scholarly journals Global Gene Expression Profiling of Body-Mass Index in Middle-Aged Danish Twins

2020 ◽  
pp. 1-8
Author(s):  
Qihua Tan ◽  
Weilong Li ◽  
Jan Baumbach ◽  
Afsaneh Mohammadnejad ◽  
Jesper Lund ◽  
...  
Keyword(s):  
Gene Expression ◽  
Body Mass Index ◽  
Gene Expression Profiling ◽  
Body Mass ◽  
Expression Profiling ◽  
Monozygotic Twins ◽  
Global Gene Expression ◽  
The Body ◽  
Obesity And Diabetes ◽  
Global Gene Expression Profiling

Objective: The body mass index (BMI) measured as weight in relation to height is an important monitor for obesity and diabetes, with individual variation under control by genetic and environmental factors. In transcriptome-wide association studies on BMI, the genetic contribution calls for controlling of genetic confounding that affects both BMI and gene expression. We performed a global gene expression profiling of BMI on peripheral blood cells using monozygotic twins for efficient handling of genetic make-ups. Methods: We applied a generalized association method to genome-wide gene expression data on 229 pairs of monozygotic twins (age 56-80 years) for detecting diverse patterns of correlation between BMI and gene expression. Results: We detected seven probes associated with BMI with p<1e-04, among them two probes with p<1e05 (p=2.83e-06 AAK1; p=7.83e-06 LILRA3). In total, the analysis found 1579 probes with nominal p<0.05. Biological pathway analysis of enriched pathways found 50 KEGG and 45 Reactome pathways (FDR<0.05). The identified top functional pathways included immune function, JAK-STAT signalling, insulin signalling and regulation of energy metabolism. Conclusion: This transcriptome-wide association study using monozygotic twins and generalized correlation identified differentially expressed genes and a broad spectrum of enriched biological pathways that may implicate metabolic health.

Global gene expression profiling of porcine endometria on Days 12 and 16 of the estrous cycle and pregnancy

2014 ◽  
Vol 82 (6) ◽  
pp. 897-909 ◽  
Author(s):  
Jolanta Kiewisz ◽  
Kamil Krawczynski ◽  
Pawel Lisowski ◽  
Agnieszka Blitek ◽  
Lech Zwierzchowski ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Estrous Cycle ◽  
Expression Profiling ◽  
Global Gene Expression ◽  
Global Gene Expression Profiling

scholarly journals Global Gene Expression Profiling of Circulating Tumor Cells

2005 ◽  
Vol 65 (12) ◽  
pp. 4993-4997 ◽  
Author(s):  
Denis A. Smirnov ◽  
Daniel R. Zweitzig ◽  
Bradley W. Foulk ◽  
M. Craig Miller ◽  
Gerald V. Doyle ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Expression Profiling ◽  
Global Gene Expression ◽  
Global Gene Expression Profiling

Global gene expression profiling in early-stage polycystic kidney disease in the Han:SPRD Cy rat identifies a role for RXR signaling

2011 ◽  
Vol 300 (1) ◽  
pp. F177-F188 ◽  
Author(s):  
Masanori Kugita ◽  
Kazuhiro Nishii ◽  
Miwa Morita ◽  
Daisuke Yoshihara ◽  
Hiroe Kowa-Sugiyama ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Kidney Disease ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Early Stage ◽  
Global Gene Expression ◽  
Rt Pcr ◽  
Polycystic Kidney ◽  
Global Gene Expression Profiling

Han:SPRD Cy is a spontaneous rat model of polycystic kidney disease (PKD) caused by a missense mutation in Pkdr1. Cystogenesis in this model is not clearly understood. In the current study, we performed global gene expression profiling in early-stage PKD cyst development in Cy/Cy kidneys and normal (+/+) kidneys at 3 and 7 days of postnatal age. Expression profiles were determined by microarray analysis, followed by validation with real-time RT-PCR. Genes were selected with over 1.5-fold expression changes compared with age-matched +/+ kidneys for canonical pathway analysis. We found nine pathways in common between 3- and 7-day Cy/Cy kidneys. Three significantly changed pathways were designated “Vitamin D Receptor (VDR)/Retinoid X Receptor (RXR) Activation,” “LPS/IL-1-Mediated Inhibition of RXR Function,” and “Liver X Receptor (LXR)/RXR Activation.” These results suggest that RXR-mediated signaling is significantly altered in developing kidneys with mutated Pkdr1. In gene ontology analysis, the functions of these RXR-related genes were found to be involved in regulating cell proliferation and organ morphogenesis. With real-time RT-PCR analysis, the upregulation of Ptx2, Alox15b, OSP, and PCNA, major markers of cell proliferation associated with the RXR pathway, were confirmed in 3- and 7-day Cy/Cy kidneys compared with 3-day +/+ kidneys. The increased RXR protein was observed in both the nucleus and cytoplasm of cystic epithelial cells in early-stage Cy/Cy kidneys, and the RXR-positive cells were strongly positive for PCNA staining. Taken together, cell proliferation and organ morphogenesis signals transduced by RXR-mediated pathways may have important roles for cystogenesis in early-stage PKD in this Pkdr1-mutated Cy rat.

Sign in / Sign up

Export Citation Format

Share Document