scholarly journals Gene expression and characterization of a novel GH family 18 chitinase from extremely halophilic archaeon Halobacterium salinarum NRC-1

2010 ◽  
Vol 9 (1) ◽  
pp. 19-24 ◽  
Author(s):  
R Yatsunami ◽  
M Sato ◽  
K Orishimo ◽  
Y Hatori ◽  
Y Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Halobacterium Salinarum ◽  
Halophilic Archaeon ◽  
Extremely Halophilic Archaeon

scholarly journals Construction and Characterization of a Gradually Inducible Expression Vector for Halobacterium salinarum, Based on thekdpPromoter

2012 ◽  
Vol 78 (7) ◽  
pp. 2100-2105 ◽  
Author(s):  
Dorthe Kixmüller ◽  
Jörg-Christian Greie
Keyword(s):  
Gene Expression ◽  
Expression Vector ◽  
Expression System ◽  
Expression Patterns ◽  
Inducible Expression ◽  
Halobacterium Salinarum ◽  
Expression Vectors ◽  
Content Type ◽  
Inducible Expression System

ABSTRACTGradually inducible expression vectors which are governed by variations of growth conditions are powerful tools for gene expression of conditionally lethal mutants. Furthermore, controlled expression allows monitoring of overproduction of proteins at various stages in their expressing hosts. ForHalobacterium salinarum, which is often used as a paradigm for halophilic archaea, such an inducible expression system is not available to date. Here we show that thekdppromoter (Pkdp), which facilitates gene expression upon K+limitation, can be used to establish such a system for molecular applications. Pkdpfeatures a rather high expression rate, with an approximately 50-fold increase that can be easily varied by K+concentrations in the growth medium. Besides the construction of an expression vector, our work describes the characterization of expression patterns and, thus, offers a gradually inducible expression system to the scientific community.

Purification and partial characterization of cell surface glycoprotein from extremely halophilic archaeon Haloarcula japonica strain TR-1

1995 ◽  
Vol 17 (7) ◽  
pp. 705-706 ◽  
Author(s):  
Satoshi Nakamura ◽  
Shin Mizutani ◽  
Hiromichi Wakai ◽  
Hiroshi Kawasaki ◽  
Rikizo Aono ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Glycoprotein ◽  
Partial Characterization ◽  
Halophilic Archaeon ◽  
Cell Surface Glycoprotein ◽  
Haloarcula Japonica ◽  
Extremely Halophilic Archaeon

Characterization of 3-isopropylmalate dehydrogenase from extremely halophilic archaeon Haloarcula japonica

2021 ◽  
Author(s):  
Shintaro Nagaoka ◽  
Noriko Sugiyama ◽  
Rie Yatsunami ◽  
Satoshi Nakamura
Keyword(s):  
Analytical Ultracentrifugation ◽  
Maximum Activity ◽  
Oxidative Decarboxylation ◽  
Halophilic Archaeon ◽  
Leucine Biosynthesis ◽  
First Report ◽  
Haloarcula Japonica ◽  
Extremely Halophilic Archaeon ◽  
Isopropylmalate Dehydrogenase

Abstract 3-Isopropylmalate dehydrogenase (IPMDH) catalyzes oxidative decarboxylation of (2R, 3S)-3-isopropylmalate to 2-oxoisocaproate in leucine biosynthesis. In this study, recombinant IPMDH (HjIPMDH) from an extremely halophilic archaeon, Haloarcula japonica TR-1, was characterized. Activity of HjIPMDH increased as KCl concentration increased, and the maximum activity was observed at 3.0 M KCl. Analytical ultracentrifugation revealed that HjIPMDH formed a homotetramer at high KCl concentrations, and it dissociated to a monomer at low KCl concentrations. Additionally, HjIPMDH was thermally stabilized by higher KCl concentrations. This is the first report on haloarchaeal IPMDH.

scholarly journals Gene Analysis, Expression, and Characterization of an Intracellular α-Amylase from the Extremely Halophilic Archaeon Haloarcula japonica

2013 ◽  
Vol 77 (2) ◽  
pp. 281-288 ◽  
Author(s):  
Masahiko ONODERA ◽  
Rie YATSUNAMI ◽  
Wataru TSUKIMURA ◽  
Toshiaki FUKUI ◽  
Kaoru NAKASONE ◽  
...  
Keyword(s):  
Gene Analysis ◽  
Halophilic Archaeon ◽  
Haloarcula Japonica ◽  
Extremely Halophilic Archaeon

scholarly journals NaCl-activated nucleoside diphosphate kinase from extremely halophilic archaeon, Halobacterium salinarum , maintains native conformation without salt

FEBS Letters ◽  
2001 ◽  
Vol 493 (2-3) ◽  
pp. 134-138 ◽  
Author(s):  
Matsujiro Ishibashi ◽  
Hiroko Tokunaga ◽  
Kazushi Hiratsuka ◽  
Yasushi Yonezawa ◽  
Hirohito Tsurumaru ◽  
...  
Keyword(s):  
Nucleoside Diphosphate Kinase ◽  
Halobacterium Salinarum ◽  
Halophilic Archaeon ◽  
Native Conformation ◽  
Extremely Halophilic Archaeon

Characterization of a GlgC homolog from extremely halophilic archaeon Haloarcula japonica

2021 ◽  
Author(s):  
Rin Sueda ◽  
Kento Yoshida ◽  
Masahiko Onodera ◽  
Toshiaki Fukui ◽  
Rie Yatsunami ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Glycogen Synthesis ◽  
Recombinant Enzyme ◽  
Halophilic Archaeon ◽  
Haloarcula Japonica ◽  
Extremely Halophilic Archaeon

ABSTRACT Glycogen synthesis in bacteria is mainly organized by the products of glgB, glgC, and glgA genes comprising the widely known glg operon. On the genome of extremely halophilic archaeon Haloarcula japonica, there was a gene cluster analogous to the bacterial glg operon. In this study, we focused on a GlgC homolog of Ha. japonica, and its recombinant enzyme was prepared and characterized. The enzyme showed highest activity toward GTP and glucose-1-phosphate as substrates in the presence of 2.6 m KCl and predicted to be work as “GDP-glucose pyrophosphorylase” in Ha. japonica.

scholarly journals The extremely halophilic archaeon Halobacterium salinarum ETD5 from the solar saltern of Sfax (Tunisia) produces multiple halocins

2020 ◽  
Vol 171 (2) ◽  
pp. 80-90 ◽  
Author(s):  
Fadoua Ghanmi ◽  
Alyssa Carré-Mlouka ◽  
Zied Zarai ◽  
Hafedh Mejdoub ◽  
Jean Peduzzi ◽  
...  
Keyword(s):  
Halobacterium Salinarum ◽  
Solar Saltern ◽  
Halophilic Archaeon ◽  
Extremely Halophilic Archaeon
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