Selective Flamingo Medium for the Isolation of Aspergillus fumigatus

For various studies in the clinic as well as the environment, it is essential to be able to selectively isolate Aspergillus fumigatus from samples containing bacteria as well as various other fungi (mainly Mucorales). Six agar media were compared for effectiveness in selectively isolating Aspergillus fumigatus from agricultural plant waste, woodchip waste, green waste, soil, grass and air samples collected in the Netherlands at a 48 °C incubation. The Flamingo Medium incubated at 48 °C, provided the most effective condition for the isolation of A. fumigatus from environmental samples, since it effectively inhibited the growth of competing fungi (mainly Mucorales) present in the environmental samples. Flamingo Medium reduced the number of colonies of Mucorales species by 95% and recovered an average of 20−30% more A. fumigatus colonies compared to the other media. We further confirmed that Flamingo Medium can inhibit the growth of clinical Mucorales, which occasionally present in patient’s tissue and can also be used for clinical applications. We suggest the use of Flamingo Medium as an efficient method for the study of A. fumigatus from important environmental niches for which there is increasing interest. Additionally, it can also be used in the clinic to isolate A. fumigatus especially from tissue contaminated with Mucorales.

Hydraulic conductivity and calcium carbonate content of biocemented heavy-metal contaminated mine waste soil

It is not uncommon for mining activity to generate wastes associated with negative engineering impacts include susceptibility to runoff due to the absence of vegetation, erosion, and sinkhole. Due to their high degree of permeability, movement of heavy metal contaminants in waste soils and aquifers occurs actively along with the hydrogeological parameters, and research on using biocementation methods such as enzyme induced calcite precipitation (EICP) to reduce the hydraulic conductivity is therefore beneficial. Mine waste soil collected from a copper mine in Lohan Dam, Sabah is characterized physically, morphologically, and chemically then treated with EICP under different operational parameters include curing duration (1,3,7 days), curing temperatures (5, 10, and 25 oC ), and relative density (70 and 80 %). The hydraulic conductivity was then tested using a constant head permeability test and the calcium carbonate content (%) is determined using the HCL washing method. Properties of the Lohan Dam wastes are found to be predominantly coarse grain soil of low plasticity, high specific gravity, high permeability, acidic in nature, and low organic content. Morphologically, they are composed of powdered and hardened particles with dark brown color with high amount of irregular-shaped particles. Mineralogical, Lohan Dam soil wastes contain a high level of heavy metals beyond the safety level of the Department of Environmental Malaysia. EICP treatment had to change the degree of permeability from ‘high and medium’ to ‘low’ with a 94-97% reduction in hydraulic conductivity corresponding to the amount of calcium carbonate content produced ranging 6.94-9.63%. In conclusion, relative density shows the marginal effect, curing duration, and temperature shows a more significant impact on the treatment effectiveness.

STUDY OF PHYSICAL AND MECHANICAL CHARACTERISTICS OF VARIOUS TYPES OF SOIL STRENGTHENED BY CLINKER WASTE

the specificity of the construction of roads is the use of a huge amount of various stone materials - sand, gravel, etc. To reduce the cost of road pavements, it is possible to replace stone materials with local soils. Such materials do not need to be transported by road or rail. It is known that most soils cannot be used in the construction of the road bed (sandy loam and loam are light dusty and heavy dusty, clay dusty and oily), as their use leads to the appearance of various deformations of the upper layers of road pavement. This problem can be solved by strengthening the soil with various types of binders, in particular, clinker waste. When conducting experimental studies to assess the effectiveness of the use of waste clinker production to strengthen the soil the following compositions without using: soil and 9% waste; soil and 18% waste; soil and 27% waste were investigated. As a result of the research, the main dependences of the changes in the physical and mechanical parameters of the soil concrete on the amount of waste clinker production introduced into the composition of various types of soils were identified. It was found that the use of waste clinker production to strengthen the loam allowed to obtain a reinforced soil brand M20. Strengthening sand with clinker production without the addition of cement is not practical, as the samples do not gain even minimal strength for 180 days, which allows them to be classified by brand, in addition, such samples have extremely low resistance.

Strength Characteristics of Cement Treated and Expanded Polystyrene Mixed Lightweight of Waste Soil from the Construction Site of a Yangtze River Bridge in China

In the past decades, the speed and scale of Chinese infrastructure construction have been enormous, and the resulting construction waste is also quite amazing, which has become a huge threat to environmental protection. If the pollution-free engineering utilization of the construction waste can be achieved at a low cost, it will undoubtedly be a great benefit for the country and the people. Therefore, a preliminary experimental study was conducted to investigate the strength characteristics of the cement treated and expanded polystyrene mixed lightweight. The waste soil (muddy clay and fine sand) as the main component of the lightweight mixture is taken from a foundation construction site of a Yangtze River Bridge which connects two eastern coastal cities, Zhenjiang and Yangzhou. With different mixture ratios and additives of the cement treated and expanded polystyrene mixed lightweight, a series of cubic samples were tested by the unconfined compressive strength test, and collections of standard cylinder samples prepared by hand were inspected by the conventional triaxial shear test. Then, a good exponential relationship between the uniaxial compressive strength and the cement mixing ration was founded, and a logarithmic relationship was captured between the compressive strength and curing time within a period of 28 days after the specimens were made. Samples made of different soil as raw materials have different strengths, but all of these specimens show a strain-hardening and stable behavior. Results show that the mechanical strength characteristics of the cement treated and expanded polystyrene mixed waste soil mainly depend on the proportions of lightweight mixture.