scholarly journals Genetic Diversity Assessment of Acid Lime (Citrus Aurantifolia Swingle) Landraces of Eastern Nepal Using RAPD Markers

2014 ◽  
Vol 2 (3) ◽  
pp. 315-327 ◽  
Author(s):  
NN Munankarmi ◽  
RL Shrestha ◽  
N Rana ◽  
JKC Shrestha ◽  
S Shrestha ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Citrus Aurantifolia ◽  
Ecological Zones ◽  
Diversity Assessment ◽  
Acid Lime ◽  
Agro Ecological Zones

Acid lime (Citrus aurantifolia Swingle) is an important commercial fruit crop, cultivated from terai to high hill landscapes of Nepal. However, production and productivity is very low due to various reasons including infestations by various diseases and pests, lack of diseases and pests resistant and high yielding varieties. In this context, determination of genetic variation at molecular level is fundamental to citrus breeders for the development of elite cultivars with desirable traits. In the present study, Random Amplified Polymorphic DNA (RAPD) marker technique has been employed to assess genetic diversity in 60 acid lime landraces representing different agro-ecological zones of eastern Nepal. Nine selected arbitrary primers generated 79 RAPD fragments of which 75 were polymorphic (94.94%). Phenogram was constructed by NTSYSPC ver. 2.21i using UPGMA cluster analysis based on Jaccard’s similarity coefficient to deduce overall genetic diversity and relationships of the acidlime genotypes under study. Sixty acid lime landraces formed seven clusters and similarity value ranged from 38% to 98% with an average of 72%. Genetic variation at different agro-ecological zones was assessed using Popgene ver. 1.32 and found 47% to 69.6% polymorphism. Shannon’s index and Nei’s gene diversity showed highest level of acid lime diversity in Terai zone (PPB, 69.62%; H, 0.213; I, 0.325) followed by mid-hill zone (PPB, 67.09%; H, 0.208; I, 0.317). The results obtained will be useful to citrus breeders for elite cultivar development. The RAPD-PCR technique is found to be the rapid and effective tool for genetic diversity assessment in acid lime landraces of Nepal. DOI: http://dx.doi.org/10.3126/ijasbt.v2i3.10950  Int J Appl Sci Biotechnol, Vol. 2(3): 315-327  

scholarly journals Genetic Diversity Assessment of Acid Lime (<i>Citrus aurantifolia</i> Swingle) Landraces in Nepal, Using SSR Markers

2012 ◽  
Vol 03 (12) ◽  
pp. 1674-1681 ◽  
Author(s):  
Ram Lal Shrestha ◽  
Durga Datta Dhakal ◽  
Durga Mani Gautum ◽  
Krishna Prasad Paudyal ◽  
Sangita Shrestha
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Citrus Aurantifolia ◽  
Diversity Assessment ◽  
Acid Lime

scholarly journals Genetic diversity assessment of neem (Azadirachta indica A. Juss) in northern Nigeria

2021 ◽  
Author(s):  
Barka Peter MSHELMBULA ◽  
Geoffrey Obinna Anoliefo ◽  
Beckley Ikhajiagbe ◽  
Boniface Edegbai
Keyword(s):  
Genetic Diversity ◽  
Germplasm Conservation ◽  
Western Hemisphere ◽  
Northern Nigeria ◽  
Important Species ◽  
Tropical Regions ◽  
Ecological Zones ◽  
Diversity Assessment ◽  
Wide Range ◽  
Agro Ecological Zones

Neem is a tropical tree that can adapt to a wide range of places and particularly to semi- arid conditions. As at present, it is grown in many Asian countries and also in the tropical regions of the western hemisphere. Genetic variability and diversity are a major requirement needed for both immediate results and the one ones thereafter adaptation of plant types in their original domain. The evaluation of genetic diversity of any species is extremely crucial for their sustainability, continuity, survival and gene manipulation. Major breakthroughs in the field of molecular biology was able to develop several tools for the investigation of genetic diversity at the genome level to determine phylogenetic relationships among inter or intra-species. The advent of molecular markers for the detection and exploitation of DNA polymorphism is one of the major breakthroughs in the world of molecular genetics. The importance of genetic diversity in plant germplasm conservation, especially in economically important species such as Azadirachtaindica, is enormous, particularly in Nigeria. The question is whether A. indica from different Agro-ecological zones have genetic variations or similarities. This was the bane of the current study, which used RAPD to look atgenetic diversity of 27 randomly selected neem trees within the agro-ecological zones in Northern Nigeria. A total of 9 primers were employed out of which only 5 were responsive (OPA-02, OPA-03, OPA-15 and OPA-19). These primers showed dissimilarities in the visible DNA bands among the various tree samples. There was evidence of genetic dissimilarities among the trees sampled. Differences in percentage polymorphism was reported, where it was reportedly highest among the Borno State tree samples (97.44%), compared to those in Yobe State with no polymorphism.

scholarly journals ISSR-Markers Assisted Genetic Diversity Assessment of Acid Lime [Citrus aurantifolia (Christm.) Swingle] Germplasm of Eastern Nepal

2018 ◽  
Author(s):  
Nabin Narayan Munankarmi ◽  
Neesha Rana ◽  
Tribikram Bhattarai ◽  
Ram Lal Shrestha ◽  
Bal Krishna Joshi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Binary Data ◽  
Issr Markers ◽  
Data Matrix ◽  
Dice Similarity Coefficient ◽  
Computer Algorithms ◽  
Significant Information ◽  
Citrus Aurantifolia ◽  
Diversity Assessment ◽  
Acid Lime

Acid lime [Citrus aurantifolia (Christm.) Swingle] is a fruit crop, enriched with high commercial value and is cultivated in 60 out of 75 districts representing all geographical landscapes of Nepal. Lack of high yielding cultivars is probably one of the main reason for its extremely reduced productivity which warrants a deep understanding of genetic diversity in existing germplasm. Hereby, we aim to access the genetic diversity of acid lime germplasm cultivated at 3-different ecological gradients of eastern Nepal employing PCR-based Inter-Simple Sequence Repeats markers (ISSR). Altogether, 21 polymorphic ISSR markers were used to assess the genetic diversity in 60 acid lime cultivars sampled from different geographical locations. Analysis of binary data matrix was performed on the basis of bands obtained, scoring of the data was done accordingly, and principal coordinate analysis and phenogram were constructed using different computer algorithms. ISSR profiling yielded 234 amplicons, of which 87.18% were found to be polymorphic. The number of amplified fragments ranged from 7-18 with amplicon size ranging from 250-3200 bp. The NTSYS based Cluster analysis using UPGMA algorithm taking Dice Similarity coefficient separated 60 accessions into 2-major and 3-minor clusters. The genetic diversity analysis revealed the highest for Terai and the lowest for High-hill zone. Cluster I comprised of accessions from High-hill and Mid-hill regions revealing the close genetic relationship, whereas cluster II comprised of accessions from all three agro-ecological zones and the exotic varieties. Furthermore, our results revealed the accessions harvested from different geographical gradients were not genetically distinct, but highest diversity was observed in Terai accessions in comparison to the regions belonging to the High and Mid-hills. Thus, our data indicate that the ISSR provides a better option for evaluating the genetic diversity of Nepalese Acid Lime cultivars and furnished significant information, assisting parental selection in current and future breeding programs and germplasm conservation which ultimately may help to provide a technological breakthrough for the farmers of the developing country like Nepal.

scholarly journals Genetic Analysis of Pinus sylvestris L. and Pinus sylvestris forma turfosa L. Using RAPD Markers

2010 ◽  
Vol 2 (1) ◽  
pp. 129-132 ◽  
Author(s):  
Beáta ÁBRAHÁM ◽  
Ildikó MIKLÓSSY ◽  
Erika KOVÁCS ◽  
Éva TAMÁS ◽  
Ildikó MÉSZÁROS ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Pinus Sylvestris ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Raised Bogs ◽  
Low Genetic Diversity ◽  
Genetic Differentiation Of Populations ◽  
Fossil Records ◽  
Continental Glaciation

The purpose of the present study was to determine the level of genetic diversity within and among Ciuc basin, Romania (populations from Mohos and Luci raised bogs in Harghita Mountain and Sumuleu in Ciuc Mountain) Pinus sylvestris populations using molecular markers. Two of populations (Mohos and Luci) seems to be the descendants that survived the continental glaciation. Genetic diversity was analyzed by RAPD (Random Amplified Polymorphic DNA). Nine primers were selected for analysis, which generated reproducible bands. On base of presence or absence of homologues bands Nei’s gene diversity, the percentage of polymorphic loci and Nei’s unbiased genetic distance were calculated. The level of genetic variation among populations was found to be low. For both populations the variation values among populations were higher than within populations. The fossil records and geological historical data explain the extremely low genetic diversity of this species. Pinus sylvestris experienced strong bottlenecks during its evolutionary history, which caused the loss of genetic variation. Genetic drift and breeding in post-bottlenecked small populations may be the major forces that contribute to low genetic diversity and genetic differentiation of populations. Human activities may have accelerated the loss of genetic diversity in Pinus sylvestris.

Genetic Diversity of Aphid (Hemiptera: Aphididae) Species Attacking Amaranth and Nightshades in Different Agro-Ecological Zones of Kenya and Tanzania

2018 ◽  
Vol 26 (2) ◽  
pp. 407-421
Author(s):  
A.C. Kipnyargis ◽  
F.M. Khamis ◽  
E.U. Kenya ◽  
S. Ekesi ◽  
K.K.M. Fiaboe
Keyword(s):  
Genetic Diversity ◽  
Ecological Zones ◽  
Agro Ecological Zones

scholarly journals RAPD ANALYSIS OF GENETIC VARIATION IN NATURAL POPULATIONS OF AEGILOPS SP. FROM SOUTH ADRIATIC

AGROFOR ◽  
2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Miodrag DIMITRIJEVIĆ ◽  
Sofija PETROVIĆ ◽  
Borislav BANJAC ◽  
Goran BARAĆ
Keyword(s):  
Genetic Variation ◽  
Food Production ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Gene Diversity ◽  
Rapd Marker ◽  
Natural Populations ◽  
Genetic Structuring ◽  
Potential Source ◽  
Novel Approach

New challenges that food production is facing, requires novel approach inagricultural strategy. The scissors of growing demand for food and the limits of theEarth's resources are forcing plant breeders to run for the new borders, utilizing allthe available genetic variation in order to create fruitful and economically soundcultivars. Aegilops sp. (Poaceae) is a potential source of genetic variation for wheatimprovement. RAPD marker analysis was used in order to distinguish and evaluatedifferent genotypes of Aegilops sp. population samples from the collectiongathered during few years’ expeditions in South Adriatic, along the coastal, littoraland the inland parts of Montenegro. Ten randomly amplified polymorphic DNAmarkers (RAPDs) were tested: OPA-05, OPA-08, OPB-06, OPA-02, OPA-07,OPA-25, OPB-07, OPB-18, OPC-06, OPC-10 to examine genetic structuring on 18samples of 6 populations of different Aegilops sp. According to global AMOVA,75% of total gene diversity was attributable mostly to diversity within population(ΦPT =0.205 p=0.001), indicating that the groups of studied goat grass populationswere seemingly to differing genetically. In contrast, 25% of the variation camefrom variation among populations. According to PCoA, the distribution of 18 goatgrass accessions by Principal Coordinate Analysis shows 3 distinct groups. PCoaxis 1, PCo axis 2, and PCo axis 3 account for 20.8%, 18.2% and 14.1% of thevariation, respectively. The results showed that RAPD markers could be aconvenient tool for investigating genetic variation and for detecting geneticstructuring of populations. Genetic variability formed under natural selection wasentrenched.

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