as median (25th and 75th percentiles). All subjects were divided into four groups: nonobese mild-to-moderate OSA, obese mild-to-moderate OSA, nonobese severe OSA, and obese severe OSA.Results. Neutrophil ROS production was higher in nonobese severe OSA group compared to nonobese mild-to-moderate OSA (mean fluorescence intensity (MFI) 213.4 (89.0–238.9) versus 44.5 (20.5–58.4),P<0.05). In obese patient groups, ROS production was more increased in severe OSA compared to mild-to-moderate OSA group (MFI 74.5 (47.9–182.4) versus 31.0 (14.8–53.8),P<0.05). It did not differ in the groups with different BMI and the same severity of OSA.Conclusion. Increased neutrophil ROS production was related to more severe OSA but not obesity.

The production of reactive oxygen species in peripheral blood neutrophils is modulated by airway mucous

Open Medicine ◽  
2009 ◽  
Vol 4 (2) ◽  
pp. 245-252 ◽  
Author(s):  
Agne Babusyte ◽  
Jolanta Jeroch ◽  
Rimantas Stakauskas ◽  
Raimundas Sakalauskas
Keyword(s):  
Reactive Oxygen Species ◽  
Peripheral Blood ◽  
Reactive Oxygen ◽  
Lavage Fluid ◽  
Chronic Obstructive ◽  
Ros Generation ◽  
Ros Production ◽  
Oxygen Species ◽  
Copd Patients ◽  
Blood Neutrophils

AbstractNeutrophils are a major source of reactive oxygen species (ROS). The role of airway mucous on ROS production is unknown. The aim of our study was to investigate the direct influence of bronchoalveolar lavage fluid (BALF) and induced sputum (IS) alone or in combination with chemical/biological stimulus on ROS production in peripheral blood neutrophils during chronic obstructive pulmonary disease (COPD). Neutrophils were isolated from peripheral blood of 47 patients with moderate COPD and 14 healthy individuals (HI). BALF/RPMI (1:1) or IS/RPMI (1:1) from COPD patients were used to stimulate neutrophils alone or in combination with phorbolmyristate- acetate (PMA) (0.1–30 nM) or Staphylococcus aureus bacteria (0.7–500 bact/neutrophil). Relative generation of ROS was measured flow cytometrically. BALF/RPMI and in combination with relatively low PMA or all bacteria concentrations stimulated ROS; while, combination with relatively high PMA concentrations suppressed ROS in of COPD patients and HI. IS/RPMI and its combination with PMA inhibited ROS generation in both groups; whereas, IS stimulated or had a tendency to stimulate ROS production with relatively high bacteria concentrations. In conclusion, BALF and IS directly or in combination with chemical/biological factors modulated ROS production. This effect was stronger in neutrophils from COPD patients and depended on chemical/biological stimulus intensity.

scholarly journals Reactive Oxygen Species Formation and Apoptosis in Human Peripheral Blood Mononuclear Cell Induced by 900 MHz Mobile Phone Radiation

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Yao-Sheng Lu ◽  
Bao-Tian Huang ◽  
Yao-Xiong Huang
Keyword(s):  
Reactive Oxygen Species ◽  
Peripheral Blood Mononuclear Cell ◽  
Mononuclear Cell ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Reactive Oxygen ◽  
Antioxidant Defenses ◽  
Oxygen Species ◽  
Peripheral Blood Mononuclear ◽  
Human Pbmc

We demonstrate that reactive oxygen species (ROS) plays an important role in the process of apoptosis in human peripheral blood mononuclear cell (PBMC) which is induced by the radiation of 900 MHz radiofrequency electromagnetic field (RFEMF) at a specific absorption rate (SAR) of~0.4 W/kg when the exposure lasts longer than two hours. The apoptosis is induced through the mitochondrial pathway and mediated by activating ROS and caspase-3, and decreasing the mitochondrial potential. The activation of ROS is triggered by the conformation disturbance of lipids, protein, and DNA induced by the exposure of GSM RFEMF. Although human PBMC was found to have a self-protection mechanism of releasing carotenoid in response to oxidative stress to lessen the further increase of ROS, the imbalance between the antioxidant defenses and ROS formation still results in an increase of cell death with the exposure time and can cause about 37% human PBMC death in eight hours.

scholarly journals cAMP Activates The generation of Reactive Oxygen Species and Inhibits The Secretion of IL-6 in Peripheral Blood Mononuclear Cells from Type 2 Diabetic Patients

2009 ◽  
Vol 2 (5) ◽  
pp. 317-321 ◽  
Author(s):  
Camila Armond Isoni ◽  
Érica Abreu Borges ◽  
Clara Araújo Veloso ◽  
Rafael Teixeira Mattos ◽  
Miriam Martins Chaves ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
In Cells

Peripheral blood mononuclear cells (PBMNC) from patients with type 2 diabetes (DM2) have generated higher levels of reactive oxygen species (ROS) that were higher than those in cells from healthy individuals. In the presence of a cAMP-elevating agent, ROS production was significantly activated in PBMNC from DM2 patients but it was inhibited in cells from healthy subjects. Higher levels of IL-6 has been detected in the supernatant of PBMNC cultures from DM2 patients in comparison with healthy controls. When cells were cultured in the presence of a cAMP-elevating agent, the level of IL-6 decreased has by 46% in the supernatant of PBMNC from DM2 patients but it remained unaltered in controls. No correlations between ROS and IL-6 levels in PBMNC from DM2 patients or controls have been observed. Secretions of IL-4 or IFN by PBMNC from patients or controls have not been affected by the elevation of cAMP. cAMP elevating agents have activated the production of harmful reactive oxidant down modulated IL-6 secretion by these cells from DM2 patients, suggesting an alteration in the metabolic response possibly due to hyperglicemia. The results suggest that cAMP may play an important role in the pathogenesis of diabetes.

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