scholarly journals The inhibitory effect of trans-10,cis-12 conjugated linoleic acid on sterol regulatory element binding protein-1 activation in bovine mammary epithelial cells involved reduced proteasomal degradation of insulin-induced gene-1

2021 ◽  
Author(s):  
Liang Chen ◽  
Andrea J. Lengi ◽  
Benjamin A. Corl
Keyword(s):  
Linoleic Acid ◽  
Epithelial Cells ◽  
Conjugated Linoleic Acid ◽  
Mammary Epithelial Cells ◽  
Regulatory Element ◽  
Inhibitory Effect ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells ◽  
Element Binding Protein ◽  
Sterol Regulatory Element

scholarly journals β-Hydroxybutyrate Facilitates Fatty Acids Synthesis Mediated by Sterol Regulatory Element-Binding Protein1 in Bovine Mammary Epithelial Cells

2015 ◽  
Vol 37 (6) ◽  
pp. 2115-2124 ◽  
Author(s):  
Min Zhang ◽  
Shiqi Zhang ◽  
Qi Hui ◽  
Lin Lei ◽  
Xiliang Du ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Epithelial Cells ◽  
Fatty Acid Synthase ◽  
Mammary Epithelial Cells ◽  
Regulatory Element ◽  
Lipid Synthesis ◽  
Mammary Epithelial ◽  
Control Group ◽  
Bovine Mammary Epithelial Cells ◽  
Sterol Regulatory Element

Background/Aims: In dairy cows, β-hydroxybutyrate (BHBA) is utilized as precursors of de novo synthesized fatty acids in mammary gland. Ketotic cows are characterized by excessive negative energy balance (NEB), which can further increase the blood BHBA concentration. Sterol regulatory element-binding protein1 (SREBP1) and cell death-inducing DNA fragmentation factor-alpha-like effector α (Cidea) play crucial roles in lipid synthesis. Therefore, we hypothesized that BHBA could stimulate SREBP1/Cidea pathway to increase milk fat synthesis in bovine mammary epithelial cells. Methods: Bovine mammary epithelial cells were treated with different concentrations of BHBA and transfected with adenovirus to silence SREBP1 expression. The effects of BHBA on the lipid synthesis in bovine mammary epithelial cells were investigated. Results: The results showed that BHBA could significantly increase the expression of SREBP1, fatty acid synthase (FAS), acetyl-CoA carboxylase α (ACC-α), Cidea and diacylglycerol transferase-1 (DGAT-1), as well as the triglycerides (TG) content in bovine mammary epithelial cells. BHBA treatment also increased the transfer of mature SREBP1 to nucleus compared with control group. However, SREBP1 silencing could significantly down-regulate the overexpression of FAS, ACC-α, Cidea and DGAT-1, as well as TG content induced by BHBA. Conclusion: The present data indicate that BHBA can significantly increase TG secretion mediated by SREBP1 in bovine mammary epithelial cells.

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