The Presence of 5,7-Dihydroxyisoflavone in Peanuts by High Performance Liquid Chromatography Analyses

1985 ◽  
Vol 12 (2) ◽  
pp. 60-62 ◽  
Author(s):  
D. J Daigle ◽  
R. L Ory ◽  
W. D Branch
Keyword(s):  
High Pressure ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Arachis Hypogaea ◽  
High Performance ◽  
Aqueous Methanol ◽  
Uv Spectrum ◽  
Tentative Identification ◽  
Arachis Hypogaea L ◽  
Flavonoid Aglycone

Abstract The defatted flours of 43 colored and one white testa peanut (Arachis hypogaea L.) genotypes were analyzed for flavonoids. Flavonoids were extracted from the flours with aqueous methanol, hydrolyzed, and the resulting aglycones analyzed by high pressure liquid chromatography with a variable wavelenth detector. The UV spectrum and retention time coupled with those of standards allowed for the tentative identification of the principal flavonoid aglycone as 5,7-dihydroxyisoflavone (DHI).

scholarly journals A Preliminary Classification of Selected White Testa Peanuts (Arachis hypogaea L.) by Flavonoid Analysis

1983 ◽  
Vol 10 (1) ◽  
pp. 40-43 ◽  
Author(s):  
D. J. Daigle ◽  
Edith J. Conkerton ◽  
Ray O. Hammons ◽  
W. D. Branch
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Arachis Hypogaea ◽  
Aqueous Methanol ◽  
Arachis Hypogaea L ◽  
Sugar Derivatives ◽  
Derivatives Of ◽  
Preliminary Classification ◽  
Flavonoid Analysis

Abstract The testa and defatted flours of 32 white testa and the krinkleleaf tan spanish peanut (Arachis hypogaea L.) genotypes were analyzed for flavonoids in order to classify these peanuts for breeding purposes. Flavonoids were extracted from the testa and flours with aqueous methanol. These extracts were analyzed for flavonoids by high pressure liquid chromatography. UV spectrometry was used to identify the compounds. These flavonoids were principally sugar derivatives of the aglycones quercetin, rhamnetin, and isorhamnetin. Two other aglycones were detected and tentatively identified as isoflavones. From these data, it was possible to classify the 32 white testa and the krinkleleaf genotypes into several groups based upon the presence, absence, or ratio of various flavonoid compounds.

PREPARATION OF C60 BY DETONATION A MIXTURE OF TRINITROTOLUENE AND GRAPHITE

2012 ◽  
Vol 27 (01) ◽  
pp. 1350004
Author(s):  
XIANFENG WEI ◽  
YONG HAN ◽  
LIU LIU ◽  
XINPING LONG
Keyword(s):  
High Pressure ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Temperature ◽  
High Performance ◽  
Extreme Conditions ◽  
Detonation Pressure ◽  
Detonation Synthesis ◽  
Mass Ratio ◽  
Detonation Products

To explore the practicability of C 60 synthesis under extreme conditions (high pressure and high temperature), trinitrotoluene (TNT), trinitramine (RDX) and graphite mixtures of different proportions were detonated in a vacuum container, and the detonation products were collected for detecting. The results of mass spectroscopy, high performance liquid chromatography showed significant signals of C 60, which proved that C 60 could be synthesized by detonating the mixture of TNT and graphite (in 6:4 and 7:3 mass ratio, respectively), the detonation pressure and temperature were calculated around 13 GPa and 2000 K, respectively. Both experiment results and theoretical analysis showed the importance of detonation pressure and cooling temperature in detonation synthesis of C 60.

scholarly journals High-Pressure Open-Channel On-Chip Electroosmotic Pump for Nanoflow High Performance Liquid Chromatography

2014 ◽  
Vol 86 (4) ◽  
pp. 1958-1964 ◽  
Author(s):  
Wei Wang ◽  
Congying Gu ◽  
Kyle B. Lynch ◽  
Joann J. Lu ◽  
Zhengyu Zhang ◽  
...  
Keyword(s):  
High Pressure ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Open Channel ◽  
Electroosmotic Pump ◽  
On Chip

Rapid estimation of corynetoxins in bacterial galls from annual ryegrass (Lolium rigidum Gaudin) by high-performance liquid chromatography

1985 ◽  
Vol 36 (1) ◽  
pp. 35 ◽  
Author(s):  
PA Cockrum ◽  
JA Edgar
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
South Australia ◽  
Reversed Phase ◽  
Annual Ryegrass ◽  
Lolium Rigidum ◽  
Aqueous Methanol ◽  
The Individual ◽  
Annual Ryegrass Toxicity

A rapid and sensitive method is described for the extraction, detection and measurement of corynetoxins in ryegrass seed galls colonized by Corynebucterium rathayi. Excised galls are extracted with aqueous methanol and the extract analysed, without further purification, by reversed-phase high-performance liquid chromatography with detection by uItraviolet absorption. The method is applicable down to the level of a single gall or part of a gall with a minimum level of detection of about 0.25 �g using the equipment described. The corynetoxin content of the individual galls examined varied from below the detection limit to 10.6 �g. The highest level of corynetoxin in a single gall was 1.15% of gall weight. The corynetoxin composition of galls collected during outbreaks of annual ryegrass toxicity in South Australia, Western Australia and South Africa during a 19-year period were found to be similar.

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