scholarly journals The level of vascular endothelial growth factor in peripheral blood of type 2 diabetic patients depending on kidney functional state

2019 ◽  
pp. 13-19
Author(s):  
I. Topchii ◽  
T. Shcherban ◽  
V. Galchinska ◽  
P. Semenovykh ◽  
K. Savicheva
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Filtration Rate ◽  
Diabetic Patients ◽  
Vascular Endothelial ◽  
Type 2 Diabetic Patients ◽  
Type 2 Dm ◽  
Endothelial Growth Factor ◽  
Vegf Level ◽  
Type 2 Diabetic

Abstract. The aim of the study was to assess a serum level of vascular endothelial growth factor (VEGF) in type 2 diabetic patients depending on kidney function. Methods: we examined 66 type 2 diabetic patients and 20 healthy control subjects. Depending on the presence of albuminuria and glomerular filtration rate (GFR) level, patients with diabetes mellitus (DM) were divided into the following groups: group I - patients with type 2 DM without signs of nephropathy – normal GFR without albuminuria (n = 26); group ІІ - patients with type 2 DM with normal GFR and albuminuria (n = 22); group III - patients with type 2 DM with reduced GFR and albuminuria (n = 18). ІV group - patients with acute coronary syndrome (n = 26). The concentration of the VEGF was determined by enzyme-linked immunosorbent assay. The glomerular filtration rate (GFR) was calculated using the CKD EPI formula (KDIGO 2012). Results: serum VEGF level in patients with diabetic nephropathy (DN) was significantly increased depending on kidney function. VEGF positively correlated with urine albumin and serum creatinine levels and the negative correlation with GFR was found. These findings prove the undeniable role of kidneys in the development of renocardial syndrome in patients with DN. VEGF levels positively correlated with glycosylated hemoglobin and total cholesterol, indicating an unconditional dependence of endothelial dysfunction on lipid and glucose metabolism disorders. Conclusions: Increased levels of VEGF in type 2 diabetic patients without clinical signs of kidney damage may indicate the development of subclinical renal dysfunction. Blood VEGF in patients with DN may be an integral marker of endothelial dysfunction and pathological angiogenesis. Our findings suggest that determination of serum VEGF level in patients with DN may have practical use as an early predictor of cardiac disruption, estimation of the prognosis, as well as improvement of cardionephroprotective strategy.

scholarly journals Decreased vascular endothelial growth factor response to acute hypoglycemia in type 2 diabetic patients with hypoglycemic coma

Cytokine ◽  
2012 ◽  
Vol 57 (3) ◽  
pp. 372-378 ◽  
Author(s):  
Francisco Arnalich ◽  
Maria Constanza Maldifassi ◽  
Gema Atienza ◽  
Enrique Ciria ◽  
Angustias Quesada ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Diabetic Patients ◽  
Vascular Endothelial ◽  
Type 2 Diabetic Patients ◽  
Hypoglycemic Coma ◽  
Acute Hypoglycemia ◽  
Endothelial Growth Factor ◽  
Type 2 Diabetic

Uncoupling of Vascular Endothelial Growth Factor (VEGF) and Inducible Nitric Oxide Synthase (iNOS) in Gingival Tissue of Type 2 Diabetic Patients

Inflammation ◽  
2015 ◽  
Vol 39 (2) ◽  
pp. 632-642 ◽  
Author(s):  
Guendalina Lucarini ◽  
Giacomo Tirabassi ◽  
Antonio Zizzi ◽  
Giancarlo Balercia ◽  
Alexia Quaranta ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Gingival Tissue ◽  
Diabetic Patients ◽  
Vascular Endothelial ◽  
Type 2 Diabetic Patients ◽  
Oxide Synthase ◽  
Endothelial Growth Factor ◽  
Type 2 Diabetic ◽  
Inducible Nitric Oxide

scholarly journals Quantitave and qualitative changes in vascular endothelial growth factor gene expression in glomeruli of patients with type 2 diabetes

2004 ◽  
pp. 799-807 ◽  
Author(s):  
E Bortoloso ◽  
D Del Prete ◽  
M Dalla Vestra ◽  
G Gambaro ◽  
A Saller ◽  
...  
Keyword(s):  
Structural Parameters ◽  
Diabetic Patients ◽  
Mrna Levels ◽  
Vascular Endothelial ◽  
Vegf Mrna ◽  
Type 2 Diabetic Patients ◽  
Endothelial Growth Factor ◽  
Type 2 Diabetic ◽  
Qualitative Changes

OBJECTIVE: Vascular endothelial growth factor (VEGF) exists in three main splice variants, characterized by 121, 165 and 189 amino acids (VEGF 121, VEGF 165 and VEGF 189) and acts via two specific receptors: VEGF-R1 or Flt-1 and VEGF-R2 or KDR. VEGF plays an important role in the pathogenesis of diabetic retinopathy. This study examined the relationship between VEGF and its isoforms and the severity of diabetic nephropathy in type 2 diabetes. DESIGN: We evaluated the glomerular gene expression of VEGF and its receptors and studied the relationships with renal functional and structural parameters in type 2 diabetic patients. METHODS: Glomeruli from 17 kidney biopsies were microdissected; 14 out of 17 biopsies were also subjected to electron microscopic morphometric analysis to estimate glomerular structural parameters. VEGF mRNA was studied by comparative kinetic RT-PCR and real-time RT-PCR in order to identify the three different isoforms and to quantify VEGF, VEGF-R1 and VEGF-R2 mRNA levels. RESULTS: (i) Glomerular VEGF mRNA levels were inversely related to albumin excretion rate (r=-0.66, P=0.004); (ii) both the degree of mesangial and mesangial matrix expansion were inversely related to VEGF 165 mRNA levels (r=-0.73, P=0.005 and r=-0.64, P=0.017), and directly to VEGF 121 mRNA levels (r=0.74, P=0.003 and r=0.73, P=0.004); and (iii) VEGF and VEGF-R2 mRNA levels were directly related (r=0.62, P=0.033). CONCLUSIONS: These findings suggested that quantitative and qualitative changes in VEGF expression are present in type 2 diabetic patients with nephropathy and might be involved in the pathogenesis and progression of diabetic glomerulopathy.

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