scholarly journals Biological control of fusarium root rot of Indian mulberry (Morinda officinalis How.) with consortia of agriculturally important microorganisms in Viet Nam

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Duong Thi Nguyen ◽  
Nguyen Chi Hieu ◽  
Nguyen Viet Hung ◽  
Hoang Thi Bich Thao ◽  
Chetan Keswani ◽  
...  
Keyword(s):  
Disease Severity ◽  
Root Rot ◽  
Field Conditions ◽  
Economic Losses ◽  
Viet Nam ◽  
Beneficial Bacteria ◽  
Fusarium Root Rot ◽  
Indian Mulberry ◽  
Rot Disease

Abstract Background Fusarium root rot disease in Indian mulberry (Morinda officinalis How.) (FRRBK), caused by Fusarium proliferatum (FP), is widespread and responsible for serious economic losses in Viet Nam. The efficacy of a new bio-product named MICROTECH-1(NL) is compared with other commercial products for suppression of FP under in vitro, pot, nursery as well as in the field conditions. Results In in vitro antagonistic assay, MICROTECH-1(NL) significantly inhibited the mycelial growth of FP (72.38%). Under pot conditions, the efficacy of all the bio-products was significantly higher when applied prior to pathogen inoculation. The disease severity of treatments with double application of MICROTECH-1(NL) (applied both in the nursery and in the pot soil) was only 15.56%, significantly lower than control (80%). Thus, the application of MICROTECH-1(NL) significantly reduced the incidence of FP and markedly increased the number of plant beneficial bacteria and actinobacteria in rhizoplane of M. officinalis compared to untreated control. In the field conditions, double application of MICROTECH-1(NL) (both in the nursery and in the field soils) significantly decreased disease severity in comparison to single application in nursery or field. Conclusion The most effective treatment was double application of MICROTECH-1(NL), which significantly reduced the disease severity and FP population in roots of M. officinalis and increased the population of plant beneficial microbes.

scholarly journals Characterization of endophytic yeast and its suppressive effect on root rotting fungi of tomato under neem cake soil amendment

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Ayesha Jamal ◽  
Hafiza Farhat ◽  
Faizah Urooj ◽  
Afshan Rahman ◽  
Muhammed Irfan ◽  
...  
Keyword(s):  
Root Rot ◽  
Soil Amendment ◽  
Biocontrol Agent ◽  
Suppressive Effect ◽  
Economic Losses ◽  
Tomato Plants ◽  
Neem Cake ◽  
Root Rot Disease ◽  
Rot Disease

Abstract Background The exposure of crops to a variety of fungal and bacterial pathogens leads to huge economic losses. Different strategies are being adapted to control these diseases among which the application of chemicals fungicide is common. However, these chemicals are posing a serious threat to the environment. For biological management of root rot disease of tomato and better fruit quality, studies were conducted on the possible use of endophytic yeast as a biocontrol agent. Results Endophytic yeasts were isolated from healthy plants and identified. Identification of selected isolates was confirmed on the basis of 18S rDNA gene sequencing. They were evaluated for suppressive effect on root rotting fungi in vitro and also in vivo on tomato plants, used alone or under neem cake soil amendment. Seventy-six isolates of yeasts were evaluated against root rotting fungi Fusarium oxysporum, F. solani, Rhizoctonia solani and Macrophomina phaseolina using dual culture plate assay. Seventy-five isolates were found to suppress radial growth of F. oxysporum, F. solani and M. phaseolina by producing zones of inhibition or lysing the fungal hyphae. However, none of the isolates was found to inhibit R. solani in vitro. Most of the isolates also caused nematicidal activity at varying degree against Meloidogyne javanica. All test isolates produced indole acetic acid in vitro and solubilized phosphorus. In pots and field plot experiments, test isolates of yeasts were able to suppress root rotting fungi on tomato in natural soil and soil amended with neem cake with enhancement of growth of tomato plants. Yeasts were also found to ameliorate the plant resistance through enhancing polyphenolic contents, salicylic acid and antioxidant activity. Conclusions Endophytic yeasts were found effective against root rot disease of tomato and could be used as a potential biocontrol agent for the management of soil-borne diseases of tomatoes.

scholarly journals Diagnosis and control of the cucumber root rot causes using some chemical and biological agents: تشخيص ومكافحة مسببات مرض تعفن جذور نبات الخيار باستعمال بعض العوامل الكيميائية والأحيائية

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Safaa Neamat Hussein
Keyword(s):  
Root Rot ◽  
Disease Incidence ◽  
Positive Control ◽  
Negative Control ◽  
Bacterial Isolates ◽  
Beneficial Bacteria ◽  
Rot Disease ◽  
And Control ◽  
Gm Plant

This research was aimed to identify the causal agent of the root rot disease of cucumber in some provinces of Iraq, and evaluate its pathogenicity and control it using beneficial bacteria of Serratia odorifera (So) and Enterobacter cloacae (Ec) which isolated from the rhizosphere of healthy cucumber plants, and using silicon (Si) and copper (Cu). The fungus Fusarium solani was predominant, while its percentage of appearance was 80.0% with frequency of 46.2%, Isolate Bfs- 1 was most virulent which prevent germination of the cucumber's seed overall in vitro compared to the control. The bacterial isolates D6 and Q10 exhibited 100% percentage of inhibition against the pathogen in vitro. Results of bacterial isolates identification using Vitiq2 Compact System showed that they belong to S. odorifera and E. cloacae respectively. Under greenhouse conditions the quadrant treatment (So+Ec+Si+Cu) was superior in controlling the disease, while it exhibited 100% percentage of seed germination compared to the negative control (Fungus alone) which achieved 58.0% and the percentage of disease incidence and severity were 9.0%, 3.5% respectively compared to the negative control which were 90.0%, 66.5% respectively. And the quadrant treatment exhibited significant increase of plant growth criteria represented by the dry width of plant, while it achieved 2.69 gm/plant compared to negative and positive control which were 0.85, 2.16 gm/plant respectively.

scholarly journals Trichoderma spp. decrease Fusarium root rot in common bean

2012 ◽  
Vol 38 (4) ◽  
pp. 334-336 ◽  
Author(s):  
Hudson Teixeira ◽  
Trazilbo José de Paula Júnior ◽  
Rogério Faria Vieira ◽  
Marcelo Barreto da Silva ◽  
Camila Geovana Ferro ◽  
...  
Keyword(s):  
Common Bean ◽  
Disease Severity ◽  
Root Rot ◽  
Seed Treatment ◽  
Seedling Emergence ◽  
Field Conditions ◽  
Fusarium Root Rot ◽  
Trichoderma Spp ◽  
Commercial Products ◽  
Control Application

The effectiveness of six Trichoderma-based commercial products (TCP) in controlling Fusarium root rot (FRR) in common bean was assessed under field conditions. Three TCP, used for seed treatment or applied in the furrow, increased seedling emergence as much as the fungicide fludioxonil. FRR incidence was not affected, but all TCP and fludioxonil reduced the disease severity, compared to control. Application of Trichoderma-based products was as effective as that of fludioxonil in FRR management.

Management of Root Rot (Rhizoctonia solani) of Fenugreek through Newer Combined Formulations of Fungicides

2020 ◽  
Author(s):  
Shankar Lal Yadav ◽  
R. P. Ghasolia ◽  
Jitendra Sharma
Keyword(s):  
Rhizoctonia Solani ◽  
Root Rot ◽  
Seed Treatment ◽  
Disease Incidence ◽  
In Vitro Study ◽  
Field Conditions ◽  
Alternative Source ◽  
Root Rot Disease ◽  
Rot Disease

Background: Root rot disease of fenugreek caused by Rhizoctonia solani has become a severe menace to the growers of Rajasthan as well as India. This pathogen survives in soil, causes damping off and root rot symptoms and responsible nearly for 50 per cent disease incidence and yield losses. Methods: During 2016-17 to 2017-18 cropping seasons, this study was carried out with the aim to manage the disease by means of using six fungicides including newer formulations (hexaconazole and tebuconazole + trifloxystrobin) under in vitro and field conditions. Result: The result of in vitro study with tebuconazole + trifloxystrobin was found to be most fungitoxic and inhibited mycelial growth cent per cent at 200 and 500 ppm concentrations. Seed treatment with tebuconazole + trifloxystrobin (@ 0.2%) was found highly effective in reducing disease incidence (83.12%) and in increasing seed yield (84.71%) under field conditions. Thus, it can be concluded that the use of newer combined formulation as seed treatment before sowing provide us alternative source to manage root rot disease of fenugreek.

scholarly journals Bacillus megaterium-Mediated Synthesis of Selenium Nanoparticles and Their Antifungal Activity against Rhizoctonia solani in Faba Bean Plants

2021 ◽  
Vol 7 (3) ◽  
pp. 195
Author(s):  
Amr H. Hashem ◽  
Amer M. Abdelaziz ◽  
Ahmed A. Askar ◽  
Hossam M. Fouda ◽  
Ahmed M. A. Khalil ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Vicia Faba ◽  
Root Rot ◽  
Antifungal Agents ◽  
Damping Off ◽  
Growth Promoters ◽  
Root Rot Disease ◽  
Rot Disease

Rhizoctonia root-rot disease causes severe economic losses in a wide range of crops, including Vicia faba worldwide. Currently, biosynthesized nanoparticles have become super-growth promoters as well as antifungal agents. In this study, biosynthesized selenium nanoparticles (Se-NPs) have been examined as growth promoters as well as antifungal agents against Rhizoctonia solani RCMB 031001 in vitro and in vivo. Se-NPs were synthesized biologically by Bacillus megaterium ATCC 55000 and characterized by using UV-Vis spectroscopy, XRD, dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. TEM and DLS images showed that Se-NPs are mono-dispersed spheres with a mean diameter of 41.2 nm. Se-NPs improved healthy Vicia faba cv. Giza 716 seed germination, morphological, metabolic indicators, and yield. Furthermore, Se-NPs exhibited influential antifungal activity against R. solani in vitro as well as in vivo. Results revealed that minimum inhibition and minimum fungicidal concentrations of Se-NPs were 0.0625 and 1 mM, respectively. Moreover, Se-NPs were able to decrease the pre-and post-emergence of R. solani damping-off and minimize the severity of root rot disease. The most effective treatment method is found when soaking and spraying were used with each other followed by spraying and then soaking individually. Likewise, Se-NPs improve morphological and metabolic indicators and yield significantly compared with infected control. In conclusion, biosynthesized Se-NPs by B. megaterium ATCC 55000 are a promising and effective agent against R. solani damping-off and root rot diseases in Vicia faba as well as plant growth inducer.

scholarly journals Improving the Biocontrol Potential of Bacterial Antagonists with Salicylic Acid against Brown Rot Disease and Impact on Nectarine Fruits Quality

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 209
Author(s):  
Nadia Lyousfi ◽  
Rachid Lahlali ◽  
Chaimaa Letrib ◽  
Zineb Belabess ◽  
Rachida Ouaabou ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Disease Severity ◽  
Mycelial Growth ◽  
Brown Rot ◽  
Antagonistic Bacteria ◽  
Biological Treatments ◽  
Rot Disease ◽  
The Impact

The main objective of this study was to evaluate the ability of both antagonistic bacteria Bacillus amyloliquefaciens (SF14) and Alcaligenes faecalis (ACBC1) used in combination with salicylic acid (SA) to effectively control brown rot disease caused by Monilinia fructigena. Four concentrations of salicylic acid (0.5%, 2%, 3.5%, and 5%) were tested under in vitro and in vivo conditions. Furthermore, the impact of biological treatments on nectarine fruit parameters’ quality, in particular, weight loss, titratable acidity, and soluble solids content, was evaluated. Regardless of the bacterium, the results indicated that all combined treatments displayed a strong inhibitory effect on the mycelial growth of M. fructigena and disease severity. Interestingly, all SA concentrations significantly improved the biocontrol activity of each antagonist. The mycelial growth inhibition rate ranged from 9.79% to 88.02% with the highest reduction rate recorded for bacterial antagonists in combination with SA at both concentrations of 0.5% and 3.5%. The in vivo results confirmed the in vitro results with a disease severity varying from 0.00% to 51.91%. A significant biocontrol improvement was obtained with both antagonistic bacteria when used in combination with SA at concentrations of 0.5% and 2%. The lowest disease severity observed with ACBC1 compared with SF14 is likely due to a rapid adaptation and increase of antagonistic bacteria population in wounded sites. The impact of all biological treatments revealed moderate significant changes in the fruit quality parameters with weight loss for several treatments. These results suggest that the improved disease control of both antagonistic bacteria was more likely directly linked to both the inhibitory effects of SA on pathogen growth and induced fruit resistance.

scholarly journals Application of Bacillus pumilus isolates for management of black rot disease in strawberry

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Farid Abd-El-Kareem ◽  
Ibrahim E. Elshahawy ◽  
Mahfouz M. M. Abd-Elgawad
Keyword(s):  
Root Rot ◽  
Bacillus Pumilus ◽  
Field Conditions ◽  
Black Rot ◽  
Bacterial Isolates ◽  
Black Root Rot ◽  
Root Rot Disease ◽  
Growth Area ◽  
Rot Disease ◽  
The Impact

Abstract Background Black root rot of strawberry plants caused by Rhizoctonia solani, Fusarium solani, and Pythium sp. is a serious disease in Egypt. Biocontrol agents have frequently proved to possess paramount and safe tools against many diseases. The impact of soil treatments with 3 Bacillus pumilus isolates on black root rot disease of strawberry plants caused by R. solani, F., and Pythium sp. under laboratory and field conditions was examined herein on the commonly used ‘Festival’ strawberry cultivar. To increase the bacterial adhesion and distribution on the roots, each seedling was dipped in bacterial cell suspension at 1 × 108 colony-forming units/ml of each separate bacterial isolate for 30 min then mixed with 5% Arabic gum. Results The tested B. pumilus isolates significantly reduced the growth area of these 3 fungi. The two bacterial isolates Nos. 2 and 3 reduced the growth area by more than 85.2, 83.6, and 89.0% for R. solani, F. solani, and Pythium sp., respectively. Likewise, the 3 bacterial isolates significantly (P ≤ 0.05) inhibited the disease under field conditions. Isolates Nos. 2 and 3 suppressed the disease incidence by 64.4 and 68.9% and disease severity by 65.3 and 67.3%, respectively. The fungicide Actamyl had effect similar to that of the 2 isolates. B. pumilus isolates significantly enhanced growth parameters and yields of strawberry plants; isolates Nos. 2 and 3 raised the yield by 66.7 and 73.3%, respectively. Conclusions Bacillus pumilus isolates could effectively manage the black rot disease in strawberry herein. Due to the significant impact of the root rot disease on strawberry yield, B. pumilus should be further tested to manage the disease on strawberry on large scale in Egypt.

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