Protective effect of high-affinity liposomes encapsulating astaxanthin against corneal disorder in the <i>in vivo</i> rat dry eye disease model

Purpose. To evaluate the efficacy of self-retained cryopreserved amniotic membrane (CAM) in promoting corneal nerve regeneration and improving corneal sensitivity in dry eye disease (DED).Methods. In this prospective randomized clinical trial, subjects with DED were randomized to receive CAM (study group) or conventional maximum treatment (control). Changes in signs and symptoms, corneal sensitivity, topography, and in vivo confocal microscopy (IVCM) were evaluated at baseline, 1 month, and 3 months.Results. Twenty subjects (age 66.9 ± 8.9) were enrolled and 17 completed all follow-up visits. Signs and symptoms were significantly improved in the study group yet remained constant in the control. IVCM showed a significant increase in corneal nerve density in the study group (12,241 ± 5083 μm/mm2at baseline, 16,364 ± 3734 μm/mm2at 1 month, and 18,827 ± 5453 μm/mm2at 3 months,p=0.015) but was unchanged in the control. This improvement was accompanied with a significant increase in corneal sensitivity (3.25 ± 0.6 cm at baseline, 5.2 ± 0.5 cm at 1 month, and 5.6 ± 0.4 cm at 3 months,p<0.001) and corneal topography only in the study group.Conclusions. Self-retained CAM is a promising therapy for corneal nerve regeneration and accelerated recovery of the ocular surface health in patients with DED. The study is registered at clinicaltrials.gov with trial identifier:NCT02764814.

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A New Non-Human Primate Model of Desiccating Stress-Induced Dry Eye Disease

10.21203/rs.3.rs-1079358/v1 ◽

2021 ◽

Author(s):

Li Gong ◽

Yilin Guan ◽

WonKyung Cho ◽

Baowen Li ◽

Lingzhen Pan ◽

...

Keyword(s):

Dry Eye ◽

Clinical Symptoms ◽

Disease Model ◽

Tear Film ◽

Corticosteroid Treatment ◽

Dry Eye Disease ◽

Eye Disease ◽

Primate Model ◽

Desiccating Stress ◽

Human Primate

Abstract Dry eye disease (DED), a multifactorial ocular surface disease, is estimated to affect up to 34% of individuals over 50 years old. Although numerous animal models, including rodents and rabbits, have been developed to mimic the pathophysiologic mechanisms involved in the dry eye, there is a lack of non-human primate (NHP) models, critical for translational drug studies. Here, we developed a novel desiccating stress-induced dry eye disease model using rhesus monkeys. The monkeys were housed in a controlled-environment room for 21 to 36 days under humidity, temperature, and airflow regulation. Following desiccating stress, NHPs demonstrated clinical symptoms similar to those of humans, as shown by increased corneal fluorescein staining (CFS) and decreased tear-film breakup. Moreover, corticosteroid treatment significantly reduced CFS scoring, restored TFBUT, and prevented upregulation of tear proinflammatory cytokines as observed in dry eye patients following steroid treatment. These close resemblance of clinical symptoms and treatment responses to those of human DED patients provides great translational value to the NHP model which could serve as a clinically relevant animal model to study the efficacy of the new potential treatment for DED.

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A new approach of ocular nebulization with vitamin B12 versus oxytocin for the treatment of dry eye disease: an in vivo confocal microscopy study

Drug Design Development and Therapy ◽

10.2147/dddt.s203464 ◽

2019 ◽

Vol Volume 13 ◽

pp. 2381-2391

Author(s):

Jiarui Yang ◽

Yushi Liu ◽

Yanhui Xu ◽

Xiaodan Li ◽

Jiayu Fu ◽

...

Keyword(s):

Confocal Microscopy ◽

Vitamin B12 ◽

Dry Eye ◽

Microscopy Study ◽

Dry Eye Disease ◽

Eye Disease ◽

In Vivo Confocal Microscopy ◽

New Approach

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In Vivo Confocal Microscopy Evaluation of Ocular Surface with Graft-Versus-Host Disease-Related Dry Eye Disease

Scientific Reports ◽

10.1038/s41598-017-10237-w ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 18

Author(s):

Jingliang He ◽

Yoko Ogawa ◽

Shin Mukai ◽

Yumiko Saijo-Ban ◽

Mizuka Kamoi ◽

...

Keyword(s):

Confocal Microscopy ◽

Dry Eye ◽

Graft Versus Host Disease ◽

Ocular Surface ◽

Dry Eye Disease ◽

Eye Disease ◽

In Vivo Confocal Microscopy ◽

Host Disease ◽

Graft Versus Host

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The effect of Polydatin in dry eye disease in vivo and in vitro

The FASEB Journal ◽

10.1096/fasebj.2019.33.1_supplement.lb388 ◽

2019 ◽

Vol 33 (S1) ◽

Author(s):

Bongkyun Park ◽

Tae Gu Lee ◽

Soo‐Wang Hyun ◽

Kyuhyung Jo ◽

Ik Soo Lee ◽

...

Keyword(s):

Dry Eye ◽

Dry Eye Disease ◽

Eye Disease

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Advances in the Noninvasive Diagnosis of Dry Eye Disease

Applied Sciences ◽

10.3390/app112110384 ◽

2021 ◽

Vol 11 (21) ◽

pp. 10384

Author(s):

Luca Di Cello ◽

Marco Pellegrini ◽

Aldo Vagge ◽

Massimiliano Borselli ◽

Lorenzo Ferro Desideri ◽

...

Keyword(s):

Dry Eye ◽

Anterior Segment ◽

Tear Film ◽

Optical Quality ◽

Dry Eye Disease ◽

Diagnostic Tools ◽

Eye Disease ◽

Non Invasive ◽

Integrated Devices

Dry eye disease (DED) is a multifactorial disease that represents one of the most common ophthalmologic conditions encountered in everyday clinical practice. Traditional diagnostic tests for DED, such as subjective questionnaires, tear film break-up time and the Schirmer test, are often associated with poor reproducibility and reliability, which make the diagnosis, follow-up, and management of the disease challenging. New advances in imaging technologies enable objective and reproducible measurements of DED parameters, thus making the diagnosis a multimodal imaging-based process. The aim of this review is to summarize all the current and emerging diagnostic tools available for the diagnosis and monitoring of DED, such as non-invasive tear breakup time, thermography, anterior segment optical coherence tomography, meibography, interferometry, in vivo confocal microscopy, and optical quality assessment. Although there is not a gold standard imaging technique, new multi-imaging-integrated devices are precious instruments to help clinicians to better cope with the diagnostic complexity of DED.

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