scholarly journals Molecular marker aided breeding for blast resistant rice in Nepal

2014 ◽  
Vol 15 ◽  
pp. 128-138
Author(s):  
Bal K Joshi ◽  
Hari P Bimb ◽  
Gopal Parajuli ◽  
Bedanand Chaudhary
Keyword(s):  
Resistance Gene ◽  
Ssr Markers ◽  
Bulked Segregant Analysis ◽  
Blast Resistance ◽  
Hot Spot ◽  
Resistance Breeding ◽  
Recurrent Parent ◽  
Simple Method ◽  
Multiple Alleles ◽  
Rice Landraces

Molecular markers tightly linked to target gene have been identified in different chromosomes to impose the genetic selection. This paper summarizes the progress and achievement made in breeding for blast resistance rice based on DNA markers. At least 40 genes conferring resistance to blast isolates with multiple alleles have been described. Both dominant and recessive resistance alleles have been found in many rice landraces. Highly polymorphic and easily detectable SSR markers are being used in breeding for blast resistance. Bulked segregant analysis (BSA) is the simple method for tagging resistance gene by SSR markers. Quantitative trait loci (QTLs) have also been mapped and most of them are linked to qualitative genes. Simple sequence repeat (SSR) markers linked to the gene are being used to select plants possessing the desired trait and markers throughout the genome are being used to select plants that are genetically similar to recurrent parent. Using SSR markers it may be possible to select blast resistance genotypes at any stage of crop development from any small part of crop, to conduct many round of selection, to select without inoculums, without scoring, and without testing in hot spot or artificial inoculation. Molecular based blast resistance breeding work is necessary to initiate in Nepal focusing on resistance gene tagging in Nepalese rice landraces and utilization.

scholarly journals Identification of novel alleles of the rice blast resistance gene Pi54

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Kumar Vasudevan ◽  
Wilhelm Gruissem ◽  
Navreet K. Bhullar
Keyword(s):  
Resistance Gene ◽  
Rice Blast ◽  
Blast Resistance ◽  
Phosphorylation Site ◽  
Allelic Diversity ◽  
Resistance Breeding ◽  
Reference Sequence ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene ◽  
Base Pair Deletion

Abstract Rice blast is one of the most devastating rice diseases and continuous resistance breeding is required to control the disease. The rice blast resistance gene Pi54 initially identified in an Indian cultivar confers broad-spectrum resistance in India. We explored the allelic diversity of the Pi54 gene among 885 Indian rice genotypes that were found resistant in our screening against field mixture of naturally existing M. oryzae strains as well as against five unique strains. These genotypes are also annotated as rice blast resistant in the International Rice Genebank database. Sequence-based allele mining was used to amplify and clone the Pi54 allelic variants. Nine new alleles of Pi54 were identified based on the nucleotide sequence comparison to the Pi54 reference sequence as well as to already known Pi54 alleles. DNA sequence analysis of the newly identified Pi54 alleles revealed several single polymorphic sites, three double deletions and an eight base pair deletion. A SNP-rich region was found between a tyrosine kinase phosphorylation site and the nucleotide binding site (NBS) domain. Together, the newly identified Pi54 alleles expand the allelic series and are candidates for rice blast resistance breeding programs.

Genome wide analysis of recurrent parent genome recovery in different backcross populations for blast resistance through SSR markers in rice

2019 ◽  
Vol 51 (6) ◽  
Author(s):  
Tanweer Fatah Abro ◽  
Mohd Rafiiyusop ◽  
Zahoor Ahmed Soomro ◽  
Abdul Wahid Baloch ◽  
Piar Ali Shar ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Blast Resistance ◽  
Recurrent Parent ◽  
Recurrent Parent Genome ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Backcross Populations

Identification of SSR markers for a broad-spectrum blast resistance gene Pi20(t) for marker-assisted breeding

2008 ◽  
Vol 22 (1) ◽  
pp. 141-149 ◽  
Author(s):  
Wei Li ◽  
Cailin Lei ◽  
Zhijun Cheng ◽  
Yulin Jia ◽  
Dongyi Huang ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Ssr Markers ◽  
Broad Spectrum ◽  
Blast Resistance ◽  
Marker Assisted Breeding ◽  
Blast Resistance Gene

scholarly journals Identification and Characterization of a New Blast Resistance Gene Located on Rice Chromosome 1 Through Linkage and Differential Analyses

2004 ◽  
Vol 94 (5) ◽  
pp. 515-519 ◽  
Author(s):  
Menglan Zhu ◽  
Ling Wang ◽  
Qinghua Pan
Keyword(s):  
Microsatellite Markers ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Bulked Segregant Analysis ◽  
Blast Resistance ◽  
Rice Chromosome ◽  
Chromosome 1 ◽  
Major Resistance Genes ◽  
High Level

The Chinese native cv. Q14 expresses a high level of resistance to many isolates of Pyricularia grisea collected from Japan, Thailand, and China. Q14 was crossed to an indica-susceptible cultivar, Q61. To rapidly determine the chromosomal location of the major resistance gene present in the cultivar, a linkage analysis using microsatellite markers was performed in the F2 population segregating 3R:1S (resistant/susceptible) through bulked-segregant analysis (BSA) in combination with recessiveclass analysis (RCA). A total of 189 microsatellite markers selected from each chromosome equally (with ≈10 centimorgans) were tested with the BSA approach. Only two markers, RM151 and RM259, located on chromosome 1 showed positive and negative polymorphisms, respectively, for a resistance gene segregating in the population. To confirm the polymorphic markers, a total of 155 viable susceptible individuals were tested with the RCA approach. The markers RM151 and RM259 were found to link to the resistance gene with recombination frequencies of 11.9 ± 2.8% and 9.7 ± 8.0%, respectively. For further characterization of the resistance gene, 3 resistance genes mapped on chromosome 1, as well as 15 major resistance genes that might be employed in the breeding program, were selected for differential tests with 85 Chinese isolates. The resistance gene identified in this research conveys reactions distinct from those conditioned by the 18 resistance genes. This new resistance gene tentatively was designated Pi27(t).

scholarly journals Identification of rapd marker linked to blast resistance gene in a somaclone of rice cultivar Araguaia

2002 ◽  
Vol 27 (2) ◽  
pp. 181-185 ◽  
Author(s):  
LEILA G. ARAÚJO ◽  
ANNE S. PRABHU ◽  
MARTA C. FILIPPI
Keyword(s):  
Oryza Sativa ◽  
Resistance Gene ◽  
Bulked Segregant Analysis ◽  
Blast Resistance ◽  
Rapd Marker ◽  
Rice Cultivar ◽  
Pyricularia Grisea ◽  
Blast Resistance Gene ◽  
F2 Population ◽  
Susceptible Bulk

The gene Pi-ar confers resistance to Pyricularia grisea race IB-45 in a somaclone derived from immature panicles of the susceptible rice (Oryza sativa) cultivar Araguaia. RAPD technique was used to identify molecular markers linked to this gene utilizing bulked segregant analysis. Initially, the two parental DNAs from the resistant donor SC09 and 'Araguaia' were analyzed using random primers. Of the 240 primers tested, 203 produced amplification products. The two parental DNAs along with the resistant and susceptible bulks of F2 population were screened using 48 primers that differentiated resistant and susceptible parents. Even though eight primers differentiated the resistant bulk from the susceptible bulk, as well as somaclone SC09 and 'Araguaia', only one primer, OPC02 ('GTGAGGCGTC'), was found to be tightly linked (1.7cM) to the resistance gene of somaclone SC09.

Molecular analysis of five rice blast resistant genes in landraces from Myanmar and Laos

2020 ◽  
Vol 18 (4) ◽  
pp. 287-293
Author(s):  
Jiqiong Ma ◽  
Yiding Sun ◽  
Yi Yang ◽  
Cuifeng Tang ◽  
Xinxiang A ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Resistance Gene ◽  
Rice Blast ◽  
Blast Resistance ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene ◽  
Rice Landraces ◽  
Resistant Genes ◽  
Functional Molecular Markers ◽  
Susceptibility Patterns

AbstractIn order to understand rice blast resistance gene patterns in rice landraces from Myanmar and Laos, we analysed the Pita, Pib, Pikh, Pi9 and Pi5 genes in 80 landraces through the use of functional molecular markers and functional fragment sequencing. These landraces were separated into four haplotypes (H1–H4) based upon three mutations identified in the Pita sequence. All four haplotypes were detected among landraces from Myanmar, whereas only the H1 and H2 haplotypes were detected among landraces from Laos. We additionally grouped landraces carrying 0–4 resistance genes into 18 genotypes based upon their resistance-susceptibility patterns and found that 16 of these genotypes were detected among Myanmar landraces at relative frequencies ranging from 2.50–12.50%, while 10 were detected among landraces from Laos at relative frequencies of 2.50–42.50%. The ‘Pib( + ) pikh(−) pi9(−) pi5(−) pita(−)’ genotype was found to be dominant, accounting for 12.5 and 42.5% of landraces from Myanmar and Laos, respectively. The Pib, Pikh, Pi5 and Pita genes were detected in 52.50, 32.50, 37.50 and 30.00% of landraces from Myanmar respectively, whereas they were detected in 52.50, 12.50, 30.00 and 17.50% of landraces from Laos, respectively. The Pi9 gene was rarely detected among analysed landraces and was particularly rare in those from Laos. These findings suggest that rice landraces from Myanmar are more genetically diverse than those from Laos, with clear differences in blast resistance gene patterns between landraces from these two countries.

scholarly journals A novel bacterial blight resistance gene from Oryza nivara mapped to 38 kb region on chromosome 4L and transferred to Oryza sativa L.

2008 ◽  
Vol 90 (5) ◽  
pp. 397-407 ◽  
Author(s):  
KULJIT K. CHEEMA ◽  
NAVJIT K. GREWAL ◽  
YOGESH VIKAL ◽  
RAJIV SHARMA ◽  
JAGJEET S. LORE ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Resistance Gene ◽  
Ssr Markers ◽  
Resistance Genes ◽  
Bacterial Blight ◽  
Oryza Sativa L ◽  
Bulked Segregant Analysis ◽  
Durable Resistance ◽  
Dominant Gene ◽  
Oryza Nivara

SummaryBacterial blight (BB) of rice caused by Xanthomonas oryzae pv oryzae (Xoo) is one of the major constraints to productivity in South-East Asia. The strategy of using major genes, singly or in combination, continues to be the most effective approach for BB management. Currently, more than two dozen genes have been designated but not all the known genes are effective against all the prevalent pathotypes. The challenge, therefore, is to continue to expand the gene pool of effective and potentially durable resistance genes. Wild species constitute an important reservoir of the resistance genes including BB. An accession of Oryza nivara (IRGC 81825) was found to be resistant to all the seven Xoo pathotypes prevalent in northern states of India. Inheritance and mapping of resistance in O. nivara was studied by using F2, BC2F2, BC3F1 and BC3F2 progenies of the cross involving Oryza sativa cv PR114 and the O. nivara acc. 81825 using the most virulent Xoo pathotype. Genetic analysis of the segregating progenies revealed that the BB resistance in O. nivara was conditioned by a single dominant gene. Bulked segregant analysis (BSA) of F2 population using 191 polymorphic SSR markers identified a ∼35 centiMorgans (cM) chromosomal region on 4L, bracketed by RM317 and RM562, to be associated with BB resistance. Screening of BC3F1 and BC2F2 progenies and their genotyping with more than 30 polymorphic SSR markers in the region, covering Bacterial artificial chromosome (BAC) clone OSJNBb0085C12, led to mapping of the resistance gene between the STS markers based on annotated genes LOC_Os04g53060 and LOC_Os04g53120, which is ∼38·4 kb. Since none of the known Xa genes, which are mapped on chromosome 4L, are effective against the Xoo pathotypes tested, the BB resistance gene identified and transferred from O. nivara is novel and is tentatively designated as Xa30(t). Homozygous resistant BC3F3 progenies with smallest introgression region have been identified.

Molecular Mapping of a Gene Conferring Fusarium Wilt Resistance in Lentil (Lens culinaris Medikus subsp. culinaris) using Bulked-segregant Analysis

2021 ◽  
Author(s):  
Jitendra Kumar Meena ◽  
H.K. Dikshit ◽  
M. Aski ◽  
Soma Gupta ◽  
Akanksha Singh ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Fusarium Wilt ◽  
Lens Culinaris ◽  
Molecular Mapping ◽  
Bulked Segregant Analysis ◽  
Resistance Breeding ◽  
Wilt Resistance ◽  
Serious Disease ◽  
Ssrs Markers ◽  
Fusarium Wilt Resistance

Background: Vascular wilt caused by Fusarium oxysporum f.sp. lentis Vasu. and Srini. is a serious disease of lentil (Lens culinaris Medikus), causes severe yield losses worldwide. For effective disease resistance breeding the inheritance and mapping of wilt resistance gene (s) is necessary. Therefore, the present investigation was focused on study the mode of inheritance and tag/map gene (s) for fusarium wilt resistance in lentil. Methods: Bulked segregant analysis (BSA) approach was used to identify markers that were tightly linked to Fusarium wilt resistance gene. The inheritance and mapping of wilt-resistance gene (s) in lentil was investigated in F2 and F2:3 populations derived from L9-12×ILL10965 cross, whereas L9-12 and ILL10965 were susceptible and resistant parents, respectively. Result: More than two hundreds SSRs markers were surveyed for the parental polymorphism, of which twenty nine were found polymorphic. These polymorphic SSRs were used for the bulked-segregant analysis (BSA) using both parents and its respective resistant and susceptible bulks, and three SSRs viz. PBALC233, PBALC1409 and PBALC203 could distinguish the respective bulks. Linkage analysis showed two SSR markers, PBALC203 and PBALC1409 flanking the wilt resistance gene at 8.2 cM and 9.4 cM distance, respectively. Further, PBLAC233 was also found present on the same linkage group at a distance of 10.2 cM from PBLAC1409.

Sign in / Sign up

Export Citation Format

Share Document