scholarly journals Immunological aspects of carbohydrate and lipid metabolism in patients with periprosthetic infection and osteomyelitis

2020 ◽  
pp. 8-15
Author(s):  
P. G. Kravchun ◽  
M. O. Korzh ◽  
I. P. Dunaeva ◽  
F. S. Leontieva ◽  
I. V. Gusakov ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Connective Tissue ◽  
Immune Complexes ◽  
Synovial Membrane ◽  
Periprosthetic Infection ◽  
Circulating Immune Complexes ◽  
Cartilage Tissue ◽  
E Coli ◽  
Carbohydrate And Lipid Metabolism ◽  
Lymphocytotoxic Antibodies

A study of carbohydrate and lipid metabolism and immunological examination of patients with periprosthetic infection after knee and hip joint endoprosthetics (n = 16) and osteomyelitis (n = 20) was conducted. The patients with periprosthetic infection were characterized by anemia, eosinophilia, impaired glucose tolerance, cholestasis, predisposition to elevated atherogenesis, increased circulating immune complexes, autoimmune granulocytotoxic antibodies, immunoglobulins IgM, IgG, IgA and sensitization to synovial membrane and Staphylococcus aureus. The patients with osteomyelitis did not develop increased glucose and cholesterine level, but showed increased aminotransferases, thymol test, chondroitinsulfates, decrease of calcium and production of the leukocyte migration inhibition factor (LIF), both non-specific and to the antigens of cartilage tissue and sensitization to Streptococcus and E. coli. In patients with infections of the musculoskeletal system, glucose metabolism disturbances have been associated with the presence of autoimmune lymphocytotoxic antibodies, reduced production of LiF, both non-specific and to the antigens of synovial membrane. Atherogenesis was associated with increased circulating immune complexes, autoimmune lymphocytotoxic and granulocytotoxic antibodies, decreased LIF and sensitization to connective tissue antigens and pathogenic microorganisms, especially to Streptococcus and Proteus. Anemia was associated with increased autoimmune lymphocytotoxic antibodies, delayed-type sensitization to bone and cartilage tissue, to Staphylococcus and sensitization by accelerated type to E. coli and Proteus. Physicians should analyse immunological data while treating and monitoring the patients with connective tissue infections and disturbances of glucose and cholesterine metabolism.

scholarly journals Immunological Aspects of Carbohydrate and Lipid Metabolism in Patients with Periprosthetic Infection and Osteomyelitis

2020 ◽  
Vol 45 (02) ◽  
pp. 85-92
Author(s):  
Pavlo Kravchun ◽  
Mykola Korzh ◽  
Inna Dunaeva ◽  
Frida Leontieva ◽  
Igor Gusakov ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Lipid Metabolism ◽  
Periprosthetic Infection ◽  
E Coli ◽  
Carbohydrate And Lipid Metabolism ◽  
Störungen Des Glukosestoffwechsels

Zusammenfassung Einleitung Die Behandlung von Patienten mit periprothetischer Infektion und Osteomyelitis ist recht kompliziert. Das Ziel dieser Studie war es, den Kohlenhydrat- und Lipidstoffwechsel sowie das immunologische Profil bei Patienten mit periprothetischer Infektion und Osteomyelitis zu untersuchen. Material und Methoden Eine Studie zum Kohlenhydrat- und Lipidstoffwechsel und zur immunologischen Untersuchung von Patienten mit periprothetischer Infektion nach Knie- und Hüftgelenkendoprothetik (n = 16) und Osteomyelitis (n = 20) wurde durchgeführt. Ergebnisse Patienten mit einer periprothetischen Infektion neigten zu Anämie, gestörter Glukosetoleranz, einer Veranlagung zu verstärkter Atherogenese, Autoimmun-Granulozytotoxizität und einer Sensibilisierung gegen die Synovialmembran und Staphylococcus aureus. Patienten mit Osteomyelitis zeigten eine Abnahme der Produktion des Leukozyten-Migrationshemmungsfaktors (LIF), sowohl unspezifisch als auch spezifisch gegenüber den Antigenen des Knorpelgewebes sowie eine Sensibilisierung gegenüber Streptokokkus und E. coli. Bei den untersuchten Patienten wurden die Störungen des Glukosestoffwechsels mit dem Vorhandensein von autoimmunen lymphozytotoxischen Antikörpern, einer verminderten Produktion von LIF, sowohl unspezifisch als auch spezifisch gegen die Antigene der Synovialmembran in Verbindung gebracht. Die Atherogenese war mit einer erhöhten Anzahl zirkulierender Immunkomplexe, autoimmunen lymphozytotoxischen und granulozytotoxischen Antikörpern, einem verminderten LIF und einer Sensibilisierung gegenüber Antigenen des Bindegewebes, Streptokokkus und Proteus assoziiert. Eine Anämie war mit einem Anstieg der autoimmunen lymphozytotoxischen Antikörpern, einer verzögerten Sensibilisierung gegen Knochen- und Knorpelgewebe, gegen Staphylokokken und einer beschleunigten Sensibilisierung gegen E. coli und Proteus assoziiert. Schlussfolgerungen Ärzte sollten bei der Behandlung und Überwachung von Patienten mit Bindegewebesinfektion und einem gestörten Glukose- und Cholesterinstoffwechsel auch immunologische Aspekte analysieren.

Circulating Immune Complexes in connective tissue diseases

1987 ◽  
Vol 8 (1) ◽  
pp. 1-5
Author(s):  
Antonio Puccetti ◽  
Giorgio Trovatello ◽  
Sebastiana Cantarella ◽  
Franco Celada ◽  
Paola Migliorini ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Immune Complexes ◽  
Connective Tissue Diseases ◽  
Circulating Immune Complexes

An enzymatically active antigen-antibody probe to measure circulating immune complexes. II. E. coli beta-galactosidase in the probe and C1q as the recognition unit

1983 ◽  
Vol 59 (2) ◽  
pp. 245-254 ◽  
Author(s):  
Paola Migliorini ◽  
Giorgio Trovatello ◽  
Sebastiana Cantarella ◽  
Fabrizio Manca ◽  
Stefano Bombardieri ◽  
...  
Keyword(s):  
Immune Complexes ◽  
Circulating Immune Complexes ◽  
E Coli ◽  
Antigen Antibody ◽  
Active Antigen ◽  
Beta Galactosidase

scholarly journals Circulating Immune Complexes in Mixed Connective Tissue Disease

1979 ◽  
Vol 22 (5) ◽  
pp. 484-489 ◽  
Author(s):  
James T. Halla ◽  
John E. Volanakis ◽  
Ralph E. Schrohenloher
Keyword(s):  
Connective Tissue ◽  
Connective Tissue Disease ◽  
Immune Complexes ◽  
Mixed Connective Tissue Disease ◽  
Circulating Immune Complexes

scholarly journals Nonspecific reactivity of the rabbits organism when exposed to cysticercosis

2019 ◽  
Vol 21 (94) ◽  
pp. 132-135
Author(s):  
Y. V. Duda
Keyword(s):  
Blood Serum ◽  
Bactericidal Activity ◽  
Immune Complexes ◽  
Research Group ◽  
Phagocytic Activity ◽  
Lysozyme Activity ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
E Coli ◽  
Test Culture

One of the actual rabbits breeding problem is the reduction of their resistance, which is caused by the spread of individual invasive diseases, especially cysticercosis, which is caused by the larval Cysticercus pisiformis stage of the mature cestode Taenia pisiformis. The study was conducted on 38 male rabbits age 3–4 months, weighing 3.5–4.0 kg of the Californian breed, selected by analogy. Animal were separated into two groups: healthy animals (control group) and sick animals (research group). After blood collection, slaughter was conducted and the presence of cysticercus cysts was visually determined in the organisms of the rabbits. Control animals received a balanced standard granular feed and water without limit; research group in addition to the standard granulated feed with water received flaccid hay. The animals were kept in mesh single-tier cages in the room, according to the current veterinary and sanitary standards. The definition of phagocytic activity of neutrophils was carried out with the addition of standardized to 2500000000/ml suspension of daily culture of E. coli 055K59№3912/41. The bactericidal activity of blood serum was determined by the method Smirnova A.V. and Kuzmina T.A. in relation to the E. coli microbial test-culture 055K59№3912/41. The serum lysozyme activity was determined by the Nephelometric method using the Dorofachuk V.G. method to the microbial test culture Micrococcus luteus ATSS9341. Circulating immune complexes were determined using polyethylene glycol in borate buffer (pH 8.4). It was found that in blood of sick animals phagocytic activity is lower than in blood of healthy ones (respectively, 41.82 ± 1.51% versus 47.38 ± 1.10%, P < 0.01). The main cells involved in phagocytosis are leukocytes, in particular neutrophils and eosinophils. A low indicator of phagocytic activity shows depressed phagocytosis in the organism of animals suffering from cysticercosis of rabbits. The phagocytic number in the blood of rabbits of the experimental group was significantly lower by 0.51 units (P < 0.05) as compared to the control group and correlated with the index of phagocytic activity. An important element of immunity are indicators of bactericidal and lysozyme activity of blood serum. Low bactericidal activity of serum by 12.88% (P < 0.01) and lysozyme activity in rabbits with Cysticercus pisiformis also indicates a weakening of the factors of nonspecific natural resistance of the organism. Analyzing the level of circulating immune complexes, we found a high level of medium (9.14 ± 0.40 vs 5.58 ± 0.50) and small (9.21 ± 1.35 vs 4.64 ± 0.68) CIC for cysticercosis, respectively, 1.64 (P < 0.01) and 1.98 times (P < 0.001) against the control. This indicates the inhibition of the immunobiological activity in the organism of rabbits as a result of the combination of specific antibodies with the products of the exchange of helminths.

scholarly journals Studies on circulating immune complexes and their composition in patients with mixed connective tissue disease(MCTD).

1988 ◽  
Vol 11 (2) ◽  
pp. 128-136
Author(s):  
Takashi Abe ◽  
Yoshikazu Akahonai ◽  
Toshikazu Hosokawa ◽  
Takayuki Nomura ◽  
Masamitsu Mori ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Connective Tissue Disease ◽  
Immune Complexes ◽  
Mixed Connective Tissue Disease ◽  
Circulating Immune Complexes
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