scholarly journals Prevalence and Antimicrobial Susceptibility Pattern of Pseudomonas aeruginosa Isolated from Various Clinical Samples in a Tertiary Care Hospital

2020 ◽  
Vol 8 (2) ◽  
pp. 11-17
Author(s):  
Surya Narayan Mahaseth ◽  
Lokeshwar Chaurasia ◽  
Brajesh Jha ◽  
Raj Kumari Sanjana
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Susceptibility ◽  
Tertiary Care Hospital ◽  
Chemotherapeutic Agent ◽  
Tertiary Care ◽  
Clinical Samples ◽  
Care Hospital ◽  
Susceptibility Pattern ◽  
Antimicrobial Susceptibility Pattern ◽  
Susceptibility Rate

Background and Objectives: Pseudomonas aeruginosa is one of the leading causes of hospital acquired infections. Increased resistance in this organism continues to pose a significant threat to patient care because of limited therapeutic options. The main objective of this study was to find out the prevalence and current antimicrobial susceptibility pattern of P. aeruginosa isolates obtained from various clinical samples at a tertiary care hospital. Material and Methods: The study was conducted in a tertiary care hospital in Bharatpur, Chitwan, Nepal on 453 isolates of Pseudomonas aeruginosa from various clinical samples. The colonies which were grown on culture media were identified by different standard biochemical tests. Antimicrobial susceptibility testing was done using Kirby–Bauer disc diffusion method and the results were interpreted according to the CLSI guidelines. Quality control of the test was done by standards ATCC strain of P. aeruginosa 27853. Results: This present study revealed the prevalence rate of P. aeruginosa was 11.29%. Piperacillintazobactam was the most sensitive chemotherapeutic agent with 94.26% susceptibility rate, followed by imipenem 89.40% and levofloxacin 88.08%. Amikacin showed better susceptibility rate 67.33% than that of gentamicin 48.78%; the susceptibility rate to cephalosporin and aztreonam was relative very low. Most of the P. aeruginosa strains were isolated from clinical samples like sputum 206, urine 81, respiratory secretion 76, and pus 35. Out of 453 clinical isolates, 167(36.86%) clinical isolates of P. aeruginosa were found to be MDR. Conclusion: Most of the P. aeruginosa strains were isolates from sputum, urine, respiratory secretions and pus samples and were found to be MDR. Piperacillin-tazobactam was the most sensitive chemotherapeutic agent followed by Imipenem, levofloxacin.

scholarly journals Clinical Profile and Susceptibility Patterns of Infections by Elizabethkingia Species in a Tertiary Care Hospital, Bhubaneswar, Odisha

2021 ◽  
Vol 8 (21) ◽  
pp. 1624-1629
Author(s):  
Preety Mishra ◽  
Dipti Pattnaik ◽  
Kalpana Mund ◽  
Subhra Snigdha Panda ◽  
Smrutilata Sahoo ◽  
...  
Keyword(s):  
Nosocomial Infection ◽  
Antimicrobial Susceptibility ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Clinical Profile ◽  
Clinical Samples ◽  
Care Hospital ◽  
Susceptibility Pattern ◽  
Antimicrobial Susceptibility Pattern ◽  
Elizabethkingia Meningoseptica

BACKGROUND Elizabethkingia meningoseptica is an emerging pathogen causing meningitis, pneumonia, endocarditis, bacteremia, sepsis, wound & soft tissue infections, abdominal, respiratory and ocular infections, dialysis associated peritonitis and prosthesis associated septic arthritis, especially in immunodeficient hosts of various age groups. The prevalence of nosocomial infection by E. meningoseptica has increased, predominantly in patients with invasive procedures, prior use of broad-spectrum antimicrobial and co-morbid conditions. We wanted to determine the prevalence of Elizabethkingia meningoseptica among the clinical samples processed in our laboratory and their antimicrobial susceptibility pattern. METHODS This observational study was conducted in patients admitted to a tertiary care hospital, from October 2017 to October 2020. The study subjects were selected on positive bacterial culture reports after excluding patients of less than 18 years of age and their demographic and clinical features were obtained from their medical records. Blood samples were processed by BacT/Alert. VITEK-2 system was used to identify the bacteria and their antimicrobial susceptibility pattern. RESULTS Among the 3532 clinical samples processed, 16 (0.45 %) bacterial isolates were Elizabethkingia meningoseptica. Out of them, 5 (31.25 %) were from blood and 11 (68.75 %) were from endotracheal tubes. More number of cases 6 (38 %) were seen in the age group of 61 - 70 years. Most of the patients were on mechanical ventilation with common co-morbid condition associated was cardiovascular diseases 11 (68 %). E. meningoseptica was most often sensitive to nalidixic acid and ciprofloxacin (50 %), tigecycline 4 (30 %), minocycline 3 (18 %), cotrimoxazole 2 (15 %), piperacillin-tazobactam 1 (13 %) and minocyclin (18.75 %). CONCLUSIONS Infection with E. meningoseptica is clinically important as the organism causes nosocomial infection and is intrinsically resistant to multiple antibiotics, such as βlactams, aminoglycosides, tetracycline, tigecycline, colistin, chloramphenicol and carbapenems. Early diagnosis and prompt treatment is required to prevent the morbidity and mortality. KEYWORDS Elizabethkingia Meningoseptica, Antimicrobial Susceptibility, Clinical Profile

scholarly journals Isolation, Identification, Speciation and Antibiotic Susceptibility Pattern of Klebsiella Species among Various Clinical Samples at Tertiary Care Hospital

2021 ◽  
pp. 78-87
Author(s):  
K. Sathyavathy ◽  
B. Kiran Madhusudhan
Keyword(s):  
Antimicrobial Susceptibility ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Clinical Samples ◽  
Care Hospital ◽  
Susceptibility Pattern ◽  
Klebsiella Species ◽  
Antimicrobial Susceptibility Pattern ◽  
Strip Method ◽  
Phenotypic Methods

To isolate, identify and speciate Klebsiella from various clinical samples and their Antimicrobial susceptibility pattern at tertiary care hospital, Chennai. Out of this 76 ESBL producing Klebsiella pneumoniae, 64(84%) were positive by Double Disc Synergy Test (DDST), 71(93%) by phenotypic confirmatory disc diffusion test (PCDDT) and 69(90.7%) by Etest strip method. Sensitivity was found to be maximum (93%) by PCDDT method, compared to other phenotypic methods such as DDST and Etest strip method. The Present study also highlights the need for the continued monitoring of Antimicrobial susceptibility patterns of important bacterial pathogens, so that rational antibiotic policies can be formulated.

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