An immunological determination of somatostatin in pharmaceutical by sandwich ELISA based on IgY and polyclonal antibody

2019 ◽  
Vol 145 ◽  
pp. 532-538
Author(s):  
Yingshan Chen ◽  
Yuxin Liang ◽  
Rui Lv ◽  
Nana Xia ◽  
Tianjian Xue ◽  
...  
Keyword(s):  
Polyclonal Antibody ◽  
Sandwich Elisa

Lactate Dehydrogenase Polyclonal Antibody Sandwich ELISA for Determination of Endpoint Heating Temperatures of Ground Beef

1996 ◽  
Vol 59 (1) ◽  
pp. 51-55 ◽  
Author(s):  
CHENG-HSIN WANG ◽  
MOHAMED M. ABOUZIED ◽  
JAMES J. PESTKA ◽  
DENISE M. SMITH
Keyword(s):  
Lactate Dehydrogenase ◽  
Polyclonal Antibody ◽  
Heating Temperature ◽  
Polyclonal Antibodies ◽  
Sandwich Elisa ◽  
Ground Beef ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Marker ◽  
Beef Patties

An enzyme-linked immunosorbent assay (ELISA) for the protein marker lactate dehydrogenase (LDH) was developed to determine endpoint heating temperature (EPT) of ground beef. Ground beef was placed in 10 by 75 mm tubes and heated to temperatures between 62 and 74°C at 2°C intervals. Electrophoresis and immunoblotting of meat extracts indicated a decrease in the intensity of the LDH band as temperature was increased. Polyclonal antibodies (PAb) against bovine muscle LDH were produced and a sandwich ELISA devised using biotinylated PAb as the detecting antibody. The LDH content decreased (P < 0.05) in ground beef heated between 66 and 74°C and was less than 4 μg/g of meat at the recommended minimum endpoint of 69°C. The ELISA was used to estimate the EPT of commercially cooked beef patties.

scholarly journals Determination of Aspergillus pathogens in agricultural products by a specific nanobody-polyclonal antibody sandwich ELISA

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ting Wang ◽  
Peiwu Li ◽  
Qi Zhang ◽  
Wen Zhang ◽  
Zhaowei Zhang ◽  
...  
Keyword(s):  
Polyclonal Antibody ◽  
Sandwich Elisa ◽  
Agricultural Products

Determination of Cardiac Disease Biomarker by Plasmonic Sandwich‐ELISA

2020 ◽  
Author(s):  
Weirong Wei ◽  
Yinyan Tang ◽  
Huimin He ◽  
Subash C.B. Gopinath ◽  
Lingling Wang
Keyword(s):  
Cardiac Disease ◽  
Sandwich Elisa ◽  
Disease Biomarker

Development and Characterization of Anti-Estradiol Polyclonal Antibody

2012 ◽  
Vol 461 ◽  
pp. 67-70 ◽  
Author(s):  
Chao Ying Li ◽  
Jin Qing Jiang
Keyword(s):  
Polyclonal Antibody ◽  
Dynamic Range ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Uv Absorbance ◽  
Standard Curve ◽  
Ic50 Value ◽  
New Zealand White Rabbits

This paper reports an indirect competitive enzyme-linked immunosorbent assay (icELISA) using polyclonal antibody (pAb) for estradiol (E2) residues. After derivation, E2 haptens were conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) through 1-Ethyl-3-(3-dimethylaminopropy) carbodiimide (EDC) method, and New Zealand white rabbits were immunized to produce anti-E2 pAb. The conjugation ratio of E2-BSA was proved to be 18.6:1 by an UV absorbance method. Based on the square matrix titration, an icELISA standard curve was developed. The dynamic range was from 0.16 to 128 ng/mL, with LOD and IC50 value of 0.08 ng/mL and 3.76 ng/mL, respectively. Except for a little cross-reactivity (16.2%) to estrone, this assay showed negligible cross-reactivity to other analogues tested. The results suggest that the produced anti-E2 pAb could be used to develop an icELISA method for the determination of E2 residues in animal-originally products.

scholarly journals Use of a Polyclonal Antibody for the Determination of the Prognostic Value of c-erbB-2 Protein Over-Expression in Human Breast Cancer

1996 ◽  
Vol 35 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Philippe Terrier ◽  
Hélène Mouriesse ◽  
Brigitte Loridon ◽  
Marianne Gotteland ◽  
Françoise May-Levin ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Polyclonal Antibody ◽  
Human Breast Cancer ◽  
Prognostic Value ◽  
Human Breast ◽  
Over Expression

scholarly journals A Sandwich ELISA for the Determination of Beef Meat Content in Processed Foods

2009 ◽  
Vol 15 (6) ◽  
pp. 613-618 ◽  
Author(s):  
Satoshi KOTOURA ◽  
Yukie MURAKAMI-YAMAGUCHI ◽  
Miyuki NAKAMURA ◽  
Kiyotaka MIAKE ◽  
Masaaki SUGIYAMA ◽  
...  
Keyword(s):  
Sandwich Elisa ◽  
Processed Foods ◽  
Beef Meat ◽  
Meat Content

A novel method for artificial antigen synthesis and preparation of a polyclonal antibody for the sensitive determination of leucomalachite green in fish samples by enzyme-linked immunoassay

2016 ◽  
Vol 8 (32) ◽  
pp. 6236-6243 ◽  
Author(s):  
Dan Zhu ◽  
Yang Mu ◽  
Qiangqiang Li ◽  
Xiumei Pang ◽  
Xue Wang ◽  
...  
Keyword(s):  
Polyclonal Antibody ◽  
Detection Method ◽  
Artificial Antigen ◽  
Fish Samples ◽  
Leucomalachite Green ◽  
Novel Method ◽  
Sensitive Determination

A novel hapten for leucomalachite green was successfully synthesized, and the prepared polyclonal antibody was used to develop a sensitive ELISA detection method for leucomalachite green in fish.

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