scholarly journals In Vitro Callus Induction and Regeneration Potentiality of Aromatic Rice (Oryza sativa L.) Cultivars in Differential Growth Regulators

2014 ◽  
Vol 2 (2) ◽  
pp. 160-167 ◽  
Author(s):  
Zahida Yesmin Roly ◽  
Md. Mahmudul Islam ◽  
Md. Pallob Ebna Shaekh ◽  
Md. Saiful Islam Arman ◽  
Shah Md. Shahik ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Callus Induction ◽  
Oryza Sativa L ◽  
Immature Embryo ◽  
Aromatic Rice ◽  
Rice Cultivars ◽  
Differential Growth ◽  
Soil Constraints ◽  
Important Trait

Abstract Aromatic rice (Oryza sativa L.) cultivars are strong aromatic rice cultivars which can thrive well in rice fields prone to flood, drought and other soil constraints. The present investigation was undertaken to determine a suitable media compositions for callus induction and regeneration using immature embryo of six aromatic grown rice cultivars of Bangladesh, namely, Chinigura, Kalijira, Radhuni Pagal, Modhumala, Kataribog and Mohonbhog. For callus induction different concentrations and combinations of 2, 4-Dichlorophenoxyaceticacid (2, 4-D) along with NAA were evaluated. Maximum callus induction (97.22%) was observed in Kalijira when 2 mg/L of 2, 4-D and 0.5 mg/l NAA was used and less Modhumala (66.67%) and remaining cultivars showed moderate. For regeneration initially different concentrations and combinations of 6-BenzylAminoPurine (BAP) and Indole-3-Acetic Acid (IBA) were tested. Maximum regeneration frequency (91.67%) was observed Kalijira when the optimum concentrations and combinations of 0.5 mg/l of BAP + 0.1 of mg/l IBA were used. Presently optimized regeneration method holds promise for facilitating the deployment of agronomical important trait through genetic transformation for the improvement of this important food crops.DOI: http://dx.doi.org/10.3126/ijasbt.v2i2.10313Int J Appl Sci Biotechnol, Vol. 2(2): 160-167 

scholarly journals Effects of Cultivation Media on In vitro Callus Induction and Regeneration Capabilities of Pakaumpuel Rice (Oryza sativa L.), Thai Rice Landrace

2018 ◽  
Vol 17 (1) ◽  
pp. 37-46
Author(s):  
Attachai TRUNJARUEN ◽  
Sayam RASO ◽  
Pitakpong MANEERATTANARUNGROJ ◽  
Worasitikulya TARATIMA
Keyword(s):  
Oryza Sativa ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Oryza Sativa L ◽  
Light Condition ◽  
Ms Medium ◽  
Rice Landraces ◽  
Rice Landrace ◽  
Number Of Leaves

Current study determined the effects of different cultivation media and some factors on in vitro callus induction and shoot regeneration of Pakaumpuel rice (Oryza sativa L.), the Thai rice landrace. Pakaumpuel’s dehusked seeds were surface sterilized by 20% Sodium hypoclorite for 20 mins before washed and cultured on Murashige and Skoog (MS) medium in various concentrations of BAP (0, 0.1, 0.5 and 1 mg/l) and 2,4-D (0, 1, 2 and 3 mg/l) and cultivated  in off -light or dark cycle condition for 15 days. Results showed that MS medium with 2 mg/l 2,4-D incubated in light condition provided the highest callus induction percentage (80 %). For shoot regeneration, calli were desiccated by using silica gel for 0, 30, 90 and 180 minutes prior to culture on MS medium with 1 mg/l NAA and various concentrations of BAP (0, 1, 2, 3 and 4 mg/l) for 30 days. The results revealed that desiccation periods had no effect on regeneration percentage and number of shoot per callus, but had effects on shoot length and number of leaves per shoot. Therefore, MS medium with 1 mg/l NAA and 3 mg/l BAP induced the highest regeneration percentage (53.33 %) and number of shoot per callus (4.40 shoots), while the longest shoot (12.82 cm) and the highest number of leaves per shoot (3.00 leaves) were found on the culture on this medium after desiccation for 90 minutes. This discovery can be applied for micropropagation of other Thai rice landraces.

Development of superior somaclones of aromatic local cultivar of rice (Oryza sativa L.)

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Bidhan Roy
Keyword(s):  
Tissue Culture ◽  
Oryza Sativa ◽  
Genetic Variability ◽  
Oryza Sativa L ◽  
Maximum Yield ◽  
Aromatic Rice ◽  
Rice Cultivars ◽  
Yield Increase ◽  
Local Cultivar ◽  
Parental Cultivar

The traditional local aromatic rice cultivars are poor combiners. Here we report development of superior somaclones plants through tissue culture. The somaclones, TC 4/8 had the maximum yield followed by TC 5-1, TC 4/4, TC 4/5, and TC 4/7. Yield increase of TC 4/8, TC-5-1 was 54.75% and 50.33% more over the yield of parental cultivar, respectively. The plant tissue culture thus may be recommended to create genetic variability in rice as a trustworthy biotechnological tool.

Callus Induction and in vitro Plant Regeneration of Rice (Oryza sativa L.) Under Various Conditions

2008 ◽  
Vol 11 (2) ◽  
pp. 255-259 ◽  
Author(s):  
Muhammad Tariq ◽  
Gowher Ali ◽  
Fazal Hadi ◽  
Shakeel Ahmad ◽  
Nasir Ali ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Plant Regeneration ◽  
Callus Induction ◽  
Oryza Sativa L ◽  
In Vitro Plant Regeneration ◽  
In Vitro Plant
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