scholarly journals Capnocytophaga canimorsus Meningitis – Diagnosis with 16S rDNA PCR when Conventional Methods Failed to Identify the Causative Agent

2021 ◽  
Vol 3 (4) ◽  
pp. 01-06
Author(s):  
Anurup Kumar
Keyword(s):  
Causative Agent ◽  
High Morbidity ◽  
Normal Flora ◽  
Chain Reaction ◽  
Capnocytophaga Canimorsus ◽  
Neurological Sequelae ◽  
16S Ribosomal Dna ◽  
16S Rdna Pcr ◽  
Polymerase Chain ◽  
Conventional Methods

Meningitis whether bacterial or viral, poses many challenges to clinicians as the causative agent is often not found. According to guidelines, it is standard to start empiric treatment before a Cerebrospinal Fluid (CSF) sample is obtained. Meningitis, if not diagnosed and treated early, can lead to high morbidity and mortality rates with serious neurological sequelae. While the most common cases of bacterial meningitis are related to Streptococcus pneumoniae and Neisseria meningitidis, this clinical case report found a rare case of meningitis caused by a zoonotic pathogen, Capnocytophaga canimorsus; a commensal found as part of the normal flora of dogs and cats. This rare organism was identified with the help of broad range 16S ribosomal DNA Polymerase Chain Reaction (rDNA PCR), an emerging technique that is now increasingly useful in rapid diagnosis especially if the offending agent is not timely identified and conventional methods have failed, making diagnosis and management difficult for physicians.

scholarly journals Molecular Characterization and Classification of Phytoplasmas Associated with Canola Yellows and a New Phytoplasma Strain Associated with Dandelions

Plant Disease ◽  
2001 ◽  
Vol 85 (1) ◽  
pp. 76-79 ◽  
Author(s):  
Keri Wang ◽  
Chuji Hiruki
Keyword(s):  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
Universal Primers ◽  
Dna Fragments ◽  
Chain Reaction ◽  
Aster Yellows ◽  
Distinct Subgroup ◽  
16S Ribosomal Dna ◽  
Polymerase Chain

DNA isolated from symptomatic canola (Brassica napus, Brassica rapa) and dandelion (Taraxacum officinale) was used to amplify 16S ribosomal DNA fragments by polymerase chain reaction using two pairs of universal primers P1/P6 and R16F2n/R2. Restriction fragment length polymorphism (RFLP) analysis of the amplified DNA fragments using endonucleases AluI, HhaI, HpaII, MseI, RsaI, and Sau 3AI revealed two distinct types of phytoplasmas in canola with similar symptoms. One had the same RFLP profiles as the phytoplasmas in subgroup 16SrI-A, whereas the other one had RFLP profiles similar to those of phytoplasmas in subgroup 16SrI-B. Phytoplasmas were detected in symptomatic dandelion plants that were collected from canola and alfalfa fields where severe alfalfa witches'-broom occurred. Comparative studies indicated that two different phytoplasmas were associated with the dandelion plants. One was identified as a member of subgroup 16SrI-A, whereas another one was classified as a member of a distinct subgroup in the aster yellows group on the basis of the unique RFLP patterns.

Polymerase chain reaction-mediated genotyping of Hortaea werneckii, causative agent of tinea nigra

Mycoses ◽  
1994 ◽  
Vol 37 (9-10) ◽  
pp. 307-312 ◽  
Author(s):  
J. M. J. Uijthof ◽  
A. W. A. M. de Cock ◽  
G. S. de Hoog ◽  
W. G. V. Quint ◽  
A. van Belkum
Keyword(s):  
Polymerase Chain Reaction ◽  
Causative Agent ◽  
Chain Reaction ◽  
Hortaea Werneckii ◽  
Tinea Nigra ◽  
Polymerase Chain

scholarly journals Meningitis with polymerase chain reaction for varicella zoster positivity in cerebrospinal flid of a young immunocompetent adult

2016 ◽  
Vol 7 (04) ◽  
pp. 591-593 ◽  
Author(s):  
Pooja Gupta ◽  
Rajeev Ranjan ◽  
C. S. Agrawal ◽  
K Muralikrishnan ◽  
Nikhil Dave ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Healthy Male ◽  
Chain Reaction ◽  
Immunocompetent Adult ◽  
Neurological Sequelae ◽  
Varicella Zoster ◽  
Complete Physical Examination ◽  
Polymerase Chain ◽  
High Index Of Suspicion ◽  
Immunocompetent Adults

ABSTRACTMeningitis caused by varicella zoster virus (VZV) is quite rare among young immunocompetent adults though immunocompromised patients are often seen to be affected by reactivation of VZV presenting with primary clinical features of dermatomal rashes and neurological sequelae. Here, we report the clinical scenario of a young, healthy male who had presented with fever, headache, and onset of dermatomal rashes later than the fever and was eventually diagnosed to be a case of VZV meningitis. We would like to highlight the fact that even young immunocompetent patients though rarely, might contract VZV meningitis and clinicians should have a high index of suspicion and keen eyes to catch the more obvious features of VZV infection on complete physical examination and must not harbor any reservations in ordering polymerase chain reaction for VZV DNA or initiating aggressive antiviral therapy.

Sign in / Sign up

Export Citation Format

Share Document