Survival of Ostrinia nubilalis (Hübner) After Exposure to Bacillus thuringiensis Berliner Encapsulated in Flour Matrices2

1994 ◽  
Vol 29 (4) ◽  
pp. 496-508 ◽  
Author(s):  
Michael R. McGuire ◽  
Robert L. Gillespie ◽  
Baruch S. Shasha

Two types of pregelatinized corn flour were used to produce granules containing Bacillus thuringiensis Berliner subsp. kurstaki and various additives for control of the European corn borer, Ostrinia nubilalis (Hübner), in the whorl of corn plants. Laboratory-reared larvae were applied to corn whorls in the greenhouse and field, and a high natural infestation occurred at one field site (Champaign). In the greenhouse and at all three field sites, five of these formulations were just as effective as Dipel 10G, a commercially available B. thuringiensis product, for control of European corn borer larvae. In all greenhouse studies and at one of the three field sites (Champaign), the dose of B. thuringiensis could be reduced by as much as 75% when a phagostimulant was added to flour granules without significant loss of corn borer control. The phagostimulant dose response was not observed at the other two field sites in which larval infestations were relatively low. Flour type had no significant effect on European corn borer control under greenhouse and field conditions. Greenhouse evaluations provided results significantly similar to results from two of the field sites indicating the usefulness of the technique. The data presented highlight the versatility and potential for using novel formulation techniques for enhancing the efficacy of B. thuringiensis.

1999 ◽  
Vol 34 (3) ◽  
pp. 273-285
Author(s):  
Richard L. Ridgway ◽  
Robert R. Farrar

Five commercial granular formulations of Bacillus thuringiensis Berliner marketed for controlling the European corn borer, Ostrinia nubilalis (Hübner), were compared for insecticidal activity using treated discs of bean leaves. Three formulations, Dipel 10G®, Full-Bac ECBG™, and Strike BT®, were similar in terms of both mortality and speed of kill. A formulation containing a strain of B. thuringiensis developed by plasmid fusion, Condor G®, caused mortality similar to the other three formulations, but the speed of kill was slower. A fifth formulation containing a B. thuringiensis toxin produced by Pseudomonas fluorescens Migula as result of a gene transfer, M-Peril™, caused substantially less mortality than any of the other formulations. An experimental water dispersible formulation, based on a previously developed granular matrix formulation containing B. thuringiensis and a nutrient-based phagostimulant, caused significantly higher mortality of the European corn borer than a similar formulation without the phagostimulant. Simulated field studies were conducted to study the effects of the phagostimulant on feeding and protection of B. thuringiensis from ultraviolet (UV) light. Bean plants treated with B. thuringiensis and the phagostimulant were exposed to different UV regimes outdoors under canopies made of specialized acrylic plastics and then infested with larvae of the corn earworm, Helicoverpa zea (Boddie). A significant interaction between the UV regimes and the phagostimulant was found, indicating that the phagostimulant acted both as a feeding stimulant and as a UV protectant to enhance the activity of B. thuringiensis.


HortScience ◽  
2004 ◽  
Vol 39 (6) ◽  
pp. 1336-1338 ◽  
Author(s):  
James D. Frantz ◽  
Jeffrey Gardner ◽  
Michael P. Hoffmann ◽  
Molly M. Jahn

A replicated greenhouse evaluation of a range of commercial and noncommercial (Capsicum spp.) accessions for resistance to european corn borer (ECB) [Ostrinia nubilalis (Hubner)] was conducted. Percentage of fruit damaged was observed among 29 accessions four weeks after plants were artificially infested with ECB egg masses. Small-fruited peppers generally showed lower levels of damage, while large-fruited peppers were the most susceptible. Genotypes with elongate fruit were less damaged than those with bell-shaped fruit. Resistance to fruit damage was also associated with increasing pungency level, with two notable exceptions. The pungent genotype `Large Red Thick Cayenne' was significantly more susceptible than many of the other pungent accessions tested. The relative susceptibility of this accession may be related to large fruit size. The nonpungent pepper `Corno di Toro' showed significantly lower percent fruit damage than other nonpungent peppers including `Banana Supreme' with roughly similar fruit size, ranking amidst highly pungent peppers such as `Red Scotch Bonnet'. These results confirm that resistance to ECB can be identified in nonpungent Capsicum genotypes and demonstrate that pungency is not always correlated with ECB damage. Reported sources of aphid resistance or tolerance showed good levels of ECB resistance, but interpretation of these results was confounded by the presence of pungency.


1995 ◽  
Vol 30 (1) ◽  
pp. 29-42 ◽  
Author(s):  
Robert R. Farrar ◽  
Richard L. Ridgway

To help improve control of insect pests with microbial insecticides, we investigated the interactions of four commercial, nutrient-based phagostimulants (Pheast [AgriSense], Coax [CCT Corp.], Gusto [Atochem North America, Inc.], and Entice [Custom Chemicides] with Bacillus thuringiensis Berliner and four lepidopterous insect pests (gypsy moth, Lymantria dispar [L.] [Lymantriidae]; corn earworm, Helicoverpa zea [Boddie] [Noctuidae]; European corn borer, Ostrinia nubilalis [Hübner] [Pyralidae]; and diamondback moth, Plutella xylostella [L.] [Plutellidae]). Comparisons were made of treated foliage in Petri dishes in the laboratory and of sprayed whole plants in a greenhouse. In general, phagostimulants increased mortality of all species tested, but no consistent differences among phagostimulants were found for any species. Food consumption was generally lower on the treatments that contained phagostimulants causing the highest rates of mortality, possibly as a result of more rapid ingestion of a lethal dose on these treatments. Reduced rates of feeding by insects on treatments with B. thuringiensis alone were seen, probably due in part to intoxication and, possibly, to behavioral effects as well. Indications of potentially significant interactions between host plants and both B. thuringiensis and phagostimulants also were seen.


2009 ◽  
Vol 65 (10) ◽  
pp. 1071-1081 ◽  
Author(s):  
André LB Crespo ◽  
Terence A Spencer ◽  
Analiza P Alves ◽  
Richard L Hellmich ◽  
Erin E Blankenship ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document