Determination of Cardiac Disease Biomarker by Plasmonic Sandwich‐ELISA

2020 ◽  
Author(s):  
Weirong Wei ◽  
Yinyan Tang ◽  
Huimin He ◽  
Subash C.B. Gopinath ◽  
Lingling Wang
Keyword(s):  
Cardiac Disease ◽  
Sandwich Elisa ◽  
Disease Biomarker

An immunological determination of somatostatin in pharmaceutical by sandwich ELISA based on IgY and polyclonal antibody

2019 ◽  
Vol 145 ◽  
pp. 532-538
Author(s):  
Yingshan Chen ◽  
Yuxin Liang ◽  
Rui Lv ◽  
Nana Xia ◽  
Tianjian Xue ◽  
...  
Keyword(s):  
Polyclonal Antibody ◽  
Sandwich Elisa

scholarly journals A Sandwich ELISA for the Determination of Beef Meat Content in Processed Foods

2009 ◽  
Vol 15 (6) ◽  
pp. 613-618 ◽  
Author(s):  
Satoshi KOTOURA ◽  
Yukie MURAKAMI-YAMAGUCHI ◽  
Miyuki NAKAMURA ◽  
Kiyotaka MIAKE ◽  
Masaaki SUGIYAMA ◽  
...  
Keyword(s):  
Sandwich Elisa ◽  
Processed Foods ◽  
Beef Meat ◽  
Meat Content

QUANTITATIVE DOUBLE ANTIBODY SANDWICH ELISA FOR THE DETERMINATION OF GLIADIN

2012 ◽  
Vol 33 (4) ◽  
pp. 339-351 ◽  
Author(s):  
Naiyana Gujral ◽  
Mavanur R. Suresh ◽  
Hoon H. Sunwoo
Keyword(s):  
Sandwich Elisa ◽  
Double Antibody

Quantitative Sandwich ELISA for the Determination of Lupine (Lupinusspp.) in Foods

2005 ◽  
Vol 53 (15) ◽  
pp. 5866-5871 ◽  
Author(s):  
Lise Holden ◽  
Eliann Egaas
Keyword(s):  
Sandwich Elisa

New sandwich ELISA for human urinary N-acetyl-β-d-glucosaminidase isoenzyme B as a useful clinical test

1997 ◽  
Vol 43 (4) ◽  
pp. 569-574 ◽  
Author(s):  
Yoshito Numata ◽  
Atsushi Morita ◽  
Yoko Kosugi ◽  
Kazunori Shibata ◽  
Nozomu Takeuchi ◽  
...  
Keyword(s):  
Human Urine ◽  
Sandwich Elisa ◽  
Sample Volume ◽  
Mean Value ◽  
Cross Reactivity ◽  
Urine Samples ◽  
Clinical Test ◽  
The Mean ◽  
Dilution Curves

Abstract We have developed a new ELISA for quantifying N-acetyl-β-d-glucosaminidase (NAG) isoenzyme B in human urine after raising monoclonal antibodies against the isoenzyme from human placenta. Though the obtained antibodies reacted not only to isoenzyme B but also to A, we could detect isoenzyme B selectively by a two-step sandwich ELISA with a pair of selected antibodies at low pH in the first reaction. The detected limit was 0.5 μg/L for a sample volume of 25 μL. Within-run CVs ranged from 2.5% to 5.4% and between-run CVs ranged from 6.2% to 9.1%. Recoveries of NAG isoenzyme B added to each of three urine samples ranged from 91% to 114%. The dilution curves of urine samples showed good linearity. The cross-reactivity of NAG isoenzyme A was practically negligible (2–3%). The mean value for NAG isoenzyme B in spot urines from healthy adults was 2.9 μg/g creatinine. This ELISA method is rapid and precise enough for routine determination of NAG isoenzyme B in human urine.

Lactate Dehydrogenase Polyclonal Antibody Sandwich ELISA for Determination of Endpoint Heating Temperatures of Ground Beef

1996 ◽  
Vol 59 (1) ◽  
pp. 51-55 ◽  
Author(s):  
CHENG-HSIN WANG ◽  
MOHAMED M. ABOUZIED ◽  
JAMES J. PESTKA ◽  
DENISE M. SMITH
Keyword(s):  
Lactate Dehydrogenase ◽  
Polyclonal Antibody ◽  
Heating Temperature ◽  
Polyclonal Antibodies ◽  
Sandwich Elisa ◽  
Ground Beef ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Marker ◽  
Beef Patties

An enzyme-linked immunosorbent assay (ELISA) for the protein marker lactate dehydrogenase (LDH) was developed to determine endpoint heating temperature (EPT) of ground beef. Ground beef was placed in 10 by 75 mm tubes and heated to temperatures between 62 and 74°C at 2°C intervals. Electrophoresis and immunoblotting of meat extracts indicated a decrease in the intensity of the LDH band as temperature was increased. Polyclonal antibodies (PAb) against bovine muscle LDH were produced and a sandwich ELISA devised using biotinylated PAb as the detecting antibody. The LDH content decreased (P < 0.05) in ground beef heated between 66 and 74°C and was less than 4 μg/g of meat at the recommended minimum endpoint of 69°C. The ELISA was used to estimate the EPT of commercially cooked beef patties.

Development, Optimization and Validation of a Sandwich ELISA for Detection of Recombinant Human Factor VII

2015 ◽  
Vol 5 (1) ◽  
pp. 26-31
Author(s):  
Sheila Sarial ◽  
Rozhan Sonboli ◽  
Shayan Maleknia ◽  
Fatemeh Ashori ◽  
Sara Rezaeiravesh ◽  
...  
Keyword(s):  
Low Dose ◽  
Human Factor ◽  
Sandwich Elisa ◽  
Factor Vii ◽  
Enzyme Linked Immunosorbent Assay ◽  
P Value ◽  
Limit Of Quantification ◽  
Intermediate Precision ◽  
Downstream Processes

Recombinant human factor VII (rh-FVII) is produced by engineered BHK cells at low dose. Accordingly, establishment of a precise method is crucial to reliably measuring expression level of this protein during manufacturing pro-cesses. We developed and established a reproducible sandwich enzyme-linked immunosorbent assay (ELISA) method for measuring amount of FVII during biopharmaceutical in upstream and downstream processes of Aryo-Seven. A sandwich ELISA was designed using two different high affinity mon-oclonal antibodies (mAb1 and mAb2) against h-FVII. The bounded FVII to the first antibody was revealed by the use of a second mouse anti-FVII monoclo-nal antibody (1F1-B11), labeled with HRP that binds to another antigenic determinant of the FVII. Then, validation was done by determination of spec-ificity, linearity, accuracy, precision, reproducibility, detection and quantifica-tion limit and robustness according to ICH Q2 (R1) guideline. In developed ELISA, no interference was found between FVII and proteins derived from BHK which commonly exist in the supernatant. Linear range of detection was from 25-1.56 ng/mL with P-Value <0.001. In accuracy, spiked samples showed 109±2% recovery. Intra and intermediate precision assays showed %RSD not more than20. Detection limit of this assay was 0.99 ng /mL and limit of quantification was 2.99 ng/ml. The sandwich ELISA was found to be useful tool for measuring FVII/ FVIIa. The ELISA approach was precise, repro-ducible, and accurate. The ELISA therefore, is offered as an assured kit for detection of recombinant human factor.

scholarly journals An Improved Sandwich ELISA Method for the Determination of Immunoreactive Schizophyllan (SPG).

1994 ◽  
Vol 17 (11) ◽  
pp. 1437-1440 ◽  
Author(s):  
Akio HIRATA ◽  
Makiko KOMODA ◽  
Wataru ITOH ◽  
Kengo TABATA ◽  
Shinji ITOYAMA ◽  
...  
Keyword(s):  
Sandwich Elisa ◽  
Elisa Method
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