scholarly journals Micropropagation of Butea buteiformis (Voigt) Grierson and Long

2007 ◽  
Vol 7 ◽  
pp. 119
Author(s):  
Belai Meeta Singh ◽  
Cristoph Wawrosch ◽  
Sanu Devi Joshi ◽  
Brigitte Kopp
Keyword(s):  
Acetic Acid ◽  
Analytical Procedure ◽  
Science And Technology ◽  
Nodal Cuttings ◽  
Half Strength ◽  
Mature Seeds ◽  
Indole 3 Acetic Acid

Mature seeds of Butea buteiformis were cultured on half strength Murashige and Skoog (1962) medium. Nodal cuttings were used as explants from in vitro growing plants for experimentation. Plants were well grown on the medium supplemented with benzylaminopurine (BAP) 0.5 µM / l and indole-3-acetic acid (IAA) 1.0 µM/ l. Such propagated plants were acclimatized very well and transferred to the field. All the collected data were worked out statistically with SPSS, a system of analytical procedure. <i>Nepal Journal of Science and Technology</i> Vol. 7, 2006

scholarly journals Effects of N-Benzyl -9-(2-tetrahydropyranyl and Indole –3-Acetic Acid In Vitro Culture of Bauhinia purpurea L.

2019 ◽  
pp. 238-242
Author(s):  
Belai Meeta Suwal Singh
Keyword(s):  
Analytical Procedure ◽  
Multiple Shoots ◽  
Nodal Explants ◽  
Leguminous Plant ◽  
Ms Medium ◽  
Half Strength ◽  
Bauhinia Purpurea ◽  
Mature Seeds ◽  
Indole 3 Acetic Acid

<p>Bauhinia purpurea L. is a leguminous plant moderate sized tree with multipurpose value. It is distributed in sub-Himalayan tracts. It has been cultivated in the plain region up to the elevation of 1350 m. Mature seeds of Bauhinia purpurea L. were cultured on half strength Murashige and Skoog (1962) (MS) medium. Nodal explants obtained from germinated seedlings were cultured on MS medium containing 0.5 M BAP produced multiple shoots which were used for experimental purposes. Nodal explants obtained from cultured were subcultured on different concentrations of N-Benzyl -9-(2-tetrahydropyranyl) (BPA) and Indole-3acetic acid (IAA). The best proliferation of nodes and shoots were observed on the MS medium supplemented with 0.5 M BPA and 0.1 M IAA. After 8 weeks of culture, the propagated plants were acclimatized and transferred to the sand box containing 1:1 soil and sand. Well rooted plants were then established in the field. All the data collected were worked out statistically with SPSS, a system of analytical procedure.</p>

scholarly journals Effects of Cytokinin on in vitro Propagation of Bauhinia variegata L.

2020 ◽  
Vol 1 (6) ◽  
Author(s):  
Belai Meeta Suwal Singh
Keyword(s):  
In Vitro Propagation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Nodal Cuttings ◽  
Half Strength ◽  
Bauhinia Variegata ◽  
Mature Seeds

Mature seeds of Bauhinia variegata L were cultured on half strength Murashige and Skoog medium. For experimentation, nodal cuttings were used as explants from in vitro growing plants. Cytokinin, N-benzyl-9-(2-tetrahydropyranyl) (BPA), kinetin(6-furfurylaminopurine), zeatin, 6-(4-hydroxy-3-methyl-trans -2-butenyl amino purine), 2- isopentenyl amino purine (2-ip), and benzylaminopurine (BAP) were tested for best propagation. Well grown plants were achieved in medium supplemented with 5 µM BPA and 0.5 µM BAP. The propagated plants were acclimatized very well after transferred to the field.

scholarly journals Pomegranate Micropropagation, Callogenesis and Genetic Integrity Assessment Using Simple Sequence Repeat Markers

2018 ◽  
Vol 11 (1) ◽  
pp. 237
Author(s):  
Tebogo Stimela ◽  
Remmy W. Kasili ◽  
Edward G. Mamati
Keyword(s):  
Acetic Acid ◽  
Simple Sequence Repeat ◽  
Naphthaleneacetic Acid ◽  
Genetic Integrity ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Half Strength ◽  
Indole 3 Acetic Acid ◽  
Simple Sequence

In recent years, the awareness of pomegranate health benefits has grown exponentially; nonetheless the existing propagation methods remain a challenge to supply adequate suitable planting materials needed for commercial production. Micropropagation can lead to mass production of plantlets and callus-mediated in vitro regeneration can open avenues for the use of genetic engineering to improve this crop. The aim of this study was to evaluate appropriate conditions for pomegranate micropropagation, callogenesis and use Simple Sequence Repeat markers to screen for somaclonal variation. Cytokinins (Benzylaminopurine, Kinetin and Thiadiazol-5ylurea) were tested for shoot induction from nodal explants while auxins (1-Naphthaleneacetic acid, Indole-3-butyric acid and Indole-3-acetic acid) were tested for root induction of in vitro regenerated shoots. 1-Naphthaleneacetic acid combined with Benzylaminopurine was assessed for their ability to induce callus from cotyledon and leaf explants. Genetic integrity between mother plant, callus and in vitro regenerated shoots were assessed using eight Simple Sequence Repeat markers. Maximum number of shoots and leaves were obtained on full strength Murashige and Skoog media with 6.9 &micro;M kinetin. The highest number of roots was achieved on half strength Murashige and Skoog media with 4.9 &micro;M Indole-3-butyric acid and the longest root was got on half strength Murashige and Skoog media with 5.3 &micro;M Indole-3-acetic acid. Leaves and cotyledons demonstrated to be potential explants for callus formation at all hormonal combination levels tested. Eight out of 13 amplified alleles were polymorphic. A wider genetic variation was found with similarity coefficient range of 0.46-0.92. More somaclonal variation was in regenerated shoots compared to callus.

scholarly journals Non-symbiotic Seed Germination and In vitro Plant Development of Pholidota articulata

2021 ◽  
Vol 15 ◽  
pp. 44-51
Author(s):  
R. Devendra Prasad ◽  
Shreeti Pradan ◽  
Mukti Ram Poudel ◽  
Bijaya Pant
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Plant Production ◽  
Sustainable Utilization ◽  
Ms Medium ◽  
Natural Habitats ◽  
Half Strength ◽  
Indole 3 Acetic Acid ◽  
Symbiotic Seed Germination

Pholidota articulata is an epiphytic orchid mostly used in ornamental cut/pot flower and in traditional medicine. As it has high ornamental and medicinal values, its population from natural habitats is decreasing, therefore, it is listed in the Appendix-II of Convention on International Trade in Endangered Species (CITES). The objective of the present study is to obtain the in vitro plants of P. articulata from seed culture to conserve its germplasm. The in vitro seed germination was carried out in different strengths of Murashige and Skoog (MS) and Knudson C (KnC) medium supplemented with various plant hormones. On the half-strength of MS medium, seeds were started to germinate after 4 weeks of primary culture and they were developed into protocorms with first leaf primordium earlier than on the other medium. Therefore, in vitro developed protocorms were sub-cultured on the half-strength of MS medium supplemented with different concentrations of 6-benzylaminopurine (BAP), gibberellic acid (GA3) and α-naphtalene acetic acid (NAA). They were successfully developed into shoots on the 1.5 mg/l BAP supplemented half-strength of MS medium. Later, they were inoculated on the half-strength of MS medium supplemented with different concentration of α-napthalene acetic acid (NAA), indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) for the root formation, where IBA supplemented medium was found effective for the development of roots. Thus, this study provides a reliable protocol for non-symbiotic seed germination and plant production, and reveals that seed-derived protocorms are good explants for the in vitro mass propagation for conservation and sustainable utilization in horticulture.

scholarly journals Poinsettia ‘Prestige™ Red’ (Euphorbia pulcherrima) In Vitro Propagation

HortScience ◽  
2010 ◽  
Vol 45 (7) ◽  
pp. 1126-1128
Author(s):  
Dinum Perera ◽  
Brian W. Trader
Keyword(s):  
Growth Rate ◽  
Acetic Acid ◽  
In Vitro Propagation ◽  
Basal Medium ◽  
Euphorbia Pulcherrima ◽  
In Vitro Proliferation ◽  
Slow Growth Rate ◽  
Half Strength ◽  
Indole 3 Acetic Acid

Slow growth rate of plantlets, few micro-shoots per explant, and slow root growth rate are restrictions of in vitro propagation of poinsettia (Euphorbia pulcherrima Willd. ex Koltz). The purpose of this research was to develop an efficient in vitro proliferation technique for poinsettia ‘Prestige™ Red’. Explants (apical buds and axillary buds) placed on Murashige and Skoog (MS) basal medium containing only 6-benzylaminopurine (BA) and combinations of BA and indole-3-acetic acid (IAA) mostly produced red callus, which is productive and some white and gray–green calluses at the base of plantlets after 1 month, whereas explants in a medium without plant growth regulators (PGRs) produced no callus. Addition of IAA into the rooting medium increased rooting efficiency; plantlets grown in half-strength MS salts and vitamins with 28.5 μM IAA initiated rooting 11 days earlier than the plantlets grown with no PGRs. Optimization of PGR concentrations during poinsettia micropropagation helped resolve previous restrictions of in vitro poinsettia proliferation. Chemical names used: 6-benzylaminopurine (BA); indole-3-acetic acid (IAA)

scholarly journals Micropropagation of cranberry (Vaccinium macrocarpon) through shoot tip cultures: Short communication

2011 ◽  
Vol 38 (No. 4) ◽  
pp. 159-162
Author(s):  
J. Sedlák ◽  
F. Paprštein
Keyword(s):  
Acetic Acid ◽  
Woody Plant ◽  
Short Communication ◽  
Callus Formation ◽  
Vaccinium Macrocarpon ◽  
Root Induction ◽  
Multiplication Rate ◽  
Half Strength ◽  
Indole 3 Acetic Acid

The goal of this study was to determine an efficient micropropagation system for cranberry (Vaccinium macrocarpon Ait.). Cranberry cultivar Howes was successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. Anderson&rsquo;s rhododendron medium (AN), half-strength Murashige and Skoog medium (half-MS) and McCown woody plant medium (WPM) containing the cytokinin zeatin in concentrations 0.5, 1 or 2&nbsp;mg/l were tested. Generally, the highest multiplication rate (2.7) was obtained for cv. Howes on AN medium with the concentration 1 mg/l of zeatin. The effects of indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) and naphtalen acetic acid (NAA) on root induction were tested in WPM medium. The percentage of rooting was 100% for NAA, 86% for IBA and 81% for IAA. From the tested variants, auxin NAA also promoted the highest development of good quality roots (more than 5) per shoot without callus formation.

scholarly journals Micropropagation of Acacia chundra (Roxb.) DC.

2008 ◽  
Vol 35 (No. 1) ◽  
pp. 22-26 ◽  
Author(s):  
R. Rout G ◽  
K. Senapati S ◽  
S. Aparajeta
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Basal Medium ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Leguminous Tree ◽  
Half Strength ◽  
Basal Salts ◽  
Indole 3 Acetic Acid

An <I>in vitro</I> propagation of an economic leguminous tree, <I>Acacia chundra</I>, has been standardized. Induction of bud sprout was obtained from shoot tip and nodal explants derived from <I>in vitro</I> grown plants of <I>A. chundra</I> on the Murashige and Skoog (MS) basal medium supplemented with 6-benzylaminopurine (BA) (1.0 mg/l) and 20 mg/l adenine sulfate (Ads). The rate of multiplication was obtained on MS medium supplemented with 1.5 mg/l BA, 0.01 to 0.05 mg/l (indole-3-acetic acid) IAA and 50 mg/l Ads. The multiplication rate varied from 3 to 6 shoots depending on the growth regulators used. Excised shoots were rooted on half-strength MS basal salts supplemented with 0.25 mg/l indole-3-butyric acid (IBA) or IAA and 20 g/l (w/v) sucrose after 10 to 12 days of culture. The micropropagated plantlets have been acclimatized and successfully transferred to soil.

scholarly journals The ectopic expression of Arabidopsis glucosyltransferase UGT74D1 affects leaf positioning through modulating indole-3-acetic acid homeostasis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shanghui Jin ◽  
Bingkai Hou ◽  
Guizhi Zhang
Keyword(s):  
Acetic Acid ◽  
Ectopic Expression ◽  
Leaf Angle ◽  
Kinase Substrate ◽  
Auxin Distribution ◽  
Leaf Tissues ◽  
Photosynthesis Efficiency ◽  
Indole 3 Acetic Acid

AbstractLeaf angle is an important agronomic trait affecting photosynthesis efficiency and crop yield. Although the mechanisms involved in the leaf angle control are intensively studied in monocots, factors contribute to the leaf angle in dicots are largely unknown. In this article, we explored the physiological roles of an Arabidopsis glucosyltransferase, UGT74D1, which have been proved to be indole-3-acetic acid (IAA) glucosyltransferase in vitro. We found that UGT74D1 possessed the enzymatic activity toward IAA glucosylation in vivo and its expression was induced by auxins. The ectopically expressed UGT74D1 obviously reduced the leaf angle with an altered IAA level, auxin distribution and cell size in leaf tissues. The expression of several key genes involved in the leaf shaping and leaf positioning, including PHYTOCHROME KINASE SUBSTRATE (PKS) genes and TEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) genes, were dramatically changed by ectopic expression of UGT74D1. In addition, clear transcription changes of YUCCA genes and other auxin related genes can be observed in overexpression lines. Taken together, our data indicate that glucosyltransferase UGT74D1 could affect leaf positioning through modulating auxin homeostasis and regulating transcription of PKS and TCP genes, suggesting a potential new role of UGT74D1 in regulation of leaf angle in dicot Arabidopsis.

scholarly journals Effects of Variations in Hormonal Treatments Upon In vitro Regeneration Potential in Embryo Explants of Arenga pinnata

2021 ◽  
Vol 17 (5) ◽  
pp. 495-503
Author(s):  
Shamsiah Abdullah ◽  
Siti Nurain Roslan
Keyword(s):  
Acetic Acid ◽  
In Vitro Regeneration ◽  
Hormonal Treatment ◽  
Height Increment ◽  
Regeneration Potential ◽  
In Vitro Method ◽  
Indole 3 Acetic Acid ◽  
Hormonal Treatments

One of the challenges related to propagation of Arenga pinnata is its lengthy period of seed dormancy. In this study, in vitro regeneration was carried out to determine the effect of hormonal treatment on the embryo explant of Arenga pinnata. Embryos were surface sterilized and cultured into different media supplemented with various hormones concentrations and combinations. Each treatment contained of Kinetin (KN) hormone (1.0, 2.0, and 3.0 mg/l) and in combination with indole-3-acetic acid (IAA) of 0.1, 0.2, 0.3 mg/l. The height of plumule and length of radical was observed and recorded. Treatment 8 (3 mg/ml KN + 0.1 mg/ml IAA) showed 59.09% in plumule height increment while treatment 4 (1 mg/ml KN + 0.3 mg/ml IAA) showed the highest radical increments with 93.62%. The knowledge gained in this study consequently helps us to better understand the role of KN and IAA in the in vitro regeneration protocol. Since in vitro method able to produce higher number of in vitro seedlings at one time, it is important to establish the in vitro regeneration protocol for this plant.

Characterization of the indole-3-acetic acid (IAA) biosynthetic pathway in an epiphytic strain of Erwinia herbicola and IAA production in vitro

1996 ◽  
Vol 42 (6) ◽  
pp. 586-592 ◽  
Author(s):  
M. Brandi ◽  
E. M. Clark ◽  
S. E. Lindow
Keyword(s):  
Acetic Acid ◽  
Pseudomonas Syringae ◽  
Pyruvic Acid ◽  
Enzyme Assays ◽  
Erwinia Herbicola ◽  
Iaa Production ◽  
Acid Pathway ◽  
Culture Supernatants ◽  
Indole 3 Acetic Acid

An epiphytic strain of Erwinia herbicola (strain 299R) synthesized indole-3-acetic acid (IAA) from indole-3-pyruvic acid and indole-3-acetaldehyde, but not from indole-3-acetamide and other intermediates of various IAA biosynthetic pathways in enzyme assays. TLC, HPLC, and GC–MS analyses revealed the presence of indole-3-pyruvic acid, indole-3-ethanol, and IAA in culture supernatants of strain 299R. Indole-3-acetaldehyde was detected in enzyme assays. Furthermore, strain 299R genomic DNA shared no homology with the iaaM and iaaH genes from Pseudomonas syringae pv. savastanoi, even in Southern hybridizations performed under low-stringency conditions. These observations strongly suggest that unlike gall-forming bacteria which can synthesize IAA by indole-3-acetamide, the indole-3-pyruvic acid pathway is the primary route for IAA biosynthesis in this plant-associated strain. IAA synthesis in tryptophan-supplemented cultures of strain 299R was over 10-fold higher under nitrogen-limiting conditions, indicating a possible role for IAA production by bacterial epiphytes in the acquisition of nutrients during growth in their natural habitat.Key words: indole-3-acetic acid, Erwinia, tryptophan, indole-3-pyruvic acid, nitrogen.

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