scholarly journals Composition of Airway Surface Liquid Determined by X-ray Microanalysis

2006 ◽  
Vol 111 (1) ◽  
pp. 137-153 ◽  
Author(s):  
Inna Kozlova ◽  
Viengphet Vanthanouvong ◽  
Johannesson Marie ◽  
Godfried M. Roomans
Keyword(s):  
Airway Surface Liquid ◽  
X Ray ◽  
Surface Liquid

Elemental Composition of Airway Surface Liquid in the Pig Determined by X-Ray Microanalysis

2005 ◽  
Vol 32 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Inna Kozlova ◽  
Viengphet Vanthanouvong ◽  
Birgitta Almgren ◽  
Marieann Högman ◽  
Godfried M. Roomans
Keyword(s):  
Elemental Composition ◽  
Airway Surface Liquid ◽  
X Ray ◽  
Surface Liquid

A Comparison of the Elemental Concentrations Obtained using X-ray Microanalysis of Bulk Cryofractured and Ultrathin Cryosectioned Mucus Layer of the Airway Surface Liquid in Mouse Trachea

2001 ◽  
Vol 7 (S2) ◽  
pp. 998-999
Author(s):  
C.A. Ackerley ◽  
G. Kent ◽  
Y.M. Heng ◽  
A. Tilups ◽  
L.E. Becker
Keyword(s):  
Thin Film ◽  
Filter Paper ◽  
Airway Surface Liquid ◽  
Mucus Layer ◽  
X Ray ◽  
Major Function ◽  
Cellular Debris ◽  
Surface Liquid ◽  
The Individual ◽  
Pathological Material

The airways are lined with a thin film consisting of two layers. A watery layer, the airway surface liquid (ASL) surrounds the cilia and its major function is to serve as a medium for ciliary boating of mucus out of the respiratory system. The other layer is a viscous mucus layer consisting mainly of airway cellular secretions and cellular debris.The composition and effects of altered concentrations of the constitutive elements in the ASL and mucus layer remain a mystery. in human patients, attempts have been made to determine the composition of these layers by sampling with a filter paper by touching it to the mucosa and wetting it by capillary action. This material was then removed from the filter paper and quantitative analyses using energy dispersive x-ray spectrometry (EDS) and a Hall’s thin film correction routine performed on the residue. Although differences were detected between normal and pathological material, these results did not reflect the composition of the individual layers but an average of both.Attempts have been made to determine the elemental composition of these layers in intact and in cultured rabbit trachea, bovine trachea and hamster trachea.

scholarly journals Measurements of airway surface liquid height and mucus transport by fluorescence microscopy, and of ion composition by X-ray microanalysis

2004 ◽  
Vol 3 ◽  
pp. 135-139 ◽  
Author(s):  
Godfried M. Roomans ◽  
Inna Kozlova ◽  
Harriet Nilsson ◽  
Viengphet Vanthanouvong ◽  
Brian Button ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Airway Surface Liquid ◽  
Ion Composition ◽  
X Ray ◽  
Liquid Height ◽  
Surface Liquid

Collection and X-ray microanalysis of airway surface liquid in the mouse using ion exchange beads

Micron ◽  
2004 ◽  
Vol 35 (8) ◽  
pp. 701-705 ◽  
Author(s):  
Harriet Nilsson ◽  
Inna Kozlova ◽  
Viengphet Vanthanouvong ◽  
Godfried M Roomans
Keyword(s):  
Ion Exchange ◽  
Airway Surface Liquid ◽  
X Ray ◽  
Surface Liquid

X‐ray microanalysis of native airway surface liquid collected by cryotechnique

1998 ◽  
Vol 191 (3) ◽  
pp. 311-319 ◽  
Author(s):  
Baconnais ◽  
Zahm ◽  
Kilian ◽  
Bonhomme ◽  
Gobillard ◽  
...  
Keyword(s):  
Airway Surface Liquid ◽  
X Ray ◽  
Surface Liquid

X-ray microanalysis of airway surface liquid in the mouse

2005 ◽  
Vol 288 (5) ◽  
pp. L874-L878 ◽  
Author(s):  
Inna Kozlova ◽  
Harriet Nilsson ◽  
Mia Phillipson ◽  
Brigitte Riederer ◽  
Ursula Seidler ◽  
...  
Keyword(s):  
Elemental Composition ◽  
Ionic Composition ◽  
Airway Surface Liquid ◽  
X Ray ◽  
Relative Paucity ◽  
Wide Range ◽  
Surface Liquid ◽  
Bead Method ◽  
Range Of Values

The ionic composition of airway surface liquid (ASL) has been debated, and, in particular for the mouse, a wide range of values has been published. Two techniques were developed to measure the elemental composition of the ASL. X-ray microanalysis of ASL was carried out at low temperature on trachea removed from isoflurane-anesthetized animals and shock-frozen. In the second technique, dextran beads were placed on top of the epithelium of the trachea removed from pentobarbital-anesthetized animals, left to equilibrate with the ASL, dried, and subjected to X-ray microanalysis. Both techniques showed that mouse tracheal ASL has significantly lower concentrations of Na and Cl (∼60–80 mM) than serum. Differences between the two techniques were due to different sampling of mucus. CFTR(−/−) mice had significantly higher concentrations of Na and Cl in their ASL than age-matched controls. Pilocarpine or isoproterenol stimulation significantly reduced the ion concentrations in tracheal ASL. ASL was also collected with the dextran bead method from the nasal cavity in situ in pentobarbital-anesthetized animals. In control animals, the elemental composition of nasal fluid was similar to that of tracheal ASL. Pilocarpine stimulation caused a significant increase in Na, Cl, and K; stimulation with isoproterenol or phenylephrine caused a significant increase only in K. It is concluded that mouse ASL under unstimulated conditions is hypotonic, which may be related to the relative paucity of submucosal glands in the mouse trachea.

X-ray microanalysis of airway surface liquid collected in cystic fibrosis mice

2001 ◽  
Vol 281 (2) ◽  
pp. L309-L313 ◽  
Author(s):  
Jean-Marie Zahm ◽  
Sonia Baconnais ◽  
Donald J. Davidson ◽  
Sheila Webb ◽  
Julia Dorin ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Dry Weight ◽  
Surface Epithelium ◽  
Airway Surface Liquid ◽  
Key Factors ◽  
Ion Content ◽  
Transport Efficiency ◽  
X Ray ◽  
The Mean ◽  
Surface Liquid

The airway surface liquid (ASL) that lines the airway surface epithelium plays a major role in airway antibacterial defense and mucociliary transport efficiency, two key factors in cystic fibrosis (CF) disease. A major difficulty is to collect ASL in native conditions without stimulation or alteration of the underlying airway epithelium. Using a cryoprobe specifically adapted to collect native ASL from the tracheal mouse surface, we analyzed by X-ray microanalysis the complete ASL and plasma ion content in Cftrtm1Hgu/Cftrtm1Hgu mice compared with that in control littermates. ASL ion content from eight Cftrtm1Hgu/Cftrtm1Hgu mice and eight control littermates did not appear significantly different. The mean (±SE) concentrations were 2,352 ± 367 and 2,058 ± 401 mmol/kg dry weight for Na, 1,659 ± 272 and 1,448 ± 281 mmol/kg dry weight for Cl, 357 ± 57 and 337 ± 38 mmol/kg dry weight for S, 1,066 ± 220 and 787 ± 182 mmol/kg dry weight for K, 400 ± 82 and 301 ± 58 mmol/kg dry weight for Ca, 105 ± 31 and 105 ± 20 mmol/kg dry weight for Mg, 33 ± 15 and 29 ± 9 mmol/kg dry weight for P in non-CF and CF mice, respectively. This cryotechnique appears to be a promising technique for analyzing the complete elemental composition of native ASL in CF and non-CF tissues.

scholarly journals Measuring Airway Surface Liquid Depth in Ex Vivo Mouse Airways by X-Ray Imaging for the Assessment of Cystic Fibrosis Airway Therapies

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e55822 ◽  
Author(s):  
Kaye S. Morgan ◽  
Martin Donnelley ◽  
David M. Paganin ◽  
Andreas Fouras ◽  
Naoto Yagi ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Ex Vivo ◽  
Airway Surface Liquid ◽  
X Ray ◽  
X Ray Imaging ◽  
Cystic Fibrosis Airway ◽  
Liquid Depth ◽  
Surface Liquid

Ionic composition of rat airway surface liquid determined by X-ray microanalysis

2005 ◽  
Vol 68 (1) ◽  
pp. 6-12 ◽  
Author(s):  
Viengphet Vanthanouvong ◽  
Inna Kozlova ◽  
Godfried M. Roomans
Keyword(s):  
Ionic Composition ◽  
Airway Surface Liquid ◽  
X Ray ◽  
Surface Liquid
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