scholarly journals A Preliminary Classification of Selected White Testa Peanuts (Arachis hypogaea L.) by Flavonoid Analysis

1983 ◽  
Vol 10 (1) ◽  
pp. 40-43 ◽  
Author(s):  
D. J. Daigle ◽  
Edith J. Conkerton ◽  
Ray O. Hammons ◽  
W. D. Branch
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Arachis Hypogaea ◽  
Aqueous Methanol ◽  
Arachis Hypogaea L ◽  
Sugar Derivatives ◽  
Derivatives Of ◽  
Preliminary Classification ◽  
Flavonoid Analysis

Abstract The testa and defatted flours of 32 white testa and the krinkleleaf tan spanish peanut (Arachis hypogaea L.) genotypes were analyzed for flavonoids in order to classify these peanuts for breeding purposes. Flavonoids were extracted from the testa and flours with aqueous methanol. These extracts were analyzed for flavonoids by high pressure liquid chromatography. UV spectrometry was used to identify the compounds. These flavonoids were principally sugar derivatives of the aglycones quercetin, rhamnetin, and isorhamnetin. Two other aglycones were detected and tentatively identified as isoflavones. From these data, it was possible to classify the 32 white testa and the krinkleleaf genotypes into several groups based upon the presence, absence, or ratio of various flavonoid compounds.

The Presence of 5,7-Dihydroxyisoflavone in Peanuts by High Performance Liquid Chromatography Analyses

1985 ◽  
Vol 12 (2) ◽  
pp. 60-62 ◽  
Author(s):  
D. J Daigle ◽  
R. L Ory ◽  
W. D Branch
Keyword(s):  
High Pressure ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Arachis Hypogaea ◽  
High Performance ◽  
Aqueous Methanol ◽  
Uv Spectrum ◽  
Tentative Identification ◽  
Arachis Hypogaea L ◽  
Flavonoid Aglycone

Abstract The defatted flours of 43 colored and one white testa peanut (Arachis hypogaea L.) genotypes were analyzed for flavonoids. Flavonoids were extracted from the flours with aqueous methanol, hydrolyzed, and the resulting aglycones analyzed by high pressure liquid chromatography with a variable wavelenth detector. The UV spectrum and retention time coupled with those of standards allowed for the tentative identification of the principal flavonoid aglycone as 5,7-dihydroxyisoflavone (DHI).

Extraction, Cleanup, and Quantitative Determination of Aflatoxins in Corn

1980 ◽  
Vol 63 (3) ◽  
pp. 631-633 ◽  
Author(s):  
James E Thean ◽  
David R Lorenz ◽  
David M Wilson ◽  
Kathleen Rodgers ◽  
Richard C Gueldner
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Ammonium Sulfate ◽  
Silica Gel ◽  
Normal Phase ◽  
Aqueous Methanol ◽  
Normal Phase Hplc ◽  
Water Saturated

Abstract A method is proposed for extraction and cleanup of corn samples for the quantitation of 4 aflatoxins by high pressure liquid chromatography (HPLC). After aqueous methanol extraction, ammonium sulfate treatment, and partition of aflatoxins into chloroform, sample extracts are partially purified on Sep-Pak cartridges or small columns packed with HPLC grade silica; cleanup requires only 13 mL solvent/sample. Aflatoxins B1, B2, G1, and G2 in the purified extract are resolved in ca 10 min by normal phase HPLC on a microparticulate (5 μm) silica gel column with a 50% water-saturated chloroform-cyclohexaneacetonitrile- ethanol solvent, and are measured by ultraviolet fluorescence in a silica gel-packed flowcell. Recoveries of added aflatoxins B1, B2, G1, and G2 were 84–118 % at levels of 1.5–125 μg/kg

Enhanced Detectability and Chromatography of Some Amines by High Pressure Liquid Chromatography

1980 ◽  
Vol 63 (4) ◽  
pp. 702-706
Author(s):  
F Taylor Noggle
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Secondary Amines ◽  
High Pressure Liquid Chromatographic ◽  
Ultraviolet Detection ◽  
Primary And Secondary Amines ◽  
Liquid Chromatographic ◽  
Derivatives Of

Abstract The high pressure liquid chromatographic properties of 13 phenylisothiocyanate derivatives of primary and secondary amines were examined with ultraviolet detection at 254 nm. Urine extracts of subjects who were taking ephedrine, phenylpropanolamine, and phentermine were also examined. The method described improves the chromatographic properties of the amines and also enhances their detectability.

2-O-Methyl-D-mannose, a key sugar in the taxonomy of Frankia

1983 ◽  
Vol 29 (8) ◽  
pp. 993-1002 ◽  
Author(s):  
A. Mort ◽  
P. Normand ◽  
M. Lalonde
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Host Plants ◽  
Major Product ◽  
Mass Spectrometric ◽  
Gas Liquid Chromatography ◽  
Methyl Glycoside ◽  
Biochemical Tests ◽  
Alditol Acetate

The classification of actinomycetes in the genus Frankia Brunchorst has been based partly on morphology but primarily on their ability to form N2-fixing nodules on roots of actinorhizal host plants. To identify nonsporulating and noninfective Frankia isolates, biochemical tests are needed. Sugars present in whole-cell hydrolysates have proven useful in the taxonomy of other genera of actinomycetes. We, and others, have found a sugar in all Frankia isolates examined thus far that is absent in all other actinomycetes tested. After reduction with NaBD2H4 and acetylation, the characteristic sugar was found by gas chromatographic and mass spectrometric analyses to be a 2-O-methyl hexose. The sugar was partially purified by high pressure liquid chromatography and demethylated to give a major product of mannose. Authentic 2-O-methyl mannose as its alditol acetate and trimethylsilyl methyl glycoside derivatives cochromatographed with the sugar. By gas–liquid chromatography of its trimethylsilyl (−)-2-butyl glycoside, the marker sugar was found to be 2-O-methyl-D-mannose. The 2-O-methyl-D-mannose was present in all 68 strains of Frankia isolated from the actinorhizae of seven species of Alnus, two species of Elaeagnus, and one species each of Myrica, Comptonia, and Shepherdia. These actinorhizal host plants originated from different provenances in Canada, U.S.A., France, and Holland.

Analysis of derivatives of carbohydrates by high-pressure liquid chromatography

1979 ◽  
Vol 76 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Charles A. White ◽  
John F. Kennedy ◽  
Bernard T. Golding
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Derivatives Of
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