scholarly journals Callus Induction and Plantlet Regeneration Ability of Selected Sri Lankan Traditional Rice Cultivars (Oryza Sativa L.) in Caryopses Culture

2013 ◽  
Vol 15 (2) ◽  
pp. 15
Author(s):  
N Dahanayake ◽  
AL Ranawake
2010 ◽  
pp. 44-49
Author(s):  
MY Miah ◽  
ME Hoque ◽  
MM Islam ◽  
M Ahmed ◽  
Kazi Noor-E-Alam Jewel

Callus induction and plant regeneration ability of the dehusked rice genotypes are important tools for varietal improvement. This investigation presents the comparative callus induction and plant regeneration as obtained from the dehusked seeds of rice cultured on MS (Murashige-Skoog) medium. In the experiment conducted in a Complete Randomized Design under laboratory conditions, dehusked seeds of Ranisalute, Chiniatap, Somudrafena, Chiniguri-2 and Katchra rice cultivars grown in South West Bangladesh were tested on autoclaved and agarified MS nutrient medium with four replications to study their callus induction and subsequent plant regeneration ability. Chiniguri-2 yielded the highest calli and plant regeneration.


2014 ◽  
Vol 2 (2) ◽  
pp. 160-167 ◽  
Author(s):  
Zahida Yesmin Roly ◽  
Md. Mahmudul Islam ◽  
Md. Pallob Ebna Shaekh ◽  
Md. Saiful Islam Arman ◽  
Shah Md. Shahik ◽  
...  

Abstract Aromatic rice (Oryza sativa L.) cultivars are strong aromatic rice cultivars which can thrive well in rice fields prone to flood, drought and other soil constraints. The present investigation was undertaken to determine a suitable media compositions for callus induction and regeneration using immature embryo of six aromatic grown rice cultivars of Bangladesh, namely, Chinigura, Kalijira, Radhuni Pagal, Modhumala, Kataribog and Mohonbhog. For callus induction different concentrations and combinations of 2, 4-Dichlorophenoxyaceticacid (2, 4-D) along with NAA were evaluated. Maximum callus induction (97.22%) was observed in Kalijira when 2 mg/L of 2, 4-D and 0.5 mg/l NAA was used and less Modhumala (66.67%) and remaining cultivars showed moderate. For regeneration initially different concentrations and combinations of 6-BenzylAminoPurine (BAP) and Indole-3-Acetic Acid (IBA) were tested. Maximum regeneration frequency (91.67%) was observed Kalijira when the optimum concentrations and combinations of 0.5 mg/l of BAP + 0.1 of mg/l IBA were used. Presently optimized regeneration method holds promise for facilitating the deployment of agronomical important trait through genetic transformation for the improvement of this important food crops.DOI: http://dx.doi.org/10.3126/ijasbt.v2i2.10313Int J Appl Sci Biotechnol, Vol. 2(2): 160-167 


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