In vitro and field responses of various active ingredients to Fusarium proliferatum species which causes Fusarium root rot disease in Indian mulberry (Morinda officinalis How.) in Thai Nguyen

Abstract Background Fusarium root rot disease in Indian mulberry (Morinda officinalis How.) (FRRBK), caused by Fusarium proliferatum (FP), is widespread and responsible for serious economic losses in Viet Nam. The efficacy of a new bio-product named MICROTECH-1(NL) is compared with other commercial products for suppression of FP under in vitro, pot, nursery as well as in the field conditions. Results In in vitro antagonistic assay, MICROTECH-1(NL) significantly inhibited the mycelial growth of FP (72.38%). Under pot conditions, the efficacy of all the bio-products was significantly higher when applied prior to pathogen inoculation. The disease severity of treatments with double application of MICROTECH-1(NL) (applied both in the nursery and in the pot soil) was only 15.56%, significantly lower than control (80%). Thus, the application of MICROTECH-1(NL) significantly reduced the incidence of FP and markedly increased the number of plant beneficial bacteria and actinobacteria in rhizoplane of M. officinalis compared to untreated control. In the field conditions, double application of MICROTECH-1(NL) (both in the nursery and in the field soils) significantly decreased disease severity in comparison to single application in nursery or field. Conclusion The most effective treatment was double application of MICROTECH-1(NL), which significantly reduced the disease severity and FP population in roots of M. officinalis and increased the population of plant beneficial microbes.

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First report of molecular characterisation of Fusarium proliferatum associated with root rot disease of Indian mulberry (Morinda officinalis How.) in Viet Nam

Archives of Phytopathology and Plant Protection ◽

10.1080/03235408.2019.1603351 ◽

2019 ◽

Vol 52 (1-2) ◽

pp. 200-217 ◽

Cited By ~ 1

Author(s):

Duong Thi Nguyen ◽

Nguyen Chi Hieu ◽

Hoang Thi Bich Thao ◽

Trinh Xuan Hoat

Keyword(s):

Root Rot ◽

Fusarium Proliferatum ◽

Viet Nam ◽

Molecular Characterisation ◽

First Report ◽

Root Rot Disease ◽

Indian Mulberry ◽

Rot Disease

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Bacillus megaterium-Mediated Synthesis of Selenium Nanoparticles and Their Antifungal Activity against Rhizoctonia solani in Faba Bean Plants

Journal of Fungi ◽

10.3390/jof7030195 ◽

2021 ◽

Vol 7 (3) ◽

pp. 195

Author(s):

Amr H. Hashem ◽

Amer M. Abdelaziz ◽

Ahmed A. Askar ◽

Hossam M. Fouda ◽

Ahmed M. A. Khalil ◽

...

Keyword(s):

Antifungal Activity ◽

Vicia Faba ◽

Root Rot ◽

Antifungal Agents ◽

Damping Off ◽

Growth Promoters ◽

Root Rot Disease ◽

Rot Disease

Rhizoctonia root-rot disease causes severe economic losses in a wide range of crops, including Vicia faba worldwide. Currently, biosynthesized nanoparticles have become super-growth promoters as well as antifungal agents. In this study, biosynthesized selenium nanoparticles (Se-NPs) have been examined as growth promoters as well as antifungal agents against Rhizoctonia solani RCMB 031001 in vitro and in vivo. Se-NPs were synthesized biologically by Bacillus megaterium ATCC 55000 and characterized by using UV-Vis spectroscopy, XRD, dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. TEM and DLS images showed that Se-NPs are mono-dispersed spheres with a mean diameter of 41.2 nm. Se-NPs improved healthy Vicia faba cv. Giza 716 seed germination, morphological, metabolic indicators, and yield. Furthermore, Se-NPs exhibited influential antifungal activity against R. solani in vitro as well as in vivo. Results revealed that minimum inhibition and minimum fungicidal concentrations of Se-NPs were 0.0625 and 1 mM, respectively. Moreover, Se-NPs were able to decrease the pre-and post-emergence of R. solani damping-off and minimize the severity of root rot disease. The most effective treatment method is found when soaking and spraying were used with each other followed by spraying and then soaking individually. Likewise, Se-NPs improve morphological and metabolic indicators and yield significantly compared with infected control. In conclusion, biosynthesized Se-NPs by B. megaterium ATCC 55000 are a promising and effective agent against R. solani damping-off and root rot diseases in Vicia faba as well as plant growth inducer.

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