scholarly journals DETEKSI INFEKSI Human papillomavirus (HPV) 16/18 PADA KARSINOMA SEL SKUAMOSA RONGGA MULUT DENGAN NESTED PCR

2019 ◽  
Author(s):  
Siti Hamidatul Aliyah
Keyword(s):  
Head And Neck Cancer ◽  
Human Papillomavirus ◽  
Head And Neck ◽  
Neck Cancer ◽  
Cancer Incidence ◽  
Nested Pcr ◽  
Hpv Infection ◽  
Hpv 16 ◽  
Hpv E6 ◽  
Hpv 18

Head and neck cancer ranks fourth nationally cancer incidence in Indonesia. Oral SCC isone of Head and neck cancer incidence. Oral SCCrelated to several factors, includingsmoking, alcohol, viral infection human papillomavirus (HPV 16/18) and genetic On theother hand, HPV E6 oncoprotein binds and inactivates TP53, and result in loss of control ofthe cell cycle.This study aimed to detect HPV 16/18 infection in oral SCC. Detection of HPVserotypes 16 and 18 performed on FFPE DNA isolates oral SCC with the method of nestedPolymerase Chain Reaction (PCR). Nested PCR was performed in two stages, namelyamplification with L1 primer, followed by specific PCR E6 HPV-16 and HPV-18. A total of33% (11/33) FFPE samples showed positive for HPV 18 infection (single-sized DNA bands415bp) and not detected the presence of infection with HPV 16. It can be concluded that thetype of FFPE biosampel can be used for studies related to HPV infection. Furthermore, itshould be tested on different types biosampel by a larger amount so as to represent theprevalence of oncogenic HPV infection in Indonesia.

Human papillomavirus (HPV) 16 and the prognosis of head and neck cancer in a geographical region with a low prevalence of HPV infection

2014 ◽  
Vol 25 (4) ◽  
pp. 461-471 ◽  
Author(s):  
Rossana Verónica Mendoza López ◽  
José Eduardo Levi ◽  
José Eluf-Neto ◽  
Rosalina Jorge Koifman ◽  
Sergio Koifman ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Human Papillomavirus ◽  
Head And Neck ◽  
Neck Cancer ◽  
Hpv Infection ◽  
Geographical Region ◽  
Hpv 16 ◽  
Low Prevalence

scholarly journals Trends in head and neck cancer incidence in Denmark, 1978-2007: Focus on human papillomavirus associated sites

2010 ◽  
Vol 129 (3) ◽  
pp. 733-741 ◽  
Author(s):  
Maria Blomberg ◽  
Ann Nielsen ◽  
Christian Munk ◽  
Susanne Krüger Kjaer
Keyword(s):  
Head And Neck Cancer ◽  
Human Papillomavirus ◽  
Head And Neck ◽  
Neck Cancer ◽  
Cancer Incidence

EGFR mutations and human papillomavirus in squamous cell carcinoma of tongue and tonsil

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 10027-10027 ◽  
Author(s):  
I. Na ◽  
H. Kang ◽  
Y. Park ◽  
J. Koh ◽  
S. Cho ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Human Papillomavirus ◽  
Head And Neck ◽  
Neck Cancer ◽  
Egfr Mutation ◽  
Tongue Cancer ◽  
Disease Free Survival ◽  
Hpv Infection ◽  
Egfr Mutations ◽  
Tonsil Cancer

10027 Background: Some studies reported the presence of somatic mutation of EGFR in head and neck cancer. Human papillomavirus (HPV) was associated with some types of head and neck cancer. We performed this study to determine clinical significance of EGFR mutation and its association with HPV infection in patients with tongue and tonsil cancer. Methods: We sequenced exons 18–21 of EGFR TK domain and HPV typing was performed using the HPV DNA chip in tissues of patients with tongue and tonsil cancer. Univariate and multivariate analysis was performed. Results: Eighty-one patients were included. We detected EGFR mutation in 10 (12%) patients. Mutation was significantly frequent in never-smoker (21% versus 5%; P = 0.031). The mutation frequency did not differ between tongue and tonsil cancer (12% vs. 13%, respectively; P = 0.978). There was no difference between female and male (12% vs. 14%, respectively; P = 0.754). Eleven of 81 (14%) patients were HPV positive. HPV positivity was more common in young (<60 years) than older (≥60 years) patients (20% vs. 3%, respectively; P = 0.039). Patients with tonsil cancer showed more frequent HPV positivity than tongue cancer (42% vs. 2%, respectively; P < 0.001). There was no association between HPV positivity and EGFR mutation. In terms of disease-free survival, patients with EGFR mutation had better survival independently of stage and primary site (hazard ratio, 0.246; 95% CI, 0.075 to 0.802). Conclusions: We did not find association between EGFR mutation and HPV positivity. Patients with EGFR mutation showed better outcome in tongue and tonsil cancer. These results suggest that EGFR mutation may be a prognostic factor in patients with head and neck cancer. No significant financial relationships to disclose.

Risk factors and survival by HPV-16 E6 and E7 antibody status in human papillomavirus positive head and neck cancer

2009 ◽  
Vol 127 (1) ◽  
pp. 111-117 ◽  
Author(s):  
Elaine M. Smith ◽  
Michael Pawlita ◽  
Linda M. Rubenstein ◽  
Thomas H. Haugen ◽  
Eva Hamsikova ◽  
...  
Keyword(s):  
Risk Factors ◽  
Head And Neck Cancer ◽  
Human Papillomavirus ◽  
Head And Neck ◽  
Neck Cancer ◽  
Hpv 16 ◽  
E6 And E7

scholarly journals Role of Human Papillomavirus Infection in Head and Neck Cancer in Italy: The HPV-AHEAD Study

Cancers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3567
Author(s):  
Marta Tagliabue ◽  
Marisa Mena ◽  
Fausto Maffini ◽  
Tarik Gheit ◽  
Beatriz Quirós Blasco ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Human Papillomavirus ◽  
Head And Neck ◽  
Neck Cancer ◽  
Proportional Hazards ◽  
Disease Free Survival ◽  
Hpv Dna ◽  
Hpv E6 ◽  
Formalin Fixed Paraffin Embedded

Literature on the role of human papillomavirus (HPV) in head and neck cancer (HNC) in Italy is limited, especially for non-oropharyngeal tumours. Within the context of the HPV-AHEAD study, we aimed to assess the prognostic value of different tests or test algorithms judging HPV carcinogenicity in HNC and factors related to HPV positivity at the European Institute of Oncology. We conducted a retrospective cohort study (2000–2010) on a total of 696 primary HNC patients. Formalin-fixed, paraffin-embedded cancer tissues were studied. All HPV-DNA-positive and a random sample of HPV-DNA-negative cases were subjected to HPV-E6*I mRNA detection and p16INK4a staining. Multivariate models were used to assess for factors associated with HPV positivity and proportional hazards for survival and recurrence. The percentage of HPV-driven cases (considering HPV-E6*I mRNA positivity) was 1.8, 2.2, and 40.4% for oral cavity (OC), laryngeal (LC), and oropharyngeal (OPC) cases, respectively. The estimates were similar for HPV-DNA/p16INK4a double positivity. Being a non-smoker or former smoker or diagnosed at more recent calendar periods were associated with HPV-E6*I mRNA positivity only in OPC. Being younger was associated with HPV-E6*I mRNA positivity in LC. HPV-driven OPC, but not HPV-driven OC and LC, showed better 5 year overall and disease-free survival. Our data show that HPV prevalence in OPC was much higher than in OC and LC and observed to increase in most recent years. Moreover, HPV positivity conferred better prognosis only in OPC. Novel insights on the role of HPV in HNC in Italy are provided, with possible implications in the clinical management of these patients.

Head and neck cancer

2016 ◽  
pp. 329-364
Author(s):  
Christine H. Chung ◽  
Andreas Dietz ◽  
Vincent Gregoire ◽  
Marco Guzzo ◽  
Marc Hamoir ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Human Papillomavirus ◽  
High Risk ◽  
Molecular Biology ◽  
Head And Neck ◽  
Neck Cancer ◽  
Hpv Infection ◽  
Head And Neck Cancers ◽  
Patient Treatment ◽  
Genomic Analyses

This chapter discusses head and neck cancers, and covers the epidemiology and molecular biology of head and neck cancer. Head and neck cancer is a heterogeneous disease and most commonly caused by tobacco and alcohol use, as well as high-risk human papillomavirus (HPV) infection. HPV-negative and -positive HNSCC are demographically, biologically and clinically distinct entities with more favourable outcomes associated with HPV-positive tumours of the oropharynx. Comprehensive genomic analyses show that more functional loss of tumour suppressors are present in HPV-negative tumours compared to HPV-positive tumours. Furthermore, HNSCC can be molecularly characterized into five subtypes. The challenges facing future investigations are efficient translation of these biological findings into clinically meaningful advancements in patient treatment.

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