MOLECULAR-GENETIC CRITERIA AND PROSPECTIVE BIOMARKERS OF SERRATED ADENOMAS OF COLORECTAL LOCALIZATION

A retrospective analysis of the database of patients with histologically verified serrated adenomas of the colon was carried out as part of an observational study. Inclusion criteria: patients with serrated colon adenomas who underwent a molecular genetic study to detect mutations and further sequencing 16 sRNAs of intestinal microbiota from January 2021 to October 2021. The exclusion criteria: patients with a primary tumor of the colon who had previously received complex treatment, who had accompanying pathologies of the other organs and systems, which did not allow for the planned examination. According to the results of the study, BRAF mutations are diagnosed in half of the cases in serrated adenomas and in one-third of cases - KRAS mutations. As a result of a comparative analysis of the intestinal microbiome of young patients with serrated colon adenomas with summary statistical data on the presence of bacteria in the intestines of people from the Russian population; promising markers were found for the development of effective approaches for the early diagnosis of colorectal cancer.

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MOLECULAR GENETIC STUDY OF SPECIES DIVERSITY AND QUANTITATIVE ANALYSIS OF THE INTESTINAL MICROBIOME OF APIS MELLIFERA L

10.51759/pchel_api_2021_52 ◽

2021 ◽

Author(s):

D.V. Boguslavsky ◽

E.I. Kovaleva

Keyword(s):

Quantitative Analysis ◽

Apis Mellifera ◽

Species Diversity ◽

Genetic Study ◽

Molecular Genetic ◽

Intestinal Microbiome ◽

Molecular Genetic Study

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Traditional serrated adenomas (TSAs) admixed with other serrated (so-called precursor) polyps and conventional adenomas: a frequent occurrence

Journal of Clinical Pathology ◽

10.1136/jclinpath-2014-202827 ◽

2015 ◽

Vol 68 (4) ◽

pp. 270-273 ◽

Cited By ~ 28

Author(s):

Runjan Chetty ◽

Sara Hafezi-Bakhtiari ◽

Stefano Serra ◽

Richard Colling ◽

Lai Mun Wang

Keyword(s):

Adenomatous Polyps ◽

Low Grade ◽

Precursor Lesion ◽

Braf Mutations ◽

Serrated Adenoma ◽

Kras Mutations ◽

Characteristic Type ◽

Serrated Polyp ◽

The Right ◽

Serrated Adenomas

BackgroundTraditional serrated adenoma (TSA) is a very characteristic type of serrated polyp that has a predilection for the left colon. Recent molecular advances have shown two molecular phenotypes of TSA: one associated with BRAF mutations and the other with KRAS mutations. The former is associated with hyperplastic polyps (HPs) and sessile serrated adenomas (SSAs), while the latter is associated with more conventional adenomatous dysplasia.AimsThe association of TSAs with so-called precursor lesions (HPs and SSAs) is not well recognised and the purpose of this study was to explore the coexistent presence of HPs, SSAs and adenomatous polyps within a large cohort of TSAs.MethodsIn total 149 TSAs were examined for the presence of HP, SSA and adenomatous polyps.ResultsSeen in 83 men and 65 women ranging in age from 32 to 89 years and 127 were left sided with 22 in the right colon. Seventy-eight of the 149 TSAs showed evidence of another polyp (52.34%): 32 were low-grade tubular/tubulovillous adenomas (TAs/TVAs; 41%), 28 were HPs (36%) and 18 were SSAs (23%). Eleven of the 22 right-sided TSAs were associated with a precursor lesion (1 HP and 7 SSA). In addition, five TSAs showed more than one polyp type: TSA with TA/TVA and HP (3); TSA with TA/TVA and SSA (2). The TAs/TVAs were adjacent to the TSA but occurred as a separate discrete polyp, while HPs and SSAs were intermingled with the TSA and present at the base and surface of the lesion.ConclusionsMore than 50% of TSAs are associated with a precursor lesion or adjacent TA/TVA. Their recognition is important as this may have surveillance and management ramifications.

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No KRAS mutations found in gastrointestinal stromal tumors (GISTs): molecular genetic study of 514 cases

Modern Pathology ◽

10.1038/modpathol.2013.89 ◽

2013 ◽

Vol 26 (11) ◽

pp. 1488-1491 ◽

Cited By ~ 15

Author(s):

Jerzy Lasota ◽

Liqiang Xi ◽

Tiffany Coates ◽

RaShonda Dennis ◽

Moses O Evbuomwan ◽

...

Keyword(s):

Genetic Study ◽

Gastrointestinal Stromal Tumors ◽

Molecular Genetic ◽

Molecular Genetic Study ◽

Kras Mutations ◽

Stromal Tumors

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MicroRNA expression in SMARCB1/INI1-deficient sinonasal carcinoma: A clinicopathological, immunohistochemical and molecular genetic study

10.26226/morressier.596dfd59d462b80292387be2 ◽

2017 ◽

Author(s):

Jan Laco

Keyword(s):

Genetic Study ◽

Molecular Genetic ◽

Microrna Expression ◽

Molecular Genetic Study ◽

Sinonasal Carcinoma

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Challenges in the differential diagnosis of multiple endocrine neoplasia syndrome type 1 with isolated family hyperparathyroidism

Clinical Medicine (Russian Journal) ◽

10.30629/0023-2149-2020-98-3-218-225 ◽

2020 ◽

Vol 98 (3) ◽

pp. 218-225

Author(s):

J. A. Krupinova ◽

N. G. Mokrysheva ◽

N. Y. Kalinchenko ◽

A. K. Eremkina ◽

A. N. Polyakov ◽

...

Keyword(s):

Multiple Endocrine Neoplasia ◽

Molecular Genetic ◽

Pancreatic Neuroendocrine Tumor ◽

Family Type ◽

Dynamic Monitoring ◽

Heterozygous Mutation ◽

Molecular Genetic Study ◽

Men 1 ◽

Endocrine Neoplasia

Multiple endocrine neoplasia type 1 (MEN-1) is the most common cause of the hereditary type of primary hyperparathyroidism (PHPT). If a family type of PHPT is suspected, a dynamic monitoring of patients and their close relatives should be carried out throughout their lives. We present a clinical case of a family in which four members of a pedigree were diagnosed with familial isolated hyperparathyroidism (FIHP). The diagnosis was changed to MEN-1, because it appeared that one of the patients had pancreatic neuroendocrine tumor. Molecular genetic study of MEN1 by direct by means of Sanger sequencing revealed that six family members had a new heterozygous mutation in exon 9: s. 1252 G> T p. D418Y.

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