scholarly journals AN INDUCED-FLOW KIDNEY-BREAST CANCER IN VITRO MODEL FOR STUDY OF CANCER SUPPRESSION BY VITAMIN D3 DERIVATIVES

2014 ◽  
Author(s):  
S. Noh ◽  
P.K. Yuen ◽  
V.N. Goral ◽  
H. Kim
Keyword(s):  
Breast Cancer ◽  
Vitamin D3 ◽  
In Vitro Model ◽  
Induced Flow ◽  
Vitro Model ◽  
Cancer Suppression

scholarly journals Polyurethane foam scaffolds: a novel 3D in vitro model for breast cancer-derived bone metastasis

2016 ◽  
Vol 4 ◽  
Author(s):  
Contessi Nicola ◽  
Angeloni Valentina ◽  
De Marco Cinzia ◽  
Tanzi Maria Cristina ◽  
Daidone Maria Grazia ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Bone Metastasis ◽  
Polyurethane Foam ◽  
In Vitro Model ◽  
3D In Vitro Model ◽  
Vitro Model

In vitro study of placental trophoblast calcium uptake using JEG-3 human choriocarcinoma cells

1991 ◽  
Vol 98 (3) ◽  
pp. 333-342
Author(s):  
R.S. Tuan ◽  
C.J. Moore ◽  
J.W. Brittingham ◽  
J.J. Kirwin ◽  
R.E. Akins ◽  
...  
Keyword(s):  
Vitamin D3 ◽  
Calcium Uptake ◽  
In Vitro Model ◽  
Dependent Manner ◽  
Choriocarcinoma Cells ◽  
Vitro Model ◽  
Human Choriocarcinoma Cells ◽  
Functional Components ◽  
1,25 Dihydroxy Vitamin D3

During human fetal development, placental syncytiotrophoblasts actively transport calcium from the maternal to the fetal circulation. Two functional components, a cytosolic Ca2(+)-binding protein (CaBP) and a Ca2(+)-ATPase have been identified in the syncytiotrophoblasts of the chorionic villi. We report here the calcium uptake properties of a human choriocarcinoma cell line, JEG-3, which was used as an in vitro model cell system for the syncytiotrophoblasts. In culture, JEG-3 proliferated as large syncytial aggregates expressing typical syncytiotrophoblast markers. 45Ca uptake by JEG-3 was a substrate- and temperature-dependent, membrane-mediated active process that exhibited linear kinetics for up to 7 min. Both the CaBP and the Ca2(+)-ATPase were expressed by JEG-3, on the basis of biochemical, histochemical, immunochemical and or mRNA assays. Immunohistochemistry and in situ hybridization revealed that JEG-3 cells were heterogeneous with respect to the expression of the CaBP. The Ca2(+)-ATPase activity of JEG-3 was similar to the placental enzyme in terms of sensitivity to specific inhibitors, and was detected histochemically along the cell membrane. Fura-2 Ca2+ imaging revealed that calcium uptake by JEG-3 was not accompanied by a concomitant increase in cytosolic [Ca2+], suggesting a specific Ca2+ sequestration mechanism. The involvement of calciotropic hormonal regulation was evaluated by studying the response of JEG-3 to 1,25-dihydroxy vitamin D3. Calcium uptake was significantly stimulated in a dose-dependent manner by a 24-h treatment of the cells with 1,25-dihydroxy vitamin D3 (optimal dose approximately 0.5 nM); the CaBP level doubled whereas steady-state CaBP mRNA did not, suggesting that CaBP expression was regulated by 1,25-dihydroxy vitamin D3. These observations strongly suggest that the JEG-3 human choriocarcinoma cells should serve as a convenient in vitro model system for studying the cellular mechanism and regulation of transplacental calcium transport.

scholarly journals The Wnt signalling pathway is upregulated in an in vitro model of acquired tamoxifen resistant breast cancer

BMC Cancer ◽  
2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Yan Ni Loh ◽  
Ellen L Hedditch ◽  
Laura A Baker ◽  
Eve Jary ◽  
Robyn L Ward ◽  
...  
Keyword(s):  
Breast Cancer ◽  
In Vitro Model ◽  
Wnt Signalling ◽  
Signalling Pathway ◽  
Wnt Signalling Pathway ◽  
Tamoxifen Resistant ◽  
Vitro Model

scholarly journals A microfluidic 3D in vitro model for specificity of breast cancer metastasis to bone

Biomaterials ◽  
2014 ◽  
Vol 35 (8) ◽  
pp. 2454-2461 ◽  
Author(s):  
Simone Bersini ◽  
Jessie S. Jeon ◽  
Gabriele Dubini ◽  
Chiara Arrigoni ◽  
Seok Chung ◽  
...  
Keyword(s):  
Breast Cancer ◽  
In Vitro Model ◽  
Cancer Metastasis ◽  
Breast Cancer Metastasis ◽  
3D In Vitro Model ◽  
Vitro Model

In Vitro Model Systems for the Study of Hormone-Dependent Human Breast Cancer

1977 ◽  
Vol 296 (3) ◽  
pp. 154-159 ◽  
Author(s):  
Howard L. Bleich ◽  
Emily S. Boro ◽  
Marc E. Lippman ◽  
C. Kent Osborne ◽  
Richard Knazek ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
In Vitro Model ◽  
Model Systems ◽  
Human Breast ◽  
Vitro Model

scholarly journals Transition to invasion in breast cancer: a microfluidic in vitro model enables examination of spatial and temporal effects

2011 ◽  
Vol 3 (4) ◽  
pp. 439-450 ◽  
Author(s):  
Kyung Eun Sung ◽  
Ning Yang ◽  
Carolyn Pehlke ◽  
Patricia J. Keely ◽  
Kevin W. Eliceiri ◽  
...  
Keyword(s):  
Breast Cancer ◽  
In Vitro Model ◽  
Temporal Effects ◽  
Vitro Model

scholarly journals Correction: An In Vitro Model That Recapitulates the Epithelial to Mesenchymal Transition (EMT) in Human Breast Cancer

PLoS ONE ◽  
2011 ◽  
Vol 6 (4) ◽  
Author(s):  
Elad Katz ◽  
Sylvie Dubois-Marshall ◽  
Andrew H. Sims ◽  
Philippe Gautier ◽  
Helen Caldwell ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
In Vitro Model ◽  
Epithelial To Mesenchymal Transition ◽  
Human Breast ◽  
Mesenchymal Transition ◽  
Vitro Model
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