Genomic markers for the study of Ostrinia nubilalis population dynamics and traits conferring resistance to Bacillus thuringiensis (Bt) crystalline toxins

AbstractThis review article starts with the discovery of Bacillus thuringiensis Berliner in Japan at the turn of the century and notes that the observations of the early Japanese workers clearly show that they were aware of the toxin-mediated nature of the activity of B. thuringiensis toward insect larvae. The early work in Europe with B. thuringiensis against Ostrinia nubilalis (Hubner) showed that the bacterium had promise as a microbial control agent. The commercial development of B. thuringiensis in France in the late 1930s, and in Eastern Europe and the United States in the 1950s, is traced.

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Survival of Ostrinia nubilalis (Hübner) After Exposure to Bacillus thuringiensis Berliner Encapsulated in Flour Matrices2

Journal of Entomological Science ◽

10.18474/0749-8004-29.4.496 ◽

1994 ◽

Vol 29 (4) ◽

pp. 496-508 ◽

Cited By ~ 1

Author(s):

Michael R. McGuire ◽

Robert L. Gillespie ◽

Baruch S. Shasha

Keyword(s):

Bacillus Thuringiensis ◽

Ostrinia Nubilalis ◽

European Corn Borer ◽

Significant Loss ◽

The Other ◽

Field Site ◽

Corn Flour ◽

Corn Borer ◽

Natural Infestation ◽

Corn Plants

Two types of pregelatinized corn flour were used to produce granules containing Bacillus thuringiensis Berliner subsp. kurstaki and various additives for control of the European corn borer, Ostrinia nubilalis (Hübner), in the whorl of corn plants. Laboratory-reared larvae were applied to corn whorls in the greenhouse and field, and a high natural infestation occurred at one field site (Champaign). In the greenhouse and at all three field sites, five of these formulations were just as effective as Dipel 10G, a commercially available B. thuringiensis product, for control of European corn borer larvae. In all greenhouse studies and at one of the three field sites (Champaign), the dose of B. thuringiensis could be reduced by as much as 75% when a phagostimulant was added to flour granules without significant loss of corn borer control. The phagostimulant dose response was not observed at the other two field sites in which larval infestations were relatively low. Flour type had no significant effect on European corn borer control under greenhouse and field conditions. Greenhouse evaluations provided results significantly similar to results from two of the field sites indicating the usefulness of the technique. The data presented highlight the versatility and potential for using novel formulation techniques for enhancing the efficacy of B. thuringiensis.

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Simulation Model of the Population Dynamics of Ostrinia nubilalis (Lepidoptera: Pyralidae) in Maize

Environmental Entomology ◽

10.1093/ee/17.6.969 ◽

1988 ◽

Vol 17 (6) ◽

pp. 969-976 ◽

Cited By ~ 17

Author(s):

David W. Onstad

Keyword(s):

Population Dynamics ◽

Simulation Model ◽

Ostrinia Nubilalis ◽

Lepidoptera Pyralidae

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Novel Vip3-Related Protein from Bacillus thuringiensis

Applied and Environmental Microbiology ◽

10.1128/aem.71.10.6276-6281.2005 ◽

2005 ◽

Vol 71 (10) ◽

pp. 6276-6281 ◽

Cited By ~ 43

Author(s):

Cécile Rang ◽

Patricia Gil ◽

Nathalie Neisner ◽

Jeroen Van Rie ◽

Roger Frutos

Keyword(s):

Amino Acids ◽

Bacillus Thuringiensis ◽

Ostrinia Nubilalis ◽

Repeated Sequence ◽

Proteolytic Processing ◽

Related Protein ◽

Terminal Part ◽

Larvicidal Effect ◽

Charged Amino Acids ◽

Novel Protein

ABSTRACT A novel vip3-related gene was identified in Bacillus thuringiensis. This novel gene is 2,406 bp long and codes for a 91-kDa protein (801 amino acids). This novel protein exhibits between 61 and 62% similarity with Vip3A proteins and is designated Vip3Ba1. Vip3Ba1 has several specific features. Differences between Vip3Ba1 and the Vip3A proteins are spread throughout the sequence but are more frequent in the C-terminal part from amino acid 456 onward. The regions containing the two proteolytic processing sites, which are highly conserved among the Vip3A toxins, are markedly different in Vip3Ba1. The pattern DCCEE (Asp Cys Cys Glu Glu) is repeated four times between position 463 and 483 in Vip3Ba1, generating the sequence 463-DCCEEDCCEEDCCEEDCCEE-483. This sequence, which is rich in negatively charged amino acids, also contains 73% of the cysteines present in Vip3Ba1. This repeated sequence is not present in Vip3A proteins. The Vip3Ba1protein was produced in Escherichia coli and tested against Ostrinia nubilalis and Plutella xylostella, and it generated significant growth delays but had no larvicidal effect, indicating that its host range might be different than that of Vip3A proteins.

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