scholarly journals Isolation of Ethidium Bromide Degrading Bacteria from Jharkhand

2017 ◽  
Vol 5 (3) ◽  
pp. 293-301 ◽  
Author(s):  
Anil Kumar ◽  
Preeti Swarupa ◽  
Vikram Pal Gandhi ◽  
Snehal Kumari
Keyword(s):  
Microbial Community ◽  
Ethidium Bromide ◽  
Gel Electrophoresis ◽  
Uv Light ◽  
Liquid Waste ◽  
Agarose Gel ◽  
Degrading Bacteria ◽  
Zone Characteristic ◽  
Waste Soil ◽  
Halo Zone

Ethidium bromide (EtBr) is a carcinogenic and mutagenic agent which is widely used in research laboratories to probe nucleic acids by gel electrophoresis. It is generally buried underground (for solid waste) or disposed of pouring it down the sink (in case of liquid waste). Soil or drain microbial community may be able to take care of such substance else it will lead to contamination of our underground resources or others through defined and undefined routes. In view of the above assumption and literature reports the present study was undertaken to isolate and evaluate bacteria for removal, by bioaccumulation and /or biotransformation, of EtBr from contaminated sources and wastes, before their disposal to the environment. Two distinct bacteria both motile BR3 and BR4 could be identified from agarose-gel-waste containing 0.5-1.0 µg/ml ethidium bromide. Both bacteria were found to grow on EtBr-NA plate (Nutrient-Agar supplemented with EtBr at a concentration of 30 µg/ml) however only BR3 isolate showed large non-fluorescent-halo zone (characteristic to degradation of EtBr) when exposed to trans-UV light. Other isolate BR4 could accumulate EtBr within the colony biomass but did not showed clear (non-fluorescent) hallow zone around it. However the bacterium is not able to utilize the EtBr as a sole carbon source.Int. J. Appl. Sci. Biotechnol. Vol 5(3): 293-301

Comparative analysis of the DNA staining efficiencies of different fluorescent dyes in preparative agarose gel electrophoresis

2005 ◽  
Vol 43 (8) ◽  
Author(s):  
Qing Huang ◽  
Wei-Ling Fu
Keyword(s):  
Comparative Analysis ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Ethidium Bromide ◽  
Gel Electrophoresis ◽  
Fluorescent Dyes ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Dna Staining

AbstractEthidium bromide (EB) is a mutagen and toxin that is widely used in the laboratory for visualization of nucleic acids. Safer nucleic acid stains, such as SYBR

Genotyping microsatellite polymorphisms by agarose gel electrophoresis with ethidium bromide staining

1998 ◽  
Vol 8 (4) ◽  
pp. 227-233 ◽  
Author(s):  
T. N. Ferraro ◽  
J. F. Schill ◽  
C. Ballas ◽  
N. Mulholland ◽  
G. T. Golden ◽  
...  
Keyword(s):  
Ethidium Bromide ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Ethidium Bromide Staining

Comparison of ethidium bromide-stained agarose gel electrophoresis and automated fragment analysis for evaluation of IgH gene products

2001 ◽  
Vol 25 (9) ◽  
pp. 769-774 ◽  
Author(s):  
Beate Gleissner ◽  
Juergen Maurer ◽  
Annette Sindram ◽  
Richard Reinhard ◽  
Eckhard Thiel
Keyword(s):  
Ethidium Bromide ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Gene Products ◽  
Agarose Gel Electrophoresis ◽  
Fragment Analysis

scholarly journals A comparison of DNA stains and staining methods for Agarose Gel Electrophoresis

2019 ◽  
Author(s):  
Andie C. Hall
Keyword(s):  
Nucleic Acid ◽  
Ethidium Bromide ◽  
Gel Electrophoresis ◽  
Careful Consideration ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Cost Increase ◽  
Dna Migration ◽  
Discernible Effect ◽  
Staining Methods

ABSTRACTNucleic acid stains are necessary for Agarose Gel Electrophoresis (AGE). The commonly used but mutagenic Ethidium Bromide is being usurped by a range of safer but more expensive alternatives. These safe stains vary in cost, sensitivity and the impedance of DNA as it migrates through the gel. Modified protocols developed to reduce cost increase this variability. In this study, five Gel stains (GelRed™, GelGreen™, SYBR™ safe, SafeView and EZ-Vision®In-Gel Solution) two premixed loading dyes (SafeWhite, EZ-Vision®One) and four methods (pre-loading at 100x, pre-loading at 10x, precasting and post-staining) are evaluated for sensitivity and effect on DNA migration. GelRed™ was found to be the most sensitive while the EZ-Vision® dyes and SafeWhite had no discernible effect on DNA migration. Homemade loading dyes were as effective as readymade ones at less than 4% of the price. This method used less than 1% of the dye needed for the manufacturer recommended protocols. Thus, with careful consideration of stain and method, Gel stain expenditure can be reduced by over 99%.

Luminography – an Alternative Assay for Detection of von Willebrand Factor Multimers

1988 ◽  
Vol 60 (02) ◽  
pp. 133-136 ◽  
Author(s):  
R Schneppenheim ◽  
H Plendl ◽  
U Budde
Keyword(s):  
Von Willebrand Factor ◽  
Gel Electrophoresis ◽  
Detection Methods ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Luminescence Assay ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryA luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

Identification of Fibrinogen Derivatives in Plasma Samples

1972 ◽  
Vol 27 (03) ◽  
pp. 610-618 ◽  
Author(s):  
H Graeff ◽  
R von Hugo
Keyword(s):  
Molecular Weight ◽  
Gel Electrophoresis ◽  
Gel Chromatography ◽  
Agarose Gel ◽  
Plasma Samples ◽  
Methodological Study ◽  
Parent Molecule ◽  
Electrophoretic Mobilities ◽  
Fibrin Monomer

SummaryThe observation of fibrinogen derivatives with a molecular weight higher than the parent molecule in human cases of DIC initiated the present methodological study. These derivatives were identified by the following methods : 2.5 M β-alanine precipitation of the plasma samples, PAA gel electrophoresis, intra gel immunoprecipitation and agarose gel chromatography. In the plasma of a patient with severe eclampsia and laboratory signs of DIC two derivatives with a molecular weight higher than that of fibrinogen were identified according to their relative electrophoretic mobilities: 0.18 and 0.28 × 10−5 cm2/V × sec (fibrinogen: 0.43 × 10−5 cm2/V × sec). Electrophoretic studies in the presence of 5 M urea indicated that the 0.28 derivative is a complex probably formed by fibrinogen and a fibrin monomer.

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