scholarly journals A Sandwich ELISA for the Determination of Beef Meat Content in Processed Foods

2009 ◽  
Vol 15 (6) ◽  
pp. 613-618 ◽  
Author(s):  
Satoshi KOTOURA ◽  
Yukie MURAKAMI-YAMAGUCHI ◽  
Miyuki NAKAMURA ◽  
Kiyotaka MIAKE ◽  
Masaaki SUGIYAMA ◽  
...  
Keyword(s):  
Sandwich Elisa ◽  
Processed Foods ◽  
Beef Meat ◽  
Meat Content

scholarly journals Enzyme-Linked Immunosorbent Assay Kit for the Determination of Soybean Protein in Processed Foods: Interlaboratory Evaluation

2010 ◽  
Vol 93 (1) ◽  
pp. 243-248 ◽  
Author(s):  
Shinobu Sakai ◽  
Reiko Adachi ◽  
Hiroshi Akiyama ◽  
Reiko Teshima ◽  
Naoki Morishita ◽  
...  
Keyword(s):  
Soybean Protein ◽  
Sandwich Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Foods ◽  
Adzuki Bean ◽  
Soybean Proteins ◽  
Elisa Kit ◽  
Number Of Patients ◽  
High Level

Abstract The labeling of foods containing ingredients derived from soybean is recommended in Japan because of an increasing number of patients who are allergic to soybeans. To ensure proper labeling, a novel sandwich ELISA kit for the determination of soybean protein in processed foods (FASTKIT Ver. II, Soybean, Nippon Meat Packers, Inc.; soy kit) has been developed. Five types of incurred samples (model processed foods: rice gruel, sausage, sweet adzuki bean soup, sweet potato cake, and tomato sauce) containing 10 g soybean soluble protein/g food were prepared for use in interlaboratory evaluations of the soy kit. The soy kit displayed a sufficient RSDR value (interlaboratory precision: 9.313.4 RSDR) and a high level of recovery (97114) for all the incurred samples. The RSDr value for the incurred samples was mostly <4.8. The results of this interlaboratory evaluation suggest that the soy kit can be used as a precise and reliable tool for the determination of soybean proteins in processed foods.

scholarly journals Determination of Walnut Protein in Processed Foods by Enzyme-Linked Immunosorbent Assay: Interlaboratory Study

2010 ◽  
Vol 93 (4) ◽  
pp. 1255-1261 ◽  
Author(s):  
Shinobu Sakai ◽  
Reiko Adachi ◽  
Hiroshi Akiyama ◽  
Reiko Teshima ◽  
Hirotoshi Doi ◽  
...  
Keyword(s):  
Sandwich Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Foods ◽  
Food Allergens ◽  
2S Albumin ◽  
Elisa Kit ◽  
Tree Nuts ◽  
Relative Standard ◽  
High Level

Abstract Because food allergens from tree nuts, including walnuts, are a frequent cause of adverse food reactions for allergic patients, the labeling of foods containing ingredients derived from tree nuts is required in numerous countries. According to Japanese regulations, the labeling of food products containing walnuts is recommended. To ensure proper labeling, a novel sandwich ELISA kit for the determination of walnut protein in processed foods (Walnut Protein [2S-Albumin] Kit; Morinaga Institute of Biological Science, Inc.; walnut kit) has been developed. We prepared seven types of incurred samples (model processed foods: biscuits, bread, sponge cake, orange juice, jelly, chicken meatballs, and rice gruel) containing 10 g walnut soluble protein/g of food for use in interlaboratory evaluations of the walnut kit. The walnut kit displayed sufficient reproducibility relative standard deviations (interlaboratory precision: 5.89.9 RSDR) and a high level of recovery (81119) for all the incurred samples. All the repeatability relative standard deviation (RSDr) values for the incurred samples that were examined were less than 6.0. The results of this interlaboratory evaluation suggested that the walnut kit could be used as a precise and reliable tool for determination of walnut protein in processed foods.

Establishment of a sandwich ELISA for the determination of beef content in processed foods by using monoclonal antibodies to myoglobin

2012 ◽  
Vol 23 (3) ◽  
pp. 289-301 ◽  
Author(s):  
Satoshi Kotoura ◽  
Yukie Murakami-Yamaguchi ◽  
Kumiko Kizu ◽  
Miyuki Nakamura ◽  
Hidetaka Fuchu ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Sandwich Elisa ◽  
Processed Foods

Determination of Cardiac Disease Biomarker by Plasmonic Sandwich‐ELISA

2020 ◽  
Author(s):  
Weirong Wei ◽  
Yinyan Tang ◽  
Huimin He ◽  
Subash C.B. Gopinath ◽  
Lingling Wang
Keyword(s):  
Cardiac Disease ◽  
Sandwich Elisa ◽  
Disease Biomarker

An immunological determination of somatostatin in pharmaceutical by sandwich ELISA based on IgY and polyclonal antibody

2019 ◽  
Vol 145 ◽  
pp. 532-538
Author(s):  
Yingshan Chen ◽  
Yuxin Liang ◽  
Rui Lv ◽  
Nana Xia ◽  
Tianjian Xue ◽  
...  
Keyword(s):  
Polyclonal Antibody ◽  
Sandwich Elisa

scholarly journals Interlaboratory Evaluation of Two Enzyme-Linked Immunosorbent Assay Kits for the Determination of Crustacean Protein in Processed Foods

2008 ◽  
Vol 91 (1) ◽  
pp. 123-129 ◽  
Author(s):  
Shinobu Sakai ◽  
Rieko Matsuda ◽  
Reiko Adachi ◽  
Hiroshi Akiyama ◽  
Tamio Maitani ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Immunosorbent Assay ◽  
Soluble Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Foods ◽  
Relative Standard ◽  
High Level

Abstract The labeling of foods containing material derived from crustaceans such as shrimp and crab is to become mandatory in Japan because of increases in the number of allergy patients. To ensure proper labeling, 2 novel sandwich enzyme-linked immunosorbent assay (ELISA) kits for the determination of crustacean protein in processed foods, the N kit (Nissui Pharmaceutical Co., Ltd, Ibaraki, Japan) and the M kit (Maruha Nichiro Holdings, Inc., Ibaraki, Japan), have been developed. Five types of model processed foods containing 10 and/or 11.9 g/g crustacean soluble protein were prepared for interlaboratory evaluation of the performance of these kits. The N kit displayed a relatively high level of reproducibility relative standard deviation (interlaboratory precision; 4.08.4 RSDR) and sufficient recovery (6586) for all the model processed foods. The M kit displayed sufficient reproducibility (17.620.5 RSDR) and a reasonably high level of recovery (82103). The repeatability relative standard deviation (RSDr) values regarding the detection of crustacean proteins in the 5 model foods were mostly <5.1 RSDr for the N kit and 9.9 RSDr for the M kit. In conclusion, the results of this interlaboratory evaluation suggest that both these ELISA kits would be very useful for detecting crustacean protein in processed foods.

Effect of Residual Ascorbate on Determination of Nitrite in Commercial Cured Meat Products

1984 ◽  
Vol 67 (4) ◽  
pp. 692-697
Author(s):  
Jay B Fox ◽  
Robert C Doerr ◽  
Robert Gates
Keyword(s):  
Meat Products ◽  
Processing Conditions ◽  
Griess Reagent ◽  
Meat Content ◽  
Cured Meat

Abstract Residual ascorbate in cured meat slurries results in different amounts of pigment being produced from different Griess reagent combinations. The phenomenon was used to study residual ascorbate in commercial cured meat products which had a variety of textures, acidities, moisture and meat content, fat, homogeneity, initial nitrite, and processing conditions. Diluting and heating the samples according to the AOAC procedure did not completely eliminate the ascorbate interference, but making the sample alkaline did. Determining nitrite separately in supernate and precipitate from the first dilution showed the effect of heating to be the elimination of interferences and solubilization or extraction of nitrite from the precipitate.

Multiresidue Method for Determination of Organophosphorus Insecticide Residues in Fatty Processed Foods by Gel Permeation Chromatography

1995 ◽  
Vol 78 (6) ◽  
pp. 1502-1512 ◽  
Author(s):  
Anna Sannino ◽  
Paola Mambriani ◽  
Mirella Bandini ◽  
Luciana Bolzoni
Keyword(s):  
Methylene Chloride ◽  
Organophosphorus Compounds ◽  
Gel Permeation Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Gas Liquid Chromatography ◽  
Processed Foods ◽  
Multiresidue Method ◽  
Photometric Detection ◽  
Gel Permeation

Abstract A multiresidue method for quantitative determination of 39 organophosphorus compounds (parent pesticides and their major metabolites) in 7 fatty processed foods is described. Samples are extracted with methylene chloride and cleaned up by automated gel permeation chromatography with a Biobeads SX3 column and a methylene–chloridecyclohexane (15 + 85) eluant. Organophosphorus compounds are quantitated by capillary gas-liquid chromatography with flame photometric detection using OV-1701 and DB-5 columns. Average recoveries from samples fortified at 0.025–1 mg/kg ranged from 50.6% for dichlorvos to 185% for malaoxon. Determination limits were between 0.005 and 0.040 μg/mL. Results were confirmed by gas chromatography/mass spectrometry with selected-ion monitoring.

Reliable Enzyme-Linked Immunosorbent Assay for the Determination of Soybean Proteins in Processed Foods

2008 ◽  
Vol 56 (16) ◽  
pp. 6818-6824 ◽  
Author(s):  
Naoki Morishita ◽  
Kumiko Kamiya ◽  
Takashi Matsumoto ◽  
Shinobu Sakai ◽  
Reiko Teshima ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Foods ◽  
Soybean Proteins
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